EMAGE mouse gene expression spatial database

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EMAGE:19323

Tnk2 tyrosine kinase, non-receptor, 2 ( MGI:1858308)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002030. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002030_01	euxassay_002030_02	euxassay_002030_03	euxassay_002030_04

euxassay_002030_05	euxassay_002030_06	euxassay_002030_07	euxassay_002030_08	euxassay_002030_09

euxassay_002030_10	euxassay_002030_11	euxassay_002030_12	euxassay_002030_13	euxassay_002030_14

euxassay_002030_15	euxassay_002030_16	euxassay_002030_17	euxassay_002030_18

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19323_wholemount_strong.wlz

19323_wholemount_moderate.wlz

19323_wholemount_weak.wlz

19323_wholemount_possible.wlz

19323_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19323_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 14 15 16

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

vibrissa

moderate

regional

moderate expression: see section 06 07 18

spinal cord

moderate

homogeneous

moderate expression: see section 09 10 11 12 13

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4571
Entity Detected:	Tnk2, tyrosine kinase, non-receptor, 2 ( MGI:1858308)
Sequence:	sense strand is shown >T4571 CCCACGCGTCCGGCCCCAGGCTCCTGCTGGCTTCAGCTGCTAATACTGCCACAGCGGATGGGTCAACTTC TAGACTGCCTTCTTCTGAGGTGCCAGCTTTTACTCGGTCCCTAGAACTGCTCAGGAGGTGGGAGGGCTGA GAACTACTGGTGGCGTGGGCAGAATACGCGGACCCTGTGTGTAGGACCCTTCCCTCGAAATGTCGTGACC TCCGTGGCTGGCCTGTCAGCCCAGGACATCAGCCAGCCTCTACAGAATAGCTTCATTCACACAGGACATG GTGACAGTGACCCCCGCCACTGCTGGGGGTTCCCTGACAGGATCGATGAACTGTACCTGGGAAACCCCAT GGACCCTCCTGACCTGCTGAGTGTGGAACTGAGCACCTCCCGACCCACCCAGCACCTAGGACGGGTGAAA AAGCCAACATACGACCCTGTGAGTGAGGACCCAGACCCCCTGTCCAGCGACTTCAAGAGGCTTGGCCTGA GGAAGCCAGCCCTGCCTCGAGGGCTGTGGCTGGCAAAGCCCTCAGCCCGAGTGCCAGGCACCAAGGCAGA CCGCAGCAGTGGGGGTGAGGTCACACTCATCGACTTCGGTGAGGAGCCTGTGGTCCCAACCCCTCGGCCC TGTGCACCCTCCTTGGCACAGTTGGCCATGGATGCCTGCTCCTTGCTGGACAAGACACCACCACAGAGCC CCACACGGGCACTGCCACGACCTTTACACCCCACCCCTGTGGTAGACTGGGACGCGCGTCCGCTGCCCCC GCCCCCTGCCTATGACGACGTGGCCCAGGATGAGGATGACTTTGAGGTCTGCTCCATCAACAGCACGCTA GTAGGTGCAGGCCTCCCTGCTGGGCCTAGCCAAGGCGAGACCAATTACGCCTTTGTACCTGAGCAGGCGC