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EMAGE:20553

Lrp1b low density lipoprotein-related protein 1B (deleted in tumors) ( MGI:2151136)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20553 EMAGE:20553 EMAGE:20553 EMAGE:20553 EMAGE:20553
"Pseudo-wholemount" of euxassay_013815. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013815_01 euxassay_013815_02 euxassay_013815_03 euxassay_013815_04
EMAGE:20553 EMAGE:20553 EMAGE:20553 EMAGE:20553 EMAGE:20553
euxassay_013815_05 euxassay_013815_06 euxassay_013815_07 euxassay_013815_08 euxassay_013815_09
EMAGE:20553 EMAGE:20553 EMAGE:20553 EMAGE:20553 EMAGE:20553
euxassay_013815_10 euxassay_013815_11 euxassay_013815_12 euxassay_013815_13 euxassay_013815_14
EMAGE:20553 EMAGE:20553 EMAGE:20553 EMAGE:20553 EMAGE:20553
euxassay_013815_15 euxassay_013815_16 euxassay_013815_17 euxassay_013815_18 euxassay_013815_19
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euxassay_013815_20 euxassay_013815_21 euxassay_013815_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20553Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20553_wholemount_strong.wlz
20553_wholemount_moderate.wlz
20553_wholemount_weak.wlz
20553_wholemount_possible.wlz
20553_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20553_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
diencephalon roof plate
weak weak
regionalweak expression: see section 10
medulla oblongata floor plate
weak weak
regionalweak expression: see section 11
spinal cord floor plate
weak weak
regionalweak expression: see section 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38300
Entity Detected:Lrp1b, low density lipoprotein-related protein 1B (deleted in tumors) ( MGI:2151136)
Sequence:sense strand is shown

>T38300
ATATAGAGGAGGCAGCAATGGATGGCACTCTGAGGAGGGTTTTAGTACAAAAAAACTTACAGAGACCCAC
AGGTCTGACTGTTGATCATTTTGGTGAACGCATCTACTGGGCAGACTTTGAGCTCTCCATCATTGGCAGT
GTCCTGTATGATGGTTCCAGTCCAGTGGTCTCTGTCAGCAGCAAACAAGGTTTGTTGCATCCTCACAGAA
TTGATGTCTTTGAAGATTATATATATGGAGCAGGACCTAAAAATGGTATATTCAGAGTTCAAAAATTTGG
TCATGGCTCAGTAGAGGTCCTAGCTTTAGGCGTTGACAAAACAAAGAGCATTTTGGTATCACATCGCTAT
AAACAACTAAATTTACCCAATCCTTGCTTGGATTTGTCATGTGACTTTCTTTGCTTACTGAATCCTTCTG
GAGCCACATGTATCTGCCCAGAGGGAAAATATATGATGAATGGGACCTGCCATGATGACAGTCTGTTAGA
TGATTCTTGCAAACTGACTTGTGAAAATGGAGGAAGATGCATTTTAAATGAGAAGGGTGATCTGAGATGT
CACTGCTGGCCCAGTTATTCAGGAGGAAGGTGTGAAGTCAATCATTGTAGCAATTACTGTCAGAATGGAG
GGACCTGCATA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 145671. Forward Primer - name:145671_F_cDNA_Lrp1b, sequence:ATATAGAGGAGGCAGCAATGGA; Reverse Primer - name:145671_N_SP6_cDNA_Lrp1b, sequence:TATGCAGGTCCCTCCATTCTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20554 same embryo
 EMAGE:20555 same embryo
 EMAGE:20552 same embryo
 EurExpress:euxassay_013815 same experiment
 MGI:4825982 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS