EMAGE mouse gene expression spatial database

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EMAGE:19715

Mdm2 transformed mouse 3T3 cell double minute 2 ( MGI:96952)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006190. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006190_01	euxassay_006190_02	euxassay_006190_03	euxassay_006190_04

euxassay_006190_05	euxassay_006190_06	euxassay_006190_07	euxassay_006190_08	euxassay_006190_09

euxassay_006190_10	euxassay_006190_11	euxassay_006190_12	euxassay_006190_13	euxassay_006190_14

euxassay_006190_15	euxassay_006190_16	euxassay_006190_17	euxassay_006190_18	euxassay_006190_19

euxassay_006190_20	euxassay_006190_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19715_wholemount_strong.wlz

19715_wholemount_moderate.wlz

19715_wholemount_weak.wlz

19715_wholemount_possible.wlz

19715_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19715_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8897
Entity Detected:	Mdm2, transformed mouse 3T3 cell double minute 2 ( MGI:96952)
Sequence:	sense strand is shown >T8897 GGCCGGTGAGGAGCCGCCGCCTTCTCGTCGCTCGAGCTCTGGAGCGACCATGGTCGCTCAGGCCCCGGCC GCGGGGCCTCCGCGCTCCCCGTGAAGGGTCGGAAGATGCGCGGGAAGTAGCAGCCGTCTGCTGGGCGAGC GGGAGACCGACCGGACACCCCTGGGGGACCCTCTCGGATCACCGCGCTTCTCCTGCGGGCCTCCAGGCCA ATGTGCAATACCAACATGTCTGTGTCTACCGAGGGTGCTGCAAGCACCTCACAGATTCCAGCTTCGGAAC AAGAGACTCTGGTTAGACCAAAACCATTGCTTTTGAAGTTGTTAAAGTCCGTTGGAGCGCAAAACGACAC TTACACTATGAAAGAGATTATATTTTATATTGGCCAGTATATTATGACTAAGAGGTTATATGACGAGAAG CAGCAGCACATTGTGTATTGTTCAAATGATCTCCTAGGAGATGTGTTTGGAGTCCCGAGTTTCTCTGTGA AGGAGCACAGGAAAATATATGCAATGATCTACAGAAATTTAGTGGCTGTAAGTCAGCAAGACTCTGGCAC ATCGCTGAGTGAGAGCAGACGTCAGCCTGAAGGTGGGAGTGATCTGAAGGATCCTTTGCAAGCGCCACCA GAAGAGAAACCTTCATCTTCTGATTTAATTTCTAGACTGTCTACCTCATCTAGAAGGAGATCCATTAGTG AGACAGAAGAGAACACAGATGAGCTACCTGGGGAGCGGCACCGGAAGCGCCGCAGGTCCCTGTCCTTTGA TCCGAGCCTGGGTCTGTGTGAGCTGAGGGAGATGTGCAGCGGCGGCAGCAGCAGCAGTAGCAGCAGCAGC AGCGAGTCCACAGAGACGCCCTCGCATCAGGATCTTGACGATGGCGTAAGTGAGCATTCTGGTGATTGCC TGGATCAGGATTCAGTTTCTGATCAGTTTAGCGTGGAATTTGAAGTTGAGTCTCTGGACTCGGAAGATTA CAGCCTGAGTGACGAAGGGCACGAGCTCTCAGATGAGGATGATGAGGTCTATCGGGTCACAGTCTATCAG ACAGGAGAAAGCGATACAGACTCTTTTGAAGGAGATCCTGAGATTTCCTTAGCTGACTATTGGAAGTGTA CCTCATGCAATGAAATGAATCCTCCCCTTCCATCACACTGCAAAAGATGCTGGACCCTTCGTGAGAACTG GCTTCCAGACGATAAGGGGAAAGATAAAGTGGAAATCTCTGAAAAAGCCAAACTGGAAAACTCAGCTCAG GCAGAAGAAGGCTTGGATGTGCCTGATGGCAAAAAGCTGACAGAGAATGATGCTAAAGAGCCATGTGCTG AGGAGGACAGCGAGGAGAAGGCCGAACAGACGCCCCTGTCCCAGGAGAGTGACGACTATTCCCAACCATC GACTTCCAGCAGCATTGTTTATAGCAGCCAAGAAAGCGTGAAAGAGTTGAAGGAGGAAACGCAGGACAAA GACGAGAGTGTGGAATCTAGCTTCTCCCTGAATGCCATCGAACCATGTGTGATCTGCCAGGGGCGGCCTA AAAATGGCTGCATTGTTCACGGCAAGACTGGACACCTCATGTCATGTTTCACGTGTGCAAAGAAGCTAAA AAAAAGAAACAAGCCCTGCCCAGTGTGCAGACAGCCAATCCAAATGATTGTGCTAACTTACTTCAACTAG CTGACCTGCTCACAAAAATAGAATTTTATATTTCTAACTATATGACCCCCAAATTAGACAACATGGGTAT TATTTTTATACATTAAAGCCAGAAAACTGTCTTAGTCCACATAAAATTCACTTATAATTTATCCTGGAGA GTAAATATGGTGAATATTTTCTTCCTTTTTAGGGAAATTTCACTTGTTTATTTTATATTTGTGTTTTAAG TAATTTGCATTGGCTGTTTATATTTTCCTTATATTTTAAATAATCTCCGCTTGGAAGGACTTTGGAAGTG TATGTGAGAAGTCCTTTCCATCTCCTGCAGATGATGGTGGACCTTCCTCATCAAGGGCTACAGAAGTACT TGATTTCTGTTTTTTTTGTTAATAATAAGAACATTTAATTTATTTAGTGTCTTTCATGTAAAGAGTTAAA GACTATGTGAAGGATTGTATATTTAAGTTATTGAAATTCTGAAACTGTAGTAATCTAAAATGTGTGAGTT GTGGGCTGCAGAGAAGACTCAGCCAGTAAAGGCACCTGCTGTGTACACCCACTGACACACATTTGATCCT TGGAACCCCCAGGAAGAGTGAACCAGTTCCACAAAGTTGTTCCCTGATCTTCAAATGGATGCACGCATGC ACGCACACACACGCGCGCGTGCGCACACACACACACACACACACACACACACACACACACACACACACAC ACACAGTTTTAAAGGCATGAATTGCATCTGGTGGTATGTAAGTGAAAACACACGCCTTATTTTCCAGCAT TTTCAGCTTTTTGTCATAGGGGTGTGGCACAAGTGTTGCAGTTTGTCCCAGGTTGAAAAGCCTGAGGCTG GTAGAAGCGCCTTTTTGCCTCAGCTCCGTGGTTCCTGGTGGTTGCCTATGTTTCAGGCCTGTACTTAGGC TAGGTTTAGAAACCAGCCCATTCAGAAAGACTGAATCAGAACATGGATAAAGTGAACTCATTCTAAGATG ACTCGTCTATCCATGTAGATTAATCTCCTGGTTCATAATAGGCCTCTTCCCTTTGATTGAAGGGTCACGT CTAAGTATAGAAAACATAAAACTGTAAGGTAGAGGAAGCGAAGGATAGCTTTGTATTAATGTTGCGTTAA AGCTTCAGAGACAAGAACAAGAACACTCCTCCCACGTGACAGCATTTGAATAGGAGGCGGTGGGTGCGGC AGCCTGGGCAGCTTCAGTCCCGATTTACAATAAAGTACCTTGTGTGTTATTAGTTCTTAAATGTTTATTT AGAAATGGCATTGATGTTATTTATTTGCAAATAAATGGTTTATTGAAGAATTGTGAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:6415937 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6415937 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19716 same embryo
	EMAGE:19711 same embryo
	EMAGE:19714 same embryo
	EMAGE:19712 same embryo
	EMAGE:19713 same embryo
	EurExpress:euxassay_006190 same experiment
	MGI:4826143 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source