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EMAGE:19817

Wdr44 WD repeat domain 44 ( MGI:1919654)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19817 EMAGE:19817 EMAGE:19817 EMAGE:19817 EMAGE:19817
"Pseudo-wholemount" of euxassay_006374. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006374_01 euxassay_006374_02 euxassay_006374_03 euxassay_006374_04
EMAGE:19817 EMAGE:19817 EMAGE:19817 EMAGE:19817 EMAGE:19817
euxassay_006374_05 euxassay_006374_06 euxassay_006374_07 euxassay_006374_08 euxassay_006374_09
EMAGE:19817 EMAGE:19817 EMAGE:19817 EMAGE:19817 EMAGE:19817
euxassay_006374_10 euxassay_006374_11 euxassay_006374_12 euxassay_006374_13 euxassay_006374_14
EMAGE:19817 EMAGE:19817 EMAGE:19817 EMAGE:19817 EMAGE:19817
euxassay_006374_15 euxassay_006374_16 euxassay_006374_17 euxassay_006374_18 euxassay_006374_19
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euxassay_006374_20 euxassay_006374_21 euxassay_006374_22 euxassay_006374_23 euxassay_006374_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19817Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19817_wholemount_strong.wlz
19817_wholemount_moderate.wlz
19817_wholemount_weak.wlz
19817_wholemount_possible.wlz
19817_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19817_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
tongue muscle
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35071
Entity Detected:Wdr44, WD repeat domain 44 ( MGI:1919654)
Sequence:sense strand is shown

>T35071
CAGTCAGGTTATGGCACATTTCTAGAAGAGAATGCCTTTGCTGTTTTCAACATATAGATTTTGTCACTGC
CATCGCTTTCCATCCAAGAGATGACAGATATTTTCTCAGTGGATCATTGGATGGAAAGCTCCGCCTATGG
AACATACCTGACAAAAAGGTGGCTTTATGGAATGAAGTAGATGGTCAGACAAAGTTGATCACAGCTGCCA
ATTTTTGTCAGAATGGCAAATATGCAGTAATTGGGACATATGATGGTAGATGTATTTTCTATGATACAGA
GCATTTGAAATACCATACACAAATACATGTACGATCTACTAGAGGACGCAACAAGGTTGGAAGAAAAATT
ACTGGCATTGAACCTTTACCTGGAGAAAATAAGATACTGGTAACCTCAAATGACTCCAGAATCAGACTGT
ATGATCTGAGAGACCTATCACTGTCCATGAAGTATAAGGGTTATGTCAATAGCAGCAGCCAGATCAAAGC
AAGTTTCAGTCATGACTTTACTTACCTCGTTAGCGGTTCAGAAGATAAATATGTTTATATCTGGAGTACG
TACCATGACCTAAGCAAGTTT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 88291. Forward Primer - name:088291_F_cDNA_2610034K17Rik, sequence:CAGTCAGGTTATGGCACATTTC; Reverse Primer - name:088291_N_SP6_cDNA_2610034K17Rik, sequence:AAACTTGCTTAGGTCATGGTACG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19815 same embryo
 EMAGE:19818 same embryo
 EMAGE:19819 same embryo
 EMAGE:19816 same embryo
 EurExpress:euxassay_006374 same experiment
 MGI:4829200 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS