EMAGE mouse gene expression spatial database

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EMAGE:20513

Rnf186 ring finger protein 186 ( MGI:1914075)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002022. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002022_01	euxassay_002022_02	euxassay_002022_03	euxassay_002022_04

euxassay_002022_05	euxassay_002022_06	euxassay_002022_07	euxassay_002022_08	euxassay_002022_09

euxassay_002022_10	euxassay_002022_11	euxassay_002022_12	euxassay_002022_13	euxassay_002022_14

euxassay_002022_15	euxassay_002022_16	euxassay_002022_17	euxassay_002022_18	euxassay_002022_19

euxassay_002022_20	euxassay_002022_21	euxassay_002022_22	euxassay_002022_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20513_wholemount_strong.wlz

20513_wholemount_moderate.wlz

20513_wholemount_weak.wlz

20513_wholemount_possible.wlz

20513_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20513_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

moderate

regional

moderate expression: see section 12

hindgut

moderate

regional

moderate expression: see section 09 10 11 12

rectum

moderate

regional

moderate expression: see section 10 11

midgut

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3468
Entity Detected:	Rnf186, ring finger protein 186 ( MGI:1914075)
Sequence:	sense strand is shown >T3468 TGGCCTCGAGCAGATTCGGACGAGGCTATGTCCTCAGAGGCTAGTGGGTTCTGCCGCTTCAGCAACTCGG CCAGCCAACTGGACAGGAGAAGAAGAACAGGACACTGTAAGTGTCAACCGTGTAGCTGCCCGGCGCCTGG CTGTACACTTGCTCTTGTTGGCTCTTGTCATTGTCCTTATCCTGCCTTTCATCTACCCGGGTGTCATCCG GTGGGTGTTGGCCTTTGTCATTGCTCTGGCTCTCCTGATGTCCACCCTATTCTGCTGTCACCCCCAGAGC CAGAACAGCAACTGGCTTTGCCCCAGGACTCTGTTCTGCAGAGAGCAAAAGCAAACCCAGATCACTTCTA TTGCCTGAGAACTCTGGATCCCTACAGGCCCAGTGCTGCCAAGGTTCCCTCCTCCCCCAGACCTCACAGT CTGCATGGGTAAGGGGCTAGGGGCCGCCTTTCAAAATCACATTCGAACTGGACTTGCGTGTCAAAGCCAG ACTGGCCATGTTGGATCAAGGATTCTGTGCTGGAACAGTGGGATCGCACCTGCCTGTTCCTGTGT
Notes:	The probe template was PCR amplified from IMAGE:331375 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:331375 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20511 same embryo
	EMAGE:20514 same embryo
	EMAGE:20512 same embryo
	EMAGE:20510 same embryo
	EurExpress:euxassay_002022 same experiment
	MGI:4827771 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source