EMAGE mouse gene expression spatial database

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EMAGE:19479

Nop2 NOP2 nucleolar protein homolog (yeast) ( MGI:107891)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004863. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004863_01	euxassay_004863_02	euxassay_004863_03	euxassay_004863_04

euxassay_004863_05	euxassay_004863_06	euxassay_004863_07	euxassay_004863_08	euxassay_004863_09

euxassay_004863_10	euxassay_004863_11	euxassay_004863_12	euxassay_004863_13	euxassay_004863_14

euxassay_004863_15	euxassay_004863_16	euxassay_004863_17	euxassay_004863_18	euxassay_004863_19

euxassay_004863_20	euxassay_004863_21	euxassay_004863_22	euxassay_004863_23	euxassay_004863_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19479_wholemount_strong.wlz

19479_wholemount_moderate.wlz

19479_wholemount_weak.wlz

19479_wholemount_possible.wlz

19479_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19479_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

thymus primordium

strong

homogeneous

strong expression: see section 13 14 15 16 17 18

submandibular gland primordium

strong

regional

strong expression: see section 09 10 11 17 18 19 20

vibrissa

moderate

regional

moderate expression: see section 05 06 07 08 19 20 21 22

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 09 10 14

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20 21 22 23

midbrain ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 14 16 17 18

lower jaw incisor

strong

regional

strong expression: see section 12 13 16 17

lower jaw molar

strong

regional

strong expression: see section 09 20

upper jaw incisor

strong

regional

strong expression: see section 11 12 15 16

upper jaw molar

strong

regional

strong expression: see section 09 18 20 moderate expression: see section 19

liver lobe

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

renal cortex

moderate

regional

moderate expression: see section 10 11 12 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6545
Entity Detected:	Nop2, NOP2 nucleolar protein homolog (yeast) ( MGI:107891)
Sequence:	sense strand is shown >T6545 GGAGCGGGCCTTGACTGGAGGTGCCGGGTCAGCCGGAACCAGGTCTAGGCGAGCCGAGCCTTCAGTTATC ATGGGGCGCAAGTTGGATCCTACGAAGAAGGAGAAGCGTGGACCCGGCCGAAAGGCCCGAAAACAGAAGG GTGCGGAGACCGAATTGGTCAGATTTTTGCCTGCAGCTGGCGATGAGAATTCCAAGAGGCTGTCGAGTCG TGCCCGAAAGCGGGCAGCCAAGAGGAGGGCAGGGTCTGTTGACGTCCCTAAGCCAAATAAGTCTCCTGGG ATCAAAACATTGCCTGGAGAGTTATCCAAAGGAGCAGTCCAGGCTCGAGGTAAGAAGCGTCCAGCACCAA TTCAAAACAGTGATGGGGACGAGGAGGAAGACTCGGGGGAAGACGATGTGGTGACCCAGGGGGACCTTTG GGGCTCTGAGGACAGTGATGAAGATATGGTGGATGACTATGGAGCTGCCTCTAACTCGGAGGATGAGGAG GAAAAGCTGTTGCCTATTGAAAGAGCTGCCCTGAAGCAGAAAGCCCAGGATGCCACAGCTGGGGTCCTGT GGAATGAAGAGGACACTGATGAAGATGAAGATGATGATGGTGTGTCCCCTGAGTCCCACCCCAGAAAGGA TGACAAAGCAGAGGGGGATTTGCAGATTAATGTGGAAGATGAGGAAGCCTTTGTGCTGCCCCCTGCTGGG GAGACGGACCAGGATGGCCAGGCTCCAGACCTGCAGCGAGTTCACAAGCGGATCCAGGACATCGTGGGAG TGCTGCGGGATTTCGGAGCTCAGCGAGAAGAGGGCCGGTCTCGAGCTGAGTATCTAAGTCGGCTTCAGAA GGACCTGGCCACTTACTATTCTTATGGAGACTTCCTGCTGAGCAAGCTCATGGAGCTGTTCCCTCTGTCT GAGTTGATCGAGTTCTTAGAAGCTAATGAGGTGCCCCGGCCAATCACCCTTCGGACCAACACCTTGAAAA CCCGTCGCCGAGACCTTGCTCAGCTTCTGATCAATCGTGGGGTTAATCTGGATCCACTGGGGAAGTGGTC AAAGTCTGGACTTGTGGTATATGATTCTTCAGTGCCTATTGGCGCTACCCCTGAGTACCTCGCTGGACAC TATATGCTGCAGGGAGCTTCCAGTATGTTGCCCGTCATGGCCCTGGCACCTCAGGAGCATGAGCGGATCT TAGACATGTGCTGTGCTCCTGGGGGGAAGACCAGCTACATAGCTCAGCTGATGAAGAACACAGGAGTGAT CCTTGCCAACGATGCCAATGCAGACCGGCTCAAGAGCGTGGTGGGCAACCTGCACCGCTTGGGAGTCACC AACACCATCATCAGCCACTATGATGGGCGCCAGTTCCCCAAGGTGGTAGGGGGCTTTGATCGAGTGCTAC TGGATGCTCCTTGTAGTGGCACAGGAGTCATCTCCAAAGACCCTGCTGTGAAGACGAACAAGGATGAGAA AGACATCCAGCGCTGTGCCCACCTTCAGAAGGAACTGCTGCTCAGTGCTATCGACTCAGTGAACGCTGCC TCCAAGACCGGAGGCTACCTGGTCTACTGCACCTGTTCCATTACGGTGGAAGAGAACGAGTGGGTGGTAG ACTATGCCTTGAAGAAGAGGAATGTGCGGCTGGTGCCCACCGGCCTGGACTTCGGCCAGGAAGGTTTTAC CCGCTTCCAAGCAAGGCGCTTTCACCCCACTCTGCGTTCCACCCGGCGCTTCTACCCTCACACTCACAAT ATGGACGGTTTCTTTATCGCCAAGTTCAAGAAATTTTCCAATTCTATTCCCCAGCCCCATGCAGGAAACT CGGCAGCAGCTACCCCTACAGAGCCTGACCTGAAGGATCAGGTCACCCCAAAGTCTGAGAACGGTAGTCA GCCCACCAAGAAAGCCCGTGGGGCTGTGAAGGCAAAGCAGCAGTTGCTGAGACAGCCACATTCCAAGAAG CCCTTCCAGAAGTTGAACGGCATCGCCAAAGGGCCAGGCTTGTCCACTGAACCTTCTGTCCCAGATGCCC AAGTGTCTACCAGGCCCAGTCAGTCTGCTGGGAATGCTGACGTGAACAGCAAACGAAAGAGGTCTGAGAA GCTGAAGCAGCGGGGACCTAAGTGGAAGCCCTCTAAGGAGGCTGCTGTCCCAAAGCCGAGCGCCCCTTCC AGGGTTGAGGACTCGGGAACACCTGTGCCGACTCCCTCTGAAATCCGTGCTGCCCCAAGGCCTAAGGACT GTGCTCCATCCCTTGGGAAAGCCAAAAAGAAACAGAAAGGAAAGCAGCAGTTAGCACAGCAGCCTGCTAA TGGAGCTGCTCCCCTGAAAGAAGATGCTGTTTCCAAGGGACCCTCAGCCCCCTTTGTGTCTCCCCACAGT TCCACCAGGCCCCCACCAGCAAAAAGGAGGAAATCAATGACAAAGGGCAACAGCCAGCCTCTGTTGTCTT AAATGGACTTGGAAACTTGACATTGTCTCTGGGTTGGAACTTTTAGATCCTGATGCAAGGCTGGCCTCCC CCTGTGCATACATATGAAATTTAATATACATATTACCACTCCTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3592148 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3592148 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19478 same embryo
	EMAGE:19477 same embryo
	EMAGE:19475 same embryo
	EMAGE:19480 same embryo
	EMAGE:19476 same embryo
	EurExpress:euxassay_004863 same experiment
	MGI:4826750 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source