AGATGCCCCCTGCCCTGGAGGATAACCTGTTCCTTCCACCCCAGGGCGGAGGCAAGCCGCCCAGCTCAGT GCAGACTGCAGAGATTTTCCAGGCACTGCAGCAGGAGTGTATGCGGCAGCTACAGGTCCCCACTGGCCAG CTGACCCCCTCCCCGACCCCAGGAGGTGATGACAAGCCCCAGGTGCCACCCCGGGTACCTATTCCCCCTC GGCCCACGCGTCCACGTGTGGAGCTATCTCCAGCTCCCTCGGGTGAGGAAGAGACAAGCCGGTGGCCTGG ACCTGCCTCGCCCCCCCGAGTGCCTCCCCGGGAACCTCTGTCTCCTCAAGGTTCAAGGACCCCAAGCCCC CTAGTGCCACCTGGCAGCTCTCCACTACCGCATCGGCTCTCTAGCTCACCTGGAAAGACCATGCCCACCA CTCAAAGCTTTGCCTCAGACCCTAAGTATGCCACTCCACAAGTGATCCAGGCTCCTGGCCCACGGGCAGG CCCCTGCATCCTGCCCATTGTCCGCGATGGCAGGAAGGTCAGCAGCACTCATTACTACCTGCTGCCTGAG CGCCCTCCTTACCTGGAACGCTATCAGCGCTTCCTGCGGGAGGCCCAGAGCCCGGAAGAGCCGGCCGCCC TGCCCGTGCCCCCGCTGTTGCCCCCGCCCAGTACTCCAGCCCCTGCTGCCCCCACTGCCACCGTCAGACC TATGCCTCAGGCCGCCCCAGACCCAAAGGCCAACTTCTCCACCAATAACAGCAACCCAGGGGCACGGCCA CCATCCCTGAGGGCCGCGGCTCGGCTGCCACAGAGGGGCTGCCCAGGGGACGGGCAAGAGGCTGCTCGGC CAGCAGACAAGGTCCAGATGCTGCAGGCCATGGTGCATGGGGTGACCACAGAGGAGTGCCAGGCGGCCCT GCAGAGCCACAGCTGGAGTGTTCAGAGGGCTGCCCAGTATCTGAAGGTGGAGCAGCTCTTTGGGCTGGGT CTTCGGCCACGGGTGGAGTGCCACAAGGTCCTAGAGATGTTCGACTGGAACCTAGAGCAAGCCGGCTGTC ACCTTCTGGGCTCCTGTGGCCCTGCTCATCACAAGCGCTGATGGTTGACTGGAGAGCAGTGGTCTGCCCT AAAAGAACCTCTTGAATCTGTCTGTGGGACAAGGGTGGGGGAGCTGCCCTGCCTCAGGCCCGAAGGACCC ACAACTGCCTGCGGCTCCCAGGGGACAGTGAGACCCAGGCAGCAGGAGGCTGGGAGCCTCACCTTGCCCA CCTCGCTCACCCACCCAGCGCTGTTCTGCACACCTTGGTTCAGCCCTCAGTGCCCCAAGAGGTGGGACGT TCCCAGCCTTGTGAAGGCAAGACAGGAAGCTGCCTGGCAGGCTCTGTGTGGCCTGCCTCTGGCTCCAGAT CAAGGCTGGGCTGGTGGCTAGAGTGTGCCCCAGGCTTTCCTCTGGGGGATTAGACTGACAGGAAGCCAGG CTTGACATGGACAAGAAGGATGTGTTGGCCACACAGCAGGGCGAGCCAGCAACCAACAGGCCCTCCTCAG GGCTAGGCCCCTTCCCAGGGAACCCCTGTAGGAGTGTGGAGTGTTGGACAGAATGTGACCTGTAGCTTCA CTGTGAACTTGTGGGCTTGGCTGGTCTTAGGAACTTGTACCTATAAACAGCCTGAAGTGGCTCAGAAACA GAAAAGGTGCCAGGATCTTCTCTGGCAGGGACCAGGGCCCAAGGCCCCGGATGTGGAAGGAGACCCCAGT GGCACCAGTACTTTCCTGTTTGACCTGTTCCTCCCCTGTTGCTATCTGATGTGTTCCCATCAGCCTCTGT TGCCAAAGTCGTGGCCCCAGCTATGGATATCTGCTTCTTCCAGAGAAATAAAGCTTGTTTCTATTTTATG TTAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4984168 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4984168 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19324 same embryo
	EMAGE:19321 same embryo
	EMAGE:19322 same embryo
	EurExpress:euxassay_002030 same experiment
	MGI:4828851 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source