EMAGE mouse gene expression spatial database

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EMAGE:19982

Sdk1 sidekick homolog 1 (chicken) ( MGI:2444413)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009453. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009453_01	euxassay_009453_02	euxassay_009453_03	euxassay_009453_04

euxassay_009453_05	euxassay_009453_06	euxassay_009453_07	euxassay_009453_08	euxassay_009453_09

euxassay_009453_10	euxassay_009453_11	euxassay_009453_12	euxassay_009453_13	euxassay_009453_14

euxassay_009453_15	euxassay_009453_16	euxassay_009453_17	euxassay_009453_18	euxassay_009453_19

euxassay_009453_20	euxassay_009453_21	euxassay_009453_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19982_wholemount_strong.wlz

19982_wholemount_moderate.wlz

19982_wholemount_weak.wlz

19982_wholemount_possible.wlz

19982_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19982_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 17 18 19 weak expression: see section 16 20 21

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 09 13 14 weak expression: see section 08 15

neural retina

moderate

regional

moderate expression: see section 01 02 weak expression: see section 03 22

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 14 15 16 17 weak expression: see section 08 09 18

vomeronasal organ

moderate

regional

moderate expression: see section 11 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36996
Entity Detected:	Sdk1, sidekick homolog 1 (chicken) ( MGI:2444413)
Sequence:	sense strand is shown >T36996 AAAATGAAGGTCCTCTTCCTCCCCGAGCCTGTGGTGAAGATCAAGGATCTCACCAGCCACACAAAGTACC TGATCAGCATCTCAGCCTTCAATGCTGCTGGTGATGGGCCCAAGAGTGACCCGTGCCAAGGACGCACACA TCAGGCGGCTCCAGGGCCCCCCAGCTTCTTGGCATTCTCAGAAATAACTTCTACCACACTCAACGTATCC TGGGGAGAGCCATCAGCTGCCAACGGCATCCTACAGGGCTATCGAGTGGTGTATGAGCCCTTAGCACCAG TGCAAGGCGTGAGCAAGGTGGTGACCGTGGACGTGAAAGGCAACTGGCAACGTTGGCTGAAGGTGCGGGA CCTCACCAAGGGAGTGACCTACTTCTTCCGTGTCCAGGCGCGGACCATCGCCTACGGGCCGGAGCTCCAA GCCAATGTCACTGCAGGGCCAGCCGAGGGGTCCCCAGGCTCACCAAGAAATGTCCTTGTCACCAAATCTG CCTCTGAGCTGACCCTTCAGTGGACAGAGGGAAATGCTGGTACCACACCCACTACAGGCTACGTCATAGA AGCCAGGCCATCAGATGAAGGCTTATGGGACATGTTTGCAAAGGACAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 72727. Forward Primer - name:072727_F_cDNA_Sdk1, sequence:AAAATGAAGGTCCTCTTCCTCC; Reverse Primer - name:072727_N_SP6_cDNA_Sdk1, sequence:ATGTCCTTTGCAAACATGTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19980 same embryo
	EMAGE:19981 same embryo
	EMAGE:19979 same embryo
	EMAGE:19978 same embryo
	EurExpress:euxassay_009453 same experiment
	MGI:4827924 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source