EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19249

Aox4 aldehyde oxidase 4 ( MGI:1919122)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009194. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009194_01	euxassay_009194_02	euxassay_009194_03	euxassay_009194_04

euxassay_009194_05	euxassay_009194_06	euxassay_009194_07	euxassay_009194_08	euxassay_009194_09

euxassay_009194_10	euxassay_009194_11	euxassay_009194_12	euxassay_009194_13	euxassay_009194_14

euxassay_009194_15	euxassay_009194_16	euxassay_009194_17	euxassay_009194_18	euxassay_009194_19

euxassay_009194_20	euxassay_009194_21	euxassay_009194_22	euxassay_009194_23	euxassay_009194_24

euxassay_009194_25	euxassay_009194_26

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19249_wholemount_strong.wlz

19249_wholemount_moderate.wlz

19249_wholemount_weak.wlz

19249_wholemount_possible.wlz

19249_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19249_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

anterior naris

strong

regional

strong expression: see section 13 14 16 17

external naris

strong

regional

strong expression: see section 12 13 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3496
Entity Detected:	Aox4, aldehyde oxidase 4 ( MGI:1919122)
Sequence:	sense strand is shown >T3496 TGGCCTCGAGCAGATTCGGCACGAGGGTTTTCATTGTCCTGAAAGTATGCATCATGGTGAGTTTTTAAAC CTAGCTAACTAACCTGGGGTAGCTCAGGAGGGCCTCCTTGTGTTTTGTTTCAAGCAGCTATGTTTGAAAC CCAACAGAGCTGTCTGTTCCAAAACTCTCTCTAGACTGGAGTCTCCCAAAACCAGCACACCTGAAAAACC CACTTTCCAGTGCTTGCCACTTCCTGTGGTACAGACGCTATCATAAGAGCAAGCAGTAAGTGCTCAGGGA ACCTGGACGTAGAGCTGGAGACAGGTTTGAATACAGCTTGTCTCCAAAGGTGTGTGCTGGAAGCCTGGCT CCCAGGGTGGTACTGTTGAAACTGTAAGAGGTGGAGCCTATTGAGAGGTCATTGGTAAGGGATCAATGGT GACAGGAAGGAATGGACTAGTTCCCCAAAACCTGGCCCCTCCTCCTCTCTGCCAGTGTGAGCCATTGCAT TTCTAGTGTGTGATGCCATCTACTGGGACAGAAAGGAGGTCCTCACCAGATGGGCAGTCTTGTGAACTTG CCTCTAAACTA
Notes:	The probe template was PCR amplified from IMAGE:334519 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:334519 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19251 same embryo
	EMAGE:19248 same embryo
	EMAGE:19250 same embryo
	EMAGE:19252 same embryo
	EurExpress:euxassay_009194 same experiment
	MGI:4823158 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source