EMAGE mouse gene expression spatial database

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EMAGE:19184

Fbxo32 F-box protein 32 ( MGI:1914981)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009279. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009279_01	euxassay_009279_02	euxassay_009279_03	euxassay_009279_04

euxassay_009279_05	euxassay_009279_06	euxassay_009279_07	euxassay_009279_08	euxassay_009279_09

euxassay_009279_10	euxassay_009279_11	euxassay_009279_12	euxassay_009279_13	euxassay_009279_14

euxassay_009279_15	euxassay_009279_16	euxassay_009279_17	euxassay_009279_18	euxassay_009279_19

euxassay_009279_20	euxassay_009279_21	euxassay_009279_22	euxassay_009279_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19184_wholemount_strong.wlz

19184_wholemount_moderate.wlz

19184_wholemount_weak.wlz

19184_wholemount_possible.wlz

19184_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19184_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

sublingual gland primordium

strong

regional

strong expression: see section 11 12 13

telencephalon ventricular layer

weak

regional

weak expression: see section 03 04 05 06

midbrain ventricular layer

weak

regional

weak expression: see section 08 09 10 11 12 13 14

intermediate grey horn

moderate

single cell

moderate expression: see section 05 06 07 08 09

cochlea

strong

regional

strong expression: see section 03 04 05 15 16 17 moderate expression: see section 01 02 14

cochlear duct

strong

regional

strong expression: see section 13

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 10 12 13 14 15 16

vomeronasal organ

strong

regional

strong expression: see section 09 12

heart atrium

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12

esophagus

weak

regional

weak expression: see section 07 08

lower lip

moderate

regional

moderate expression: see section 06 07 13 14 15

tail mesenchyme

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3490
Entity Detected:	Fbxo32, F-box protein 32 ( MGI:1914981)
Sequence:	sense strand is shown >T3490 TGGCCTCGAGCCAGATTCGGCACGAGGGCAANCGTTTGATCTTGTCTGACAAAGGGCAGCTGGATTGGAA GAAGATGTATTTTAAGCTTGTACGATGTTACCCAAGAAGAGAGCAGTATGGGGTCACCCTGCAGCTTTGC AAACACTGCCACATTCTCTCCTGGAAGGGCACTGACCATCCGTGCACGGCCAACAACCCAGAGAGCTGCT CCGTCTCACTTTCCCCTCAAGACTTTATCAATTTGTTCAAGTTCTGAATAATCCCAGCACACGACAACAC TTCAGAAGGCTTCTAATTGGATGGCTGGGAGTCGGGACACTTCATTTGTAAATAGTGTACATTTTAAGCA TTGGCTTGAAACTGCGGGGGATACGTCATTGAGGAGACGTTGGCGGGGAAGAGATGCAGTTGCCGATGGA AATTTACAAATGTGAATTCCACATGAGAACTGGTACAGAAAAGCAGAAATACTGTAAATAGACTTTTTAT TTTCCCTAACGATTTGCAAGCAAGACTATAAAGGCAAGAACTCTATGTCAGCCATGGAAACGG
Notes:	The probe template was PCR amplified from IMAGE:333866 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:333866 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19186 same embryo
	EMAGE:19185 same embryo
	EurExpress:euxassay_009279 same experiment
	MGI:4824805 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source