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EMAGE:20603

Sorcs3 sortilin-related VPS10 domain containing receptor 3 ( MGI:1913923)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20603 EMAGE:20603 EMAGE:20603 EMAGE:20603 EMAGE:20603
"Pseudo-wholemount" of euxassay_013842. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013842_01 euxassay_013842_02 euxassay_013842_03 euxassay_013842_04
EMAGE:20603 EMAGE:20603 EMAGE:20603