EMAGE mouse gene expression spatial database

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EMAGE:19836

Adcyap1r1 adenylate cyclase activating polypeptide 1 receptor 1 ( MGI:108449)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009317. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009317_01	euxassay_009317_02	euxassay_009317_03	euxassay_009317_04

euxassay_009317_05	euxassay_009317_06	euxassay_009317_07	euxassay_009317_08	euxassay_009317_09

euxassay_009317_10	euxassay_009317_11	euxassay_009317_12	euxassay_009317_13	euxassay_009317_14

euxassay_009317_15	euxassay_009317_16	euxassay_009317_17	euxassay_009317_18	euxassay_009317_19

euxassay_009317_20	euxassay_009317_21	euxassay_009317_22	euxassay_009317_23	euxassay_009317_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19836_wholemount_strong.wlz

19836_wholemount_moderate.wlz

19836_wholemount_weak.wlz

19836_wholemount_possible.wlz

19836_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19836_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal medulla

weak

regional

weak expression: see section 06 07 08 09 16 17

submandibular gland primordium mesenchyme

moderate

regional

moderate expression: see section 09 10 17 18 19 20

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 12 13 14

telencephalon ventricular layer

strong

regional

strong expression: see section 03 04 05 06 09 10 15 16 17 18 19 20 moderate expression: see section 02 07 08 12 13 14 21 22 23

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 11 12 13

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

pons ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

midbrain ventricular layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 moderate expression: see section 16

trigeminal v nerve

strong

regional

strong expression: see section 05 06 07 08 09 10 17 18 19 20

spinal cord

moderate

regional

moderate expression: see section 10 11 12 13 14 15

spinal cord ventricular layer

strong

regional

strong expression: see section 11 12 13

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 11 16 17

cervical ganglion

moderate

regional

moderate expression: see section 09 17 18

thoracic ganglion

moderate

regional

moderate expression: see section 11 12 13

heart ventricle

moderate

spotted

moderate expression: see section 08 09 10 11 12 13 17 18 19 weak expression: see section 14 15 16

esophagus

moderate

spotted

moderate expression: see section 12 13 14

tongue

strong

spotted

strong expression: see section 13 15 16 17

stomach

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 weak expression: see section 09

midgut

moderate

spotted

moderate expression: see section 04 05 06 07 08 12 13 14 15 weak expression: see section 09 10 11 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35095
Entity Detected:	Adcyap1r1, adenylate cyclase activating polypeptide 1 receptor 1 ( MGI:108449)
Sequence:	sense strand is shown >T35095 TTAAGAGGAAATGGAGGAGCTGGAAGGTGAACCGTTACTTCACTATGGACTTCAAGCACCGGCATCCATC CCTGGCCAGCAGTGGAGTGAACGGGGGCACCCAGCTGTCCATCCTGAGCAAGAGCAGCTCCCAGCTCCGC ATGTCCAGCCTCCCGGCCGACAACTTGGCCACCTGAGCCCTGTCTCCCTTCTTCTTCTGCACAGGCTGGG GCTGCGGGCCAGTGCCTGCGCATGTTTGTGCCTCTCCCCTCTCCTTGGGCAGGCCCTGGGTAGGAAGCTG GGCTCCTCCTCAAAGGGGAAGAGAGAGATAGGGTATAGGCTGATATTGCCCCTCCTGTTTGGGTCCCATC TACTGTGGTCCACTGAGCCCAATTTGATATGTAAATACACCTCAAATTTGGAAAGTTGCCCCATCTCTCC CCCCCAACCCATGCCCCTGCTCACCTCTGCCAGGTCCCAGCTTGACCTACTGGGTCAAGGCCAGCCTCGG TGATGGCCTGATCCCAGGTACAAGGCCTTGTGAACTGAGTTTGAAACATGCCTGCTTTGGAGAGACTGGG GCAGTGAACACCCCAGCAGCCTTTCAGCAAATTGGCTTTGGACATGGACCCTTCTCAGCCTGTACCAAGT ACTGCAGCTCAGTGTCATGAGCATCCTTTGGAGCAGGTCAACCTGAGGCTCCTTTGGCTTACATCTAAGT TGTCCAGGTGCTCAGCTCCTGTGTGCCTGGATGATGAGAGGGCTCTAGGGCTCTTTCAGTCAGAGACTTA CATTGAGGTGGGGTGAGGGTCAGAGAAAGTTCTGGTGCTTTTCCTGTGCTCTAAGAGCTGAGAGCCAGAA ATGCAGATTCAATTTGGAAGGAGATGGGATAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 71047. Forward Primer - name:071047_F_cDNA_Adcyap1r1, sequence:TTAAGAGGAAATGGAGGAGCTG; Reverse Primer - name:071047_N_SP6_cDNA_Adcyap1r1, sequence:CTATCCCATCTCCTTCCAAAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19837 same embryo
	EMAGE:19840 same embryo
	EMAGE:19835 same embryo
	EMAGE:19839 same embryo
	EMAGE:19838 same embryo
	EurExpress:euxassay_009317 same experiment
	MGI:4823001 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source