EMAGE mouse gene expression spatial database

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EMAGE:19572

Rhox5 reproductive homeobox 5 ( MGI:97538)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004997. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004997_01	euxassay_004997_02	euxassay_004997_03	euxassay_004997_04

euxassay_004997_05	euxassay_004997_06	euxassay_004997_07	euxassay_004997_08	euxassay_004997_09

euxassay_004997_10	euxassay_004997_11	euxassay_004997_12	euxassay_004997_13	euxassay_004997_14

euxassay_004997_15	euxassay_004997_16	euxassay_004997_17	euxassay_004997_18	euxassay_004997_19

euxassay_004997_20	euxassay_004997_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19572_wholemount_strong.wlz

19572_wholemount_moderate.wlz

19572_wholemount_weak.wlz

19572_wholemount_possible.wlz

19572_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19572_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 weak expression: see section 19 20

heart ventricle

strong

spotted

strong expression: see section 05 06 07 08 09 10 11 12 13

liver

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

ovary

strong

regional

strong expression: see section 03 04 05 15 16

left lung

strong

spotted

strong expression: see section 02 03 04 05 06 07 08 09 10

right lung

strong

spotted

strong expression: see section 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8035
Entity Detected:	Rhox5, reproductive homeobox 5 ( MGI:97538)
Sequence:	sense strand is shown >T8035 GACAAGAGGTAATCCCAGAAGTCGCCATACTTTGGAGAGAGAAGAGCCAAACAGCCATCTCCCTGCACAG TCCTTCAAGCTCACCTCCTGCCTTCCGTGGACAAGAGGAAGCACAAAGAATCATCCAGGTATGGAAGCTG AGGGTTCCAGCCGCAAGGTCACCAGGCTACTCCGCCTGGGAGTCAAGGAAGACTCGGAAGAACAGCATGA TGTGAAAGCAGAGGCTTTCTTCCAGGCTGGAGAGGGGAGAGATGAGCAAGGTGCACAGGGCCAGCCTGGA GTGGGAGCGGTGGGAACAGAAGGCGAAGGAGAAGAATTAAATGGAGGAAAAGGCCACTTTGGTCCTGGTA CTCCTGGTCCTATGGGTGATGGGGACAAGGATAGTGGCACCAGGGCTGGTGGTGTGGAGCAGGAACAAAA TGAGCCAGTTGCTGAGGGCACTGAGAGCCAGGAGAATGGAAATCCTGGGGGTAGGCAGATGCCCCTCCAG GGCTCTAGGTTCGCCCAGCATCGACTGAGGGAACTGGAGTCCATTTTGCAGCGCACTAATTCCTTTGATG TCCCAAGGGAGGATCTTGATAGACTGATGGATGCCTGTGTGTCCAGAGTGCAGAATTGGTTTAAGATCAG GAGGGCTGCGGCCAGAAGAAACAGGAGGAGGGCAACACCAGTCCCTGAACATTTTAGAGGAACATTCGAG TGTCCTGCTTGTCGTGGAGTGAGATGGGGAGAAAGATGCCCTTTTGCGACACCGAGATTTTGATTTGATC ACATATGCCGGCTATGACAGCCCTTACTTTTCAAGAATTCAGCAATAAAGAGGTGGATTCCCAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30022528 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30022528 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19568 same embryo
	EMAGE:19571 same embryo
	EMAGE:19567 same embryo
	EMAGE:19570 same embryo
	EMAGE:19569 same embryo
	EurExpress:euxassay_004997 same experiment
	MGI:4827737 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source