EMAGE mouse gene expression spatial database

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EMAGE:19773

Sipa1l2 signal-induced proliferation-associated 1 like 2 ( MGI:2676970)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006320. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006320_01	euxassay_006320_02	euxassay_006320_03	euxassay_006320_04

euxassay_006320_05	euxassay_006320_06	euxassay_006320_07	euxassay_006320_08	euxassay_006320_09

euxassay_006320_10	euxassay_006320_11	euxassay_006320_12	euxassay_006320_13	euxassay_006320_14

euxassay_006320_15	euxassay_006320_16	euxassay_006320_17	euxassay_006320_18	euxassay_006320_19

euxassay_006320_20	euxassay_006320_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19773_wholemount_strong.wlz

19773_wholemount_moderate.wlz

19773_wholemount_weak.wlz

19773_wholemount_possible.wlz

19773_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19773_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 07 08 09 10 11

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16

telencephalon mantle layer

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 05 13 weak expression: see section 04 10 11 12

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 07 08 09

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 01 05 06 09 13 14 15 16 17 weak expression: see section 02 03 04 10 11 12

pons ventricular layer

moderate

regional

moderate expression: see section 08 09

midbrain mantle layer

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35001
Entity Detected:	Sipa1l2, signal-induced proliferation-associated 1 like 2 ( MGI:2676970)
Sequence:	sense strand is shown >T35001 CATGGACAAGAACTAGAAGGGGCCCCAGAGCTGTCCTTGTGTGTAGATCCCACCAGTGGCAAAGAGTTCA TGGATACACCTGGGGAGCGATCGCCCTCCACACTGACTGGGAAAGTTAACCAGCTAGAACTGATTCTCCG ACAACTGCAGACTGACCTTCGGAAGGAGAAGCAGGACAAGGCGGTGCTGCAGGCTGAGGTCCAGCACCTG AGGCAGGACAACATGCGGCTGCAGGAAGAGTCACAGACAGCCACAGCCCAGCTGCGCAAGTTCACCGAGT GGTTTTTTAGCACCATTGACAAGAAGGCCTGATCAGCTCGCTGCTCCTCACCTTGGGACCACGGGCTGGT CGGCCTGCCTGCCTGCCACCCTGCCTACCCGTGGTCCCTTCCAAGTCAGCAAAGCAGTTTTTACATGTGC TTTCCATCACCTGTAGGTAGATGTCTCACTGTCTGTCTGTGTCTCTACTCACCCCAGCAGGCAGGTCAGG GGCCTGGACTCCCGCATAGCTGCAGCAGAAGATGAATGTAACTGCTGGTGGATGCTGACAGCCTGTAGCC GAGTGGTTTCCCTGACGATGCTCGGGTCTCCTTCCTGTGGGGAGAGAACCTAGGCAGGTGACCTGCAGAG ATGCTAAGCCAGTGGACTATGGCAGGGCTGTAGGGCTGTAAAATAACTATGGAGACGAGAATCAGACATG TACTTTAATGTTAAAGGTGCTCTATTTTTCGGATGTACAGTAGTTTTATTTCCACAGCCGCATTATCATA GCAATAAGAAGGGCACGCAGTAGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 50005. Forward Primer - name:050005_F_cDNA_Sipa1l2, sequence:CATGGACAAGAACTAGAAGGGG; Reverse Primer - name:050005_N_SP6_cDNA_Sipa1l2, sequence:CCTACTGCGTGCCCTTCTTAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19777 same embryo
	EMAGE:19774 same embryo
	EMAGE:19776 same embryo
	EMAGE:19772 same embryo
	EMAGE:19775 same embryo
	EurExpress:euxassay_006320 same experiment
	MGI:4828077 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source