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EMAGE:19754

Asb4 ankyrin repeat and SOCS box-containing 4 ( MGI:1929751)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19754 EMAGE:19754 EMAGE:19754 EMAGE:19754 EMAGE:19754
"Pseudo-wholemount" of euxassay_006290. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006290_01 euxassay_006290_02 euxassay_006290_03 euxassay_006290_04
EMAGE:19754 EMAGE:19754 EMAGE:19754 EMAGE:19754 EMAGE:19754
euxassay_006290_05 euxassay_006290_06 euxassay_006290_07 euxassay_006290_08 euxassay_006290_09
EMAGE:19754 EMAGE:19754 EMAGE:19754 EMAGE:19754 EMAGE:19754
euxassay_006290_10 euxassay_006290_11 euxassay_006290_12 euxassay_006290_13 euxassay_006290_14
EMAGE:19754 EMAGE:19754 EMAGE:19754 EMAGE:19754 EMAGE:19754
euxassay_006290_15 euxassay_006290_16 euxassay_006290_17 euxassay_006290_18 euxassay_006290_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19754Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19754_wholemount_strong.wlz
19754_wholemount_moderate.wlz
19754_wholemount_weak.wlz
19754_wholemount_possible.wlz
19754_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19754_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 04 15 16 17 18 weak expression: see section 01 02 03
diaphragm
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 12 13 14 15 16 17
forearm rest of mesenchyme
moderate moderate
regionalmoderate expression: see section 01 19
upper arm rest of mesenchyme
moderate moderate
regionalmoderate expression: see section 01 19
foot
moderate moderate
regionalmoderate expression: see section 03
hindlimb digit 1 mesenchyme
moderate moderate
regionalmoderate expression: see section 04 16
hindlimb digit 2 mesenchyme
moderate moderate
regionalmoderate expression: see section 04 16
hindlimb digit 2 phalanx
weak weak
regionalweak expression: see section 05
hindlimb digit 3 mesenchyme
moderate moderate
regionalmoderate expression: see section 04 16
hindlimb digit 3 phalanx
weak weak
regionalweak expression: see section 05
hindlimb digit 4 mesenchyme
moderate moderate
regionalmoderate expression: see section 04 16
hindlimb digit 4 phalanx
weak weak
regionalweak expression: see section 05
hindlimb digit 5 mesenchyme
moderate moderate
regionalmoderate expression: see section 04 16
lower leg rest of mesenchyme
moderate moderate
regionalmoderate expression: see section 01 17 18 19
vertebral axis musculature
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 13 14 15 16 17 18 19
adrenal gland
moderate moderate
regionalmoderate expression: see section 04 05 06 12 14 weak expression: see section 13
diencephalon lateral wall mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 12 13 14 15
diencephalon lateral wall ventricular layer
moderate moderate
regionalmoderate expression: see section 11 12
lateral ventricle
moderate moderate
regionalmoderate expression: see section 06
telencephalon mantle layer
moderate moderate
regionalmoderate expression: see section 03 04 07 08 09 10 11 12 13 14 15 16 17 weak expression: see section 05
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 14 15 16 weak expression: see section 04 05 17
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15
medulla oblongata basal plate ventricular layer
moderate moderate
regionalmoderate expression: see section 10
pons mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 12 13 14 15 16
pons ventricular layer
moderate moderate
regionalmoderate expression: see section 10 11
metencephalon part of 4th ventricle choroid plexus
weak weak
regionalweak expression: see section 03 04 05 06 07 08 09 11 12 13 14 15 16
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 05 09 10 11 12 13 14 15 16 weak expression: see section 08
spinal cord floor plate
moderate moderate
single cellmoderate expression: see section 08 09 10
ventral grey horn
moderate moderate
single cellmoderate expression: see section 08 09 10 11 12
viscerocranium
weak weak
regionalExpression in the turbinate bone.
tongue
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14
stomach
weak weak
regionalweak expression: see section 01 02 03
hindgut
moderate moderate
regionalmoderate expression: see section 09
midgut
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 weak expression: see section 01 02
bladder
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11
metanephros
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 11 12 13 14 15
axial skeleton
moderate moderate
regionalmoderate expression: see section 07 08 weak expression: see section 11 12
axial skeleton tail region
moderate moderate
regionalmoderate expression: see section 09 10
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8468
Entity Detected:Asb4, ankyrin repeat and SOCS box-containing 4 ( MGI:1929751)
Sequence:sense strand is shown

>T8468
GCAAAGTGCTACCCCAAAAGGGCTCCGAGGATGGACGGCATCACTGCCCCTATCAGCAAAGCTGGAGCTG
CCAAATTAGTTAAGAAAGATTTCCTGGAGGCTCTAAAAACAAATGACTTCGGAAAACTGAAGGCTATTTT
AATCGAGAGGCAAATAGATGTGGACACAGTGTTTGAAGTTGAAGATGAGAACATGATTTTGGCATCTTAC
AAACAAGGCTATTGGTTGCCCAGTTATAAGCTGAAGTCTTCCTGGGCCACAGGCCTGCATCTGTCAGTTT
TGCTTGGTCATGTGGAGTGCCTGATGGTACTGCTGGACCACAATGCTACCATCAACTGTCGTCCCAACGG
GAAGACCCCTCTGCACGTGGCTTGTGAAATCGCCAACCTCGAGTGCGTGAAGATTCTCTGTGACCGTGGC
GCAAAGCTCAACTGCTACTCCCTGAGTGGGCACACTGCTTTGCACTTCTGCACCACACCGAGCTCCATCC
TCTGTGCCAAGCAGTTGGTGTTGAGAGGTGCAAACGTAAACATGAAAACCAACAACCAGGATGAAGAGAC
ACCCCTGCACACGGCGGCCCACTTCGGCCTCTCTGAGCTGGTTGCCTTCTACGTGGAGAACGGGGCCATC
GTGGATAGCATGAACGCCCACATGGAGACCCCACTGGCCATTGCCACCTACTGGGCCCTGCGCTTCAAGG
AGCAGGAGTACAGCAGGGAACACCACCTCATCTGCCGCACCCTGCTGGACAACAACGCCGAGGTCAACGC
TCGTGATGATGATTTCAAGTCCCCTCTACACAAGGCTGCCTGGAATTGCGACCACGTGCTCATGCACATG
ATGCTGGAGGCAGGAGCTGAGGCCAACCTCATGGATATCAATGGCTGTGCCGCTATCCAGTACGTGCTGA
AGGTCACCTCCGTGCGTCCTGCTGCACAGCCTGAGATCTGCTACCAGCTGCTGCTGAACCACGGGGCTGC
GCGCATCTACCCCCCACAGTTCCACAAGGTGATCCAGGCTTGCCACTCTTGCCCGAAAGCCATTGAAGTT
GTGGTCAATGCCTATGAACACATTCGATGGAACATAAAGTGGAGACGAGCCATTCCTGATGATGACTTGG
AGAAACACTGGGATTTTTACCACTCTCTCTTCAAGGTGTGCTGCAACACCCCCAGAACCCTCATGCACCT
GTCAAGATGTGCCATTCGAAAAGCGCTACACAACAGATGCCACAAAGCAATCCCTATGCTGTCCCTCCCA
CTGCCGTTGAAGAAGTACCTGCTTTTGGAGCCAGAGGGAATCATTTACTAAGTCTTAGGAGACAACAGTT
GCCAATCCTAGATTTCTAAGGGGACTCACTGGGTAGACGCAGTTTGCATGGATAAAATGGTCCTTGGGTT
GGTTATCACACTTTAGGGATTTTAAAACACTTTACAAACACCAAGCCATTAATCCTAGAGTAGTATGCTG
