EMAGE mouse gene expression spatial database

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EMAGE:19645

Galnt12 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 12 ( MGI:2444664)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005150. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005150_01	euxassay_005150_02	euxassay_005150_03	euxassay_005150_04

euxassay_005150_05	euxassay_005150_06	euxassay_005150_07	euxassay_005150_08	euxassay_005150_09

euxassay_005150_10	euxassay_005150_11	euxassay_005150_12	euxassay_005150_13	euxassay_005150_14

euxassay_005150_15	euxassay_005150_16	euxassay_005150_17	euxassay_005150_18	euxassay_005150_19

euxassay_005150_20	euxassay_005150_21	euxassay_005150_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19645_wholemount_strong.wlz

19645_wholemount_moderate.wlz

19645_wholemount_weak.wlz

19645_wholemount_possible.wlz

19645_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19645_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 10 11 12 13 14

submandibular gland primordium

weak

regional

weak expression: see section 15 16 17

thyroid gland

weak

regional

weak expression: see section 09 10 13 14

diencephalon roof plate

strong

regional

strong expression: see section 13 14

midbrain ventricular layer

strong

regional

strong expression: see section 11 12

stomach

weak

regional

weak expression: see section 05 06 07 08 09

hindgut

weak

regional

weak expression: see section 12 13

rectum

strong

regional

strong expression: see section 11

midgut

weak

regional

weak expression: see section 13 14 17

tail

weak

regional

weak expression: see section 09 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8603
Entity Detected:	Galnt12, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 12 ( MGI:2444664)
Sequence:	sense strand is shown >T8603 CCCGAGGCCGCGGGGTGCAGCTAGCGGGTGCATGTGGGGGCGTGCGGTGAGGAGGCGCTGCCCGCGGGGG CTGCGGCGCGGCCGGGAGGCGTTGCTGGCACTGCTGGCCCTGGCAGGGCTGGGCGCGCTGCTGCGGGCTC GAAGCAGGTCGGGGACGGTGGACCCGGGACCCCCGCGGACTCCTCTCCCCGGGCGTCACGAGCCAGTGCT GCCCAGGCCGCCGCTGCCCGCCGATGCTCTGGGAGCGCATGGGGAGGCGGTGCGGCTGCAGCTGCAGGGC GAGGAGCTGCGGCTGCAGGAGGAGAGCGTGAAGCAGCACCAGATCAACATCTATCTGAGCGACCGTATCT CGCTGCACCGCCGACTGCCCGAGCGCTGGAACCCTCTGTGCAGAGAGGTGAAATACGATTATGATAACCT GCCCAAGACTTCTGTTGTCATAGCATTTTATAATGAAGCCTGGTCCACACTCCTTCGGACAGTTTACAGT GTTCTTGAGACCTCCCCTGACATCCTGCTGGAGGAGGTCATTCTGGTAGATGACTACAGCGACAGAGAGC ACCTAAAGGAACGCTTGGCCAACGAGCTGTCACAGCTCCCCAAGGTGCGCCTGATCCGTGCTAGCAGGAG AGAGGGCCTAGTGCGAGCCCGGCTACTGGGAGCCTCTGCGGCCAGGGGCGAAGTGCTGACCTTCCTAGAC TGTCACTGTGAATGTCATGAGGGGTGGCTGGAGCCTCTGCTGCAGAGGATCCACGAGAAGGAGTCCGCAG TCGTGTGCCCTGTTATCGATGTGATTGACTGGAACACCTTTGAGTACCTGGGCAACTCGGGAGAGCCGCA GATTGGCGGCTTTGACTGGCGGCTGGTATTCACGTGGCACGTGGTTCCCCAGCGGGAGCGGCAGTCGATG CGGTCGCCGATCGATGTTATCAGGTCTCCAACTATGGCTGGGGGCCTGTTTGCTGTGAGTAAGAGATATT TTGATTATCTGGGGTCTTACGATACAGGAATGGAAGTCTGGGGAGGAGAAAACCTTGAGTTCTCCTTTAG GATCTGGCAGTGTGGTGGCACTCTGGAAACGCACCCGTGCTCCCATGTGGGCCACGTCTTCCCTAAGCAA GCTCCCTATTCCCGTAGCAAGGCCCTGGCCAACAGTGTCCGAGCTGCAGAAGTGTGGATGGATGAATTTA AAGAACTCTACTACCACCGCAATCCCCAGGCCCGCCTGGAACCCTTTGGGGACGTGACAGAGAGGAAGAA GCTTCGGGCTAAGCTCCAGTGTAAAGACTTCAAGTGGTTCTTAGATACAGTGTACCCAGAATTGCACGTG CCAGAGGACAGGCCTGGCTTCTTTGGGATGCTCCAGAACAGAGGCCTAAGAGGGTACTGCCTTGACTACA ATCCTCCCAATGAAAACCATGTTGAAGGCCACCAGGTCCTTCTGTACCTCTGCCATGGGATGGGTCAGAA CCAGTTTTTCGAGTATACGACCCGGAAAGAAATACGCTATAACACCCGCCAGCCCGAAGCCTGCATCACC GTGGAGGACGGGAAGGATACCCTTGTCATGGATCTCTGCCGAGAGACCGTCCCAGAGAACCAGGAGTTCA TCCTACAGGAGGATGGCACTTTAGTTCACAAGCACAGCAGGAAATGTGTGGAGGCCACAGAGAAGGTGTT AGACAACGGCTTTGCACCATACTTACGGGACTGTACCAACTCAGATAACCAGAGGTGGTTCTTCAAGGAG CGCATGTCGTAGTGCATCGCCTTGCCAAGGAAGGAGCCCTCCACGGCTGTGGACTGTGTGCCCAATGAGA TGGACAGCGTACACGGTTGTCATTCGTGAATGTGCTGGGTGTCACAAGAGTGTGAATAAGCTTTGTACAG ATTTTGAAAACTTTAAAAATGTCTCCTGGCATCCTGCTTTCTGAGGGCAATCCTAAGATGTTGCCTTTGG TATTTGGAAAGGATATTTTCCTATCAAGATGTGCATTTTACAATGGTGCCTTTTATTCTCATTAGCAAAA AAGGTAAATACTTTATTTTGATACAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5716909 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5716909 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19644 same embryo
	EMAGE:19647 same embryo
	EMAGE:19642 same embryo
	EMAGE:19643 same embryo
	EMAGE:19646 same embryo
	EurExpress:euxassay_005150 same experiment
	MGI:4824981 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source