EMAGE mouse gene expression spatial database

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EMAGE:19065

Jag1 jagged 1 ( MGI:1095416)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_015945. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_015945_01	euxassay_015945_02	euxassay_015945_03	euxassay_015945_04

euxassay_015945_05	euxassay_015945_06	euxassay_015945_07	euxassay_015945_08	euxassay_015945_09

euxassay_015945_10	euxassay_015945_11	euxassay_015945_12	euxassay_015945_13	euxassay_015945_14

euxassay_015945_15	euxassay_015945_16	euxassay_015945_17	euxassay_015945_18	euxassay_015945_19

euxassay_015945_20	euxassay_015945_21	euxassay_015945_22	euxassay_015945_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19065_wholemount_strong.wlz

19065_wholemount_moderate.wlz

19065_wholemount_weak.wlz

19065_wholemount_possible.wlz

19065_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19065_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

epidermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

pineal primordium

strong

single cell

strong expression: see section 11

diencephalon lateral wall ventricular layer

strong

single cell

strong expression: see section 10

olfactory cortex mantle layer

weak

regional

weak expression: see section 07 08 09 12 13 14 15

telencephalon ventricular layer

moderate

regional

moderate expression: see section 01 weak expression: see section 02 06 15 16 21

medulla oblongata basal plate ventricular layer

moderate

single cell

moderate expression: see section 13 weak expression: see section 10

pons ventricular layer

strong

single cell

strong expression: see section 08 moderate expression: see section 13 14 weak expression: see section 10 15

midbrain ventricular layer

strong

single cell

strong expression: see section 07 08 09 10 11 12 13 14 15 16

intermediate grey horn

strong

single cell

strong expression: see section 15 moderate expression: see section 13 14

inner ear vestibular component

moderate

regional

moderate expression: see section 01 02 05 06 07 17 19

pharyngo-tympanic tube

weak

regional

weak expression: see section 01 21 22 23

extrinsic ocular muscle

weak

regional

weak expression: see section 02 03 04 18

lens

moderate

regional

moderate expression: see section 23

aorta

weak

regional

weak expression: see section 10 11 12 13 14 15 16

metanephros

strong

regional

strong expression: see section 08 09 10 11 17 moderate expression: see section 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T63337
Entity Detected:	Jag1, jagged 1 ( MGI:1095416)
Sequence:	sense strand is shown >T63337 GACATGGATAAACACCAGCAGAAAGTCCGCTTTGCCAAACAGCCAGTGTATACGCTGGTAGACAGAGAGG AGAAGGCCCCCAGCGGCACGCCGACAAAACACCCGAACTGGACAAATAAACAGGACAACAGAGACTTGGA AAGTGCCCAGAGCTTGAACCGGATGGAATACATCGTATAGCAGACAGTGGGCTGCCGCCATAGGTAGAGT TTGAGGGCACCGCGGGGCTTGTAGTTTCTTTAAACTGTTGTCCTATTCCAGTCTGAGGCTGTTGTTGACT TAGAATCCTGTGTTAATTTAAGTTTCGACAAGCTGGCTTCCACTGGCACTGGTAGTTTCTGTGGTTGGCT GGGAAATTGAGTGCAGCGCTCTCACAGCTATGCAAAAACTAGTGAATGTGCCCTGGTGTCCATTCCCCTG CCGCAGACACATCGGGCTCCCAGGAGCTGCCCAGCCCTAGGCCTGGAGCTTCCACATCTGCCAGCGGTCC TAATGGTGATGGCAGCCTTAGGATCATAGTTTTATTTATATTTATTGACTCTTGAGTTGTTTTTGTATAT TGGTTTTATGATGACGTTCGAGTAGTTCTGTATTTGAAAGTGCCTTTGCAGCTCAGAACCACAGCAACTA TCACAAATGACTTTATTATTTATTTTTTTTTATTGTATTTTTGTTGTTGGGGGAGGGGGGATTTGATGTC AGCAGTTGCTGGTAAAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 96284. Forward Primer - name:096284_F_cDNA_Jag1, sequence:GACATGGATAAACACCAGCAGA; Reverse Primer - name:096284_N_SP6_cDNA_Jag1, sequence:TTTTACCAGCAACTGCTGACATC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19063 same embryo
	EMAGE:19064 same embryo
	EMAGE:19062 same embryo
	EMAGE:19066 same embryo
	EMAGE:19067 same embryo
	EurExpress:euxassay_015945 same experiment
	MGI:4825671 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source