AGGGAAAATAAGCGAGAAATGTTAAAGAATTTTCAGACACAATGGCTACATTTAGAAGGCAGATACTATT
ATAGTAGCTCATGTGACAACATACAGTGACAACTTTTATTAGGTGCCTGATGTAATTTGTAAAATGTTCA
CATATGATTTCATCTCAAAAGGGACCCACATTTTCTTATAACCAATCTCATTCACTACATTATCAGTAAT
ACGTTTACATGATTGTTTCGTTTGCAATGTAGCAGGTTTTGTGAGTGACCGCTTAAGCTTGGTGTCTCCC
ACCAGTGACTATTGAGGAGACATTACTGTTACTATTGAGGAGACATTACTGTTACTAGTTTTAGAAACGC
CTTCTCCCCAAATCAAAGTCATCCACTCCTTTTGGTGACAAATGCACAGGAGTACAGTGGGAACAAGATC
TTTGAGTCTAGATAGAAAAAGAAGGGAAGAGCAGTTTACTTTATAGCCTAGGAATAGACATTTCAACACA
CTGAGCCAGACACTGGAGGCCGCAGATGGACCTCCAGGGTTGAATGTTTAAAAGGGGATCCAAACATACG
AGCAGATGACTGCTGTATGAAGTGATGGGGCAGGAGTACAGGTGCAAACTCATATGTACTGCTTAACTTT
CTGAAGTGTGCTCTGACTTGAAGTGCGTGATTTATTTTATTTTATTTTTTTTTAACCATGAAGACAAAGC
ACGGAGTACTGTTTAGAGGAACCTGCTACTATTGGAGTAAATGTCTTTGAATTTTTCCAGACTATTGAGA
TAGTGTTTTATTTTGTGTGGTTTCCTGATTTTAGTACCTTTCCAGAGTCTGAGAAAAAATATTCTACACT
CCAGCTCTCATGTAGACGAGAAAGAAATTTGAGGAAGTTTTTAGGCAAAAGCAGTTGTAAAGAGAACGTC
TTAATTGATTTTCTTGGTGTTTTCATGGAACAGGTAGACAGTTTAGAGTATTTTAGCTGATAGAAAAGGA
ACTTGCGTTTTAACAGTGGCTCCCTTCAGTTGGAAAAAGAAAGAAAGAAAAAAAGAAAAAAAAAACCTTT
GCATAGAAGGAAATATGCTATAAGACTTATTTAGTATTTGCTAGTACAATCACACTGGTATGCCTCCTGG
AGAACTTTGTTTTCCTCATTTGGTTCTCAGTGATCACTGTTGGATTAGCTTGTTGCCTCTGGGCACAGAC
TTGGTACCAGTTAAAATTGTAAGTAGTGACGTGTAGACACTGTACCGCTGTGGGCAGACTCCTTTCATTT
CTCTGTGGCGTTGCTTCTTGCTTCCTGTCGGATGTTCTCCTGTTGTGGTGACTAGAGATGTTGTCAGACA
TCCCAGTAGTTCCCGATGTAGCTGTGTTTGTAAATCGAGACCCTCTTACTCCCAGTTTTGTGTAGGTGGA
CACGTTCATGTATTTGATGTATCAATTTAACTCAACTGATTTCAAAAAAAAAAATTCTGTATTAGAACAA
GATCTCTAGCCTGAAACGGAATGCTGAATTTGTACAGATTACACATAGAAAGTAGTTTGCTTAACTGAGT
GTTATTTAGAACATATTTCAGAATATTCAAAGGGTTGGGGGCTTCTCTCCATCACCAGGAACATTCTGGC
AGAAAAGTCTGTGCTGGACTGATTTATCACAGGTGTAGTGGGACTGAGAACAAGCAGACAAGGTTTTCCT
CATATAGGGAGCTAGGGATGTTTGTCATAGTGTTGCAATGTTTCCCTTAGTCTAGTGTGGAATATGTAGC
CACTTGTTTTGAATTGCTTGCACATGTGTTTAATGTGATTATTTCTGTACCTTGTCCTTTAATAAAACTT
CAATAAAGATCATAAATAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5703017 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5703017 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19758 same embryo
 EMAGE:19756 same embryo
 EMAGE:19755 same embryo
 EMAGE:19757 same embryo
 EMAGE:19759 same embryo
 EurExpress:euxassay_006290 same experiment
 MGI:4823270 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS