EMAGE mouse gene expression spatial database

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EMAGE:19324

Xkr6 X Kell blood group precursor related family member 6 homolog ( MGI:2447765)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001951_01	euxassay_001951_02	euxassay_001951_03	euxassay_001951_04	euxassay_001951_05

euxassay_001951_06	euxassay_001951_07	euxassay_001951_08	euxassay_001951_09	euxassay_001951_10

euxassay_001951_11	euxassay_001951_12	euxassay_001951_13	euxassay_001951_14	euxassay_001951_15

euxassay_001951_16	euxassay_001951_17	euxassay_001951_18	euxassay_001951_19

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17

telencephalon mantle layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

hindbrain

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

midbrain

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

facial vii ganglion

strong

regional

strong expression: see section 05 18

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 16 moderate expression: see section 07

trigeminal v ganglion

strong

regional

strong expression: see section 05 06 07 08 09 15 16 17 18 19 moderate expression: see section 04

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 08 15 16 17 moderate expression: see section 07

spinal cord

moderate

homogeneous

moderate expression: see section 09 10 11 12 13

cervico-thoracic ganglion

weak

regional

weak expression: see section 09 13 14

cervical ganglion

weak

regional

weak expression: see section 09 14 15

thoracic ganglion

weak

regional

weak expression: see section 10 11 12

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4569
Entity Detected:	Xkr6, X Kell blood group precursor related family member 6 homolog ( MGI:2447765)
Sequence:	sense strand is shown >T4569 CGCAGCCCTTCCGTGTTCAGGCAACTGCAATCTCCTGGCTCTTACACTGATTGTTGTGAGATCTGGTACT CCTAGAGGAGACACAGGGTGACAGGAGGTGACATAAGAAGAGGAGATAGTAAATCCAGGAACAGCCCGGT GGGCGTCTTCTCTCCTGGGCTGCCTTCCTGGTCCAGCTTTCTCAGTAGGGGAGAACTCGGCACTCACCAT GCCTTCAGTTGGAACCCCGGTACAAATTGACCAACATGCACTGCAGCTAGCAGGTGGCCCCTGCATCCAG CACTGCCGCATAGGAGGGAGCAGGCCCTAAGGTATATCCGCACCATGTACCTGGGGATTCAGAGCCAGCG GCAGAAGGAACACCAGCGGCGCTTCTACTGGGCTATGATGTACGAATATGCAGATGTCAACATGCTGCGC CTCCTGGAGACCTTTCTGGAGAGCGCCCCCCAACTGGTGCTACAGCTCTGCATAATGATCCAGAAGAACA GTGCAGAGACGCTGCCCTGTGTCTCCTCTGTGACTTCCCTGATGTCCCTGGCTTGGGTGCTAGCTTCCTA TCACAAGCTGCTGCGGGACTCCAGGGACGACAAGAAGAGCATGAGTTACAGAGGGGCCCTCATCCACCTC TTCTGGCGCCTCTTCACCATCTCATCCCGAGTTATCTCTTTCGCCCTCTTTGCTTCCATCTTCCAGCTGT ATTTCGGGATCTTCGTGGTGGTGCACTGGTGCGCCATGGCCTTCTGGATCATCCATGGTGGGACAGACTT CTGCATGTCCAAGTGGGAGGAGATCCTCTTCAACATGGTGGTAGGGATCGTGTACATTTTCTGCTGGTTT AACGTCAAGGAAGGGCGGACTCGATACCGAATGTTCGCATACTATACGATAGTCTTGACCGAGAATGCTG CCTTGACTTTCCTTTGGTATTTTTACAGAAATCCGGAGAGCACTGACTCCTATGCCGTGCCAGCACTGTG TTGTGTCTTCGTTAGCTTTGTGGCTGGGATTACGCTCATGCTCTTATACTATGGCGTGTTGCATCCCATG GGGCCGCGGGCTAAGGTCTTTGCCAGCTCCTGTTGTGCCGAGCTGCTCTGGGGCATCCCTTTGCCCCCTG ATGTTGAGCCTATGGCACCTCAGACCCCTGGGTACCGGGGGACCCAGGTCACGCCCACCAGAGCCGTGAC GGAACAGCAGGAGGACCTCACGGCTGATACTTGCCTGCCGGTATTCCAAGTGAGACCCATGGGGCCCTCT ACCCCGTCAGGGCGTCCTTACCACCCAGAAGGGCCCCTGATTAAGATTGACATGCCAAGAAAGCGATACC CGGCCTGGGATGCGCACTTTGTAGACAGGAGGCTGCGGAGGACTATTAACATCCTGCAGTACGTCACCCC CACCGCAGTGGGCATTCGCTACCGAGATGGGCCTCTGCTCTATGAGCTGCTGCAGTATGAGTCTTCACTC TAGAACACCTTGACCCAGATTGAGAAGGGGACCTTAAGTTTGGTTGCGGTCAACGCCGCCTGCAAGAAAT ATAACCCTCCCTTCCCCCAATACACAGAACCACCACCACCACCGCCACACCAACAACACCAACACTACAA AAAAGATCAATAAGTCACATCCCCTTCAGATAAGCTTTCTTTCCAGTCACTATATGTATACAAAGATATT CTCTATGTTTTGTAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4983341 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4983341 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19321 same embryo
	EMAGE:19323 same embryo
	EMAGE:19322 same embryo
	EurExpress:euxassay_001951 same experiment
	MGI:4829240 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source