EMAGE mouse gene expression spatial database

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EMAGE:19919

Ubr3 ubiquitin protein ligase E3 component n-recognin 3 ( MGI:1861100)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009294. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009294_01	euxassay_009294_02	euxassay_009294_03	euxassay_009294_04

euxassay_009294_05	euxassay_009294_06	euxassay_009294_07	euxassay_009294_08	euxassay_009294_09

euxassay_009294_10	euxassay_009294_11	euxassay_009294_12	euxassay_009294_13	euxassay_009294_14

euxassay_009294_15	euxassay_009294_16	euxassay_009294_17	euxassay_009294_18	euxassay_009294_19

euxassay_009294_20	euxassay_009294_21	euxassay_009294_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19919_wholemount_strong.wlz

19919_wholemount_moderate.wlz

19919_wholemount_weak.wlz

19919_wholemount_possible.wlz

19919_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19919_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 weak expression: see section 05 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 16 17 18 19 20 weak expression: see section 07 08

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 05 06 16

dorsal root ganglion

weak

regional

weak expression: see section 05 06 07 08 09 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35801
Entity Detected:	Ubr3, ubiquitin protein ligase E3 component n-recognin 3 ( MGI:1861100)
Sequence:	sense strand is shown >T35801 AGCGGGTTTTGTAGAAGACATCAGATTAAATCAAGTTCAAATATTCCCTGTGTCCCCAAAGACTTACTGA TGATGTCTGAATTTGTTCTTCCAAGATTCATTTTTTGTCTTATTCAGTACTTAAGAGAAGGCTATAATGA ACCAGCTGATGCACCATCAGAAAAAGATCTGAACAAAGTCCTTCAGCTTTTGGAGCCTCAGATTTCCTTT TTAGAAGACCTGACTAAAATGGGAGGAGCCATGAGGTCTGTTCTTACTCAGGTGTTAACCAACCAGCAGA ACTACAAAGACCTGACTGCGGGTCTTGGAGAAAATGCTTGTGCAAAGAAAAGTCACGAGAAGTACCTTAT AGCTTTGAAGAGCTCCGGACTGACATATCCCGAGGATAAGCTGGTGTATGGGGTCCAGGAACCAGCTGCT GGTACTAGCACTCTGGCAGCTCAAGGTTTTGCAGGTGCTACTGGAACACTGGGGCAGATAGACTCTTCAG ATGAGGAGGACCAGGATGGGAGTCAAGGCTTGGGCAAGAGAAAGAGGGTGAAGCTAAGCAGTGGCACCAA AGATCAATCCATAATGGATGTTTTGAAACATAAAAGTTTTTTAGAAGAACTATTATTTTGGACAATAAAG TATGAGTTCCCACAGAAGATGGTAACTTTCTTGCTCAATATGCTCCCAGATCAAGAGTATAAGGTTGCTT TTACAAAAACATTTGTTCAGCATTATGCTTTTATTATGAAAACACTGAAGAAAAGTCACGAATCAGATAC AATGTCCAACAGAATTGTGCATATTAGTGTTCAGTTGTTCAGCAATGAGGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 70442. Forward Primer - name:070442_F_cDNA_A130030D10Rik, sequence:AGCGGGTTTTGTAGAAGACATC; Reverse Primer - name:070442_N_SP6_cDNA_A130030D10Rik, sequence:TCCTCATTGCTGAACAACTGAAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19918 same embryo
	EurExpress:euxassay_009294 same experiment
	MGI:4829065 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source