EMAGE mouse gene expression spatial database

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EMAGE:19557

Rnf181 ring finger protein 181 ( MGI:1913760)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002062. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002062_01	euxassay_002062_02	euxassay_002062_03	euxassay_002062_04

euxassay_002062_05	euxassay_002062_06	euxassay_002062_07	euxassay_002062_08	euxassay_002062_09

euxassay_002062_10	euxassay_002062_11	euxassay_002062_12	euxassay_002062_13	euxassay_002062_14

euxassay_002062_15	euxassay_002062_16	euxassay_002062_17	euxassay_002062_18	euxassay_002062_19

euxassay_002062_20	euxassay_002062_21	euxassay_002062_22	euxassay_002062_23	euxassay_002062_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19557_wholemount_strong.wlz

19557_wholemount_moderate.wlz

19557_wholemount_weak.wlz

19557_wholemount_possible.wlz

19557_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19557_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

strong

homogeneous

strong expression: see section 10 11 17 18 19

thymus primordium

moderate

homogeneous

moderate expression: see section 10 11 12 13 14

brain

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

weak

regional

weak expression: see section 05 20 21

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

spinal cord

weak

homogeneous

weak expression: see section 09 10 11 12 13 14 15 16 17 18 19 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 15 16 17 18 19

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 07 08 09 10 11 12 13 14 15 weak expression: see section 06 16 17 18 19 20 21 22 23 24

testis

strong

homogeneous

strong expression: see section 08 09 10 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5471
Entity Detected:	Rnf181, ring finger protein 181 ( MGI:1913760)
Sequence:	sense strand is shown >T5471 GCGGCAGCCCAGGAGCCATGGCGTCTTATTTTGATGAGCACGACTGCGAGCCGTTGAACCCTGAGCGTGA GGCCCGCAACAATATGTTGCTGGAACTCGCGAGAAGAGTGCGCGGGGCTTGGAGCTGGGCCCCGGGCGGC AGATCACTCTTTAATAGGATGGACTTTGAGGACCTGGGATTGGTAGATTGGGAACACCACCTTCCCCCAC CAGCTGCCAAGGCTGTGGTGGAGAGCCTCCCTAGAACAGTCATCAGTAGCGCCAAGGCTGATCTCAAGTG CCCCGTGTGCCTTTTGGAATTTGAGGCGGAGGAGACTGTGATTGAGATGCCCTGCCATCATCTCTTCCAT TCCAACTGCATTCTGCCCTGGCTAAGTAAGACAAATTCCTGCCCTCTGTGCCGCCATGAGCTGCCCACTG ATGATGACAGCTATGAAGAGCACAAGAAAGACAAGGCTCGCAGGCAGCAGCAGCAGCACCGCCTGGAGAA CCTCCACGGAGCCATGTACACGTGAGGCAGCTGCCAGGGCTGAGCGCTCAGCCTCTCTGATACCTTCCCT CTGCACTCTGAATCATTAAAGGTTTCTTTACCCACCCTGCAGCTGCACGGCTCACCAGCCGAAGCAGGGG CTCCTTCCGAATCCTCCACCCTGCTGCTAGCCAGAAAGATGTCAAAGGTGTCAAATGTCCGAAGGTGCCA GGCTGCAATTCCCTCTGCCAGCCTCTAGACTCTGGGTGGTGGGGCCCTAGAACAACCAGGGCTTGGGTCT GAGTGTTCTTTTAGAAGTCAAACTGTGTCAGAGCCTGGCGCTTTGTAGCTGTTTAAAACTGATAAATTAA CTTGCACCACCCCACTCCGGAAATCTCTAGTTAACCTTGGAACCTTTAGTTAACCGTGGAATCCTGGGGG TGTTCTTTATTTCCACATAGAAAACTTTTAACATTGTAAAAGTTGCTCTCTGTATCCCACTCCTGTAGGC GCCATTATCAAGGAATCAAAGGTCAAGCCAGCCCAAGTAGGGCAAGGCTGTTTAGAGACTCTTCAAAGGG GGACAGTCCTTGTGGAAGTTTTGAAAGTGGGTTTCATTCACTATGTGACCTTGGCTGTCTTGGAACCCAC TATATAGACCAGGCTCGCCTTGTACTTAGGACATCCTTCTGCTCTGTCTTCCCAGTGTTGGGATTACTGG TATGTACACACCCTTACGATGTATCTGAATGGCATCTGTCACTCTTAGTTTCTGGCAACCTCAAGAATCT TTCCTGGGGGAAAGGACTTCTGTCCATCCCTGCCCACTGGATCGGACCCTCCGTGATTCTCCAGAACACA CTGCAGACGTGCAAATTAGAATTCTTTTAATAGATATAAAAAAGTACTAAAATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5374213 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5374213 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19556 same embryo
	EMAGE:19560 same embryo
	EMAGE:19558 same embryo
	EMAGE:19559 same embryo
	EMAGE:19555 same embryo
	EurExpress:euxassay_002062 same experiment
	MGI:4827768 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source