EMAGE mouse gene expression spatial database

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EMAGE:19364

Acbd4 acyl-Coenzyme A binding domain containing 4 ( MGI:1914381)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002157. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002157_01	euxassay_002157_02	euxassay_002157_03	euxassay_002157_04

euxassay_002157_05	euxassay_002157_06	euxassay_002157_07	euxassay_002157_08	euxassay_002157_09

euxassay_002157_10	euxassay_002157_11	euxassay_002157_12	euxassay_002157_13	euxassay_002157_14

euxassay_002157_15	euxassay_002157_16	euxassay_002157_17	euxassay_002157_18	euxassay_002157_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19364_wholemount_strong.wlz

19364_wholemount_moderate.wlz

19364_wholemount_weak.wlz

19364_wholemount_possible.wlz

19364_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19364_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5508
Entity Detected:	Acbd4, acyl-Coenzyme A binding domain containing 4 ( MGI:1914381)
Sequence:	sense strand is shown >T5508 CGTTCGCCCCTACTACTCCAGTGTCCTTTCTGCGGAACGCCGGGCTCCAGGACTCTCCTCTATTGCCAGC GTAGACGTGTTCCAAGAGCTGAGTCGAGGTGCCTCGGACAGGCGGAGCCCGCATCAATCAACCCACTCCC GTGGGTTTCCACCAGGGGGCGCGAGGACTCTGCGACCATCAGTGTGTCCACCGGGAGGCGCTGCAGCCCG CCTCGGGCTGTCGCGCGGTGCGGAGTGCCACCCATAAATTAGCCCCGCCTGGCTGGGCGAGGCAGGATAG CATTATTTGCATAATCTTGAAAGAGCCTTCCTTACTGGTTAGGGTATAAAAGACCAATACGAAACGGTGC CTCTGGTTGGCTTTTCTCCTAGCTCTGTATCCCGCCCTATCCCTGCCCCTCTGGAACAAGAGGTCAATCG GAAGCCTCTGAGTCGCTTCAAAGTAGGGCTCCAGAGCTCGAAGCAGCATGGGTACCGAGAAGGAAGAGCC CGACTGTCAAAAACAGTTCCAGGCAGCAGTCAGCGTCATTCAGAACCTGCCTAAGAATGGCTCTTACCGC CCCTCCTATGAAGAGATGCTGCGATTCTACAGCTACTACAAGCAGGCTACCATGGGGCCCTGCCTGGTCC CCCGGCCTGGGTTCTGGGACCCCATTGGACGTTACAAGTGGGATGCCTGGAACAGACTGGGCAAGATGAG CAGGGAGGAAGCCATGTCTGCCTACATCTCTGAGATGAAGCTGGTGGCACAGAAGGTGATCGACACAGTG CCCCTGGGCGAGGTGGCAGAGGATATGTTTGGTTATTTCGAGCCCCTGTACCAGGTGATCCCTGACATGC CGAGGCCCCCAGAAACCTTCTTGAGAAGGGCCACAGGTTGGAAGGAGCCGTTGGTGCAGAGAGAGGATCA GGCGGCTCCAGAGCCTTCTTGTGTCCCCAAGGAGCCAGTGCCTCCAAGTCCAGAGTCCCGGCCACCCAGA GACCTGGACCTGGAGGTTTTCTGTGACTCCGTGGAACAGCTAGAGCCAGAGTTGGTGAGACTGCCTGTCT TGTCCCCCGTTGCTGCTGAGCCCGAGTTGTGCCACCTCCCCCCTGGGATCTGGGACTCCTCAGCTCAGCA GGTTTGGGCAGAGCAGAAGGAAGCAGCTGGCAGAGAGCTGACCACCAGGAGCAGCCCCGAGTCCACTGGG GAGAAGGAGGGGTTGGGAGGCGGCCTGATAGGCCCACAGGAGTTGGACACATGGCTGGTGGGGACAGTCC GGGCCATGCAGGAAAGCATGAAGGACGTTCACAGGAGGCTACAGAGCCTGGAGAGCAAGCCCCAGCCACT CAAGCAGAGGTCGCCCAGGACTCGGCCTTGGCCCCTAGGGCTCTCCGTTCCCACGCTGCTCTTCTTTATC CTGTGGCCCTTCGTTGTCCAGTGGCTCTTCCGACAGTTTCGGACCCAGAAGAGGTGACTCAAGGACGGGG TCTCTCTAGCAACTGAGAAGACTATGATGAGTCCCCGAAAGGTCCTGCTGTGCCCTGAGCCTCCCTAGGT TTAGAAAAACAACTTTAGAAATTCCTCCTCCCACCCTCGTAAACCCTGGCACTCCCTCCTTTAATGGGGG CTCCCATAAGACCCATCTTTCCCTGCAGCTCACACCTACGCCCCAAAAGCAGTTCCCAAAGCAGTCATGG TGCACATCCTAACTTTGGGGTTGGCGGTGAGCCGGCCCCACTCTCTGGGTCACCTGACTTTGCTCCCGAC GACTCGGCTCCCTGCCGAGGCTCAGCTCTTGGGCAAGTTGGGACTTGGACCTGGCCTGAAGACTGATTGG CTGGAAGGTTGGTTGCAGGAAAGACCGGAGCCCCGCAGGAGTGGGGAGGAATGGGAGGAGTGTGTACGCT TGGGGGCTGGGATGCCGTGTACACACGCACAAGTACACATCGCGCTCAGGACTTCACTGAAGATTCACGT GCAATTGAACGCTTCATTAAACAAAAGAAAACCTCAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6396076 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6396076 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19359 same embryo
	EMAGE:19360 same embryo
	EMAGE:19361 same embryo
	EMAGE:19362 same embryo
	EMAGE:19363 same embryo
	EurExpress:euxassay_002157 same experiment
	MGI:4822909 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source