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EMAGE:20341

Acox2 acyl-Coenzyme A oxidase 2, branched chain ( MGI:1934852)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20341 EMAGE:20341 EMAGE:20341 EMAGE:20341 EMAGE:20341
"Pseudo-wholemount" of euxassay_012512. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012512_01 euxassay_012512_02 euxassay_012512_03 euxassay_012512_04
EMAGE:20341 EMAGE:20341 EMAGE:20341 EMAGE:20341 EMAGE:20341
euxassay_012512_05 euxassay_012512_06 euxassay_012512_07 euxassay_012512_08 euxassay_012512_09
EMAGE:20341 EMAGE:20341 EMAGE:20341 EMAGE:20341 EMAGE:20341
euxassay_012512_10 euxassay_012512_11 euxassay_012512_12 euxassay_012512_13 euxassay_012512_14
EMAGE:20341 EMAGE:20341 EMAGE:20341 EMAGE:20341 EMAGE:20341
euxassay_012512_15 euxassay_012512_16 euxassay_012512_17 euxassay_012512_18 euxassay_012512_19
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euxassay_012512_20 euxassay_012512_21 euxassay_012512_22 euxassay_012512_23 euxassay_012512_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20341Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20341_wholemount_strong.wlz
20341_wholemount_moderate.wlz
20341_wholemount_weak.wlz
20341_wholemount_possible.wlz
20341_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20341_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
heart ventricle
strong strong
spottedstrong expression: see section 05 06 07 08 09 10 11 12 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31391
Entity Detected:Acox2, acyl-Coenzyme A oxidase 2, branched chain ( MGI:1934852)
Sequence:sense strand is shown

>T31391
GCCTTCATTGTGCCCATCCGGAGCCTAGAGGACCACACCCCACTGCCAGGAATAACAGTTGGGGACATAG
GCCCCAAGATGGGCTTCGAAAACATAGACAATGGCTTCCTGCGACTGAACCACGTGAGGGTTCCCAGAGA
GAACATGCTCAGTCGCTTTGCAGAGGTCTTGCCAGATGGCACCTACCAGAGGCTTGGGACACCACAGAGC
AATTATCTTGGCATGTTGGTGACACGGGTGCAGCTGCTGTATAAAGGATTCCTACCCACCCTCCAGAAGG
CTTGCACCATTGCTGTGCGCTACGCAGTAATCCGCCACCAGTCTCGTCTTCGGCCTAGTGACCCAGAGGC
AAAAATCCTGGAATACCAGACACAGCAGCAGAAACTCCTGCCTCAGCTTGCTGTGAGCTATGCCTTGCAT
TTCATGACCACCAGCCTCTTACAATTCTTCCACAGCTCCTACAGTGATATTCTGAAGAGAGACTTCAGCC
TCCTGCCTGAGCTCCATGCACTGAGCACTGGCATGAAGGCCATGTCTTCAGACTTCTGTGCCCAGGGAAC
AGAGATCTGCCGCAGGGCCTGCGGGGGTCATGGCTACTCAAAGCTGAGCGGCCTACCGACACTGGTCACT
CAAGCGATAGCCTCTTGCACGTATGAGGGTGAGAATACGGTGCTCTACCTGCAAGTGGCCAGGTTTCTGA
TGAAGAGCTACCTGCAGGCTCAAGTGTCCCCAGGATCCATACCACAGAAGCCTCTCCCTCAGTCCGTCAT
GTATCTCGCCACACCAAGACCAGCCAGGTGCCCAGCCCAGACAGCAGCTGACTTCCGCTGCCCAGAAGTC
T
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5052291), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 24477. Forward Primer - name:024477_F_IRAV55-58_J16_Acox2, sequence:GCCTTCATTGTGCCCATC; Reverse Primer - name:024477_R_SP6_IRAV55-58_J16_Acox2, sequence:TAGACTTCTGGGCAGCGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20339 same embryo
 EMAGE:20340 same embryo
 EMAGE:20338 same embryo
 EMAGE:20337 same embryo
 EurExpress:euxassay_012512 same experiment
 MGI:4822931 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS