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EMAGE:20602

Itpr2 inositol 1,4,5-triphosphate receptor 2 ( MGI:99418)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20602 EMAGE:20602 EMAGE:20602 EMAGE:20602 EMAGE:20602
"Pseudo-wholemount" of euxassay_013833. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013833_01 euxassay_013833_02 euxassay_013833_03 euxassay_013833_04
EMAGE:20602 EMAGE:20602 EMAGE:20602 EMAGE:20602 EMAGE:20602
euxassay_013833_05 euxassay_013833_06 euxassay_013833_07 euxassay_013833_08 euxassay_013833_09
EMAGE:20602 EMAGE:20602 EMAGE:20602 EMAGE:20602 EMAGE:20602
euxassay_013833_10 euxassay_013833_11 euxassay_013833_12 euxassay_013833_13 euxassay_013833_14
EMAGE:20602 EMAGE:20602 EMAGE:20602 EMAGE:20602 EMAGE:20602
euxassay_013833_15 euxassay_013833_16 euxassay_013833_17 euxassay_013833_18 euxassay_013833_19
EMAGE:20602 EMAGE:20602 EMAGE:20602
euxassay_013833_20 euxassay_013833_21 euxassay_013833_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20602Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20602_wholemount_strong.wlz
20602_wholemount_moderate.wlz
20602_wholemount_weak.wlz
20602_wholemount_possible.wlz
20602_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20602_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
medulla oblongata basal plate mantle layer
strong strong
single cellstrong expression: see section 12 moderate expression: see section 11 weak expression: see section 10 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38668
Entity Detected:Itpr2, inositol 1,4,5-triphosphate receptor 2 ( MGI:99418)
Sequence:sense strand is shown

>T38668
AGCTTCTCTGGACAGGATTCAGATAAGAAGGGCATTTCAATGTCCGATATCCAGTGTTTGCTGGACAAAG
AAGGAGCATCCGAACTTGTCATCGATGTCATTGTGAACACCAAAAACGACAGGATCTTTTCCGAAGGAAT
TTTGCTTGGCATTGCCTTGCTGGAAGGAGGAAACACACAGACCCAGTATTCTTTCTACCAGCAGTTGCAT
GAACAAAAAAAGTCAGAGAAATTTTTCAAAGTCCTTTATGATCGGATGAAGGCTGCTCAGAAAGAGATCA
GATCCACGGTGACCGTCAACACCATAGACTTAGGGAGCAAGAAGAGAGAAGAGGACAGTGACGTCATGGC
CCTGGGCCCTCGGATGAGAGTGAGAGACTCATC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 153502. Forward Primer - name:153502_F_cDNA_TC1483888, sequence:AGCTTCTCTGGACAGGATTCAG; Reverse Primer - name:153502_N_SP6_cDNA_TC1483888, sequence:GATGAGTCTCTCACTCTCATCCG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20604 same embryo
 EMAGE:20601 same embryo
 EMAGE:20603 same embryo
 EMAGE:20605 same embryo
 EMAGE:20600 same embryo
 EurExpress:euxassay_013833 same experiment
 MGI:4825666 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS