EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19502

Dnajb2 DnaJ (Hsp40) homolog, subfamily B, member 2 ( MGI:1928739)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005046. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005046_01	euxassay_005046_02	euxassay_005046_03	euxassay_005046_04

euxassay_005046_05	euxassay_005046_06	euxassay_005046_07	euxassay_005046_08	euxassay_005046_09

euxassay_005046_10	euxassay_005046_11	euxassay_005046_12	euxassay_005046_13	euxassay_005046_14

euxassay_005046_15	euxassay_005046_16	euxassay_005046_17	euxassay_005046_18	euxassay_005046_19

euxassay_005046_20	euxassay_005046_21	euxassay_005046_22	euxassay_005046_23	euxassay_005046_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19502_wholemount_strong.wlz

19502_wholemount_moderate.wlz

19502_wholemount_weak.wlz

19502_wholemount_possible.wlz

19502_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19502_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 04 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6895
Entity Detected:	Dnajb2, DnaJ (Hsp40) homolog, subfamily B, member 2 ( MGI:1928739)
Sequence:	sense strand is shown >T6895 GCTGACGCATGCTTGCGCACACAACCCAGCCTGGCGTGTCGTACGCAGTGGCCGCGAGCCGGGCAGCGGC CTGGAGGAGGGACGTCTGACGCTGAGTTGCCATGGCATCCTACTACGAGATTCTAGACGTACCGCGGAGT GCGTTCCCTGATGACATCAAGAAGGCGTACCGAAAGAAGGCTCTGCAGTGGCACCCAGACAAGAACCCGG ATAATAAAGAATTTGCTGAAAAAAAATTTAAGGAGGTGGCAGAGGCCTATGAAGTACTATCTGACAAGCA CAAACGGGAGATCTATGACCGCTATGGCCGGGAAGGGCTGACCGGGGCAGGAAGTGGTCCTTCTCGATCG GAAACTGGTGGTGCGGGGCCTGGCTTCACATTCACCTTCCGTAGCCCCGAGGAAGTCTTCCGGGAGTTCT TCGGGAGCGGAGACCCTTTTTCAGAGCTCTTTGATGACTTGGGTGTCTTCTCGGAGCTTCAGAACCAGGG TCCCCGACTCACGGGCCCTTTCTTCACTTTCTCTTCTTCCTTTCCTGCCAACTCCGATTTCTCCTCCTCA TCTTTCTCCTTCAGCCCGGGGGCTGGTGCTTTCCGCTCCGTTTCTACGTCCACCACCTTTGTCCAAGGCC GCCGCATCACCACACGCAGAATCATGGAGAACGGGCAGGAGCGGGTAGAAGTGGAAGAGGATGGACAACT GAAGTCAGTGTCAATCAATGGTGTCCCAGATGACCTGGCACTAGGCTTGGAGCTGAGCCGTCGTGAGCAG CAACCTTCAGTTGCCCCTGGGCTGGGGGTCATGCAGGTCCGGCCGACCTCTCTCTCTCGTCCCCCTGACC ATGATCTTTCTGAGGATGAGGACCTGCAGCTCGCCATGGCTTACAGCCTGTCAGAGATGGAGGCGGCTGG GCAGAAGCCAGCAGATGTGTTCTGAGCTGGATATCCTGGATACAGAGTTGCTGCAGTTCCAACAGGACAG CGCCCTCCCCAGTGTGCTAGGAGGGAACACTACATTCCTCTCCCTCATTTATGCTGAGTGTAGAGCTGGG GGTTGGGGTGGTGGGTGGAGACTGAGTGGGAAGCACCTATAGTCTTAGCCTCTGAACTCTCATCCATGGG TTCTTAGCGCTGTCTTTTGCGGACTACAAGTCCCAGAATGCAATGCACCCTGGACTTGGCTCTTGTAACA CTTATTCCAGGGGAAGAGCTGCTCAGTTACAAAGCTGAGTTACTTGGGATATGTAGTTCCAACCCCGGCC AGTCAGTCACTCTCTGAATGGGGAGATCTTGTTTTTCATTTTGTCCCTGGCAGGGGAGGTGAGAATTGTA GATGACCAGAACTTTAGTTCCTATGGCACTTCTTGCTAGACTCGGGTTGAAGTGAGCAGATTCCTGTAGC ATCTGAGAGAGTTTGTTTTCCCTTGGGCAACCTGGGGGGTCCGAGGAGTAAGCTAATGGGGCAGTGGGCT GTGTAGGTAGGGCACGGTCTTGGATGGATGCGGGCTGGCAGAACCTGCGTGGGAAAGGAGGCTGCCTTGG CTGGAGTGTGTATGGCAAGGACTGGCATGCAGAGGGAGCGTTGGACATATTGCTTGAATGGCTTTCTTTG ACCTCTGACCTTGCTGCCCATTCTTTCTTATCATCAATTCATCCTCCATGAGCCACCTGTCCTCCTGCCT GGGGAGCCCAAAGCCGCATGGGGATTGGTGGAGCAGGCTATGGCCGGCCTCTGTTAACACTGAGCCTTTG AGACCCATCAAAGCAAGATGAGCTGAGCTCAGATTGAGGGGAAACGGAGGCAAATAAAGCTGAGTTTTGA GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3968468 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3968468 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19499 same embryo
	EMAGE:19501 same embryo
	EMAGE:19503 same embryo
	EMAGE:19500 same embryo
	EMAGE:19504 same embryo
	EurExpress:euxassay_005046 same experiment
	MGI:4824345 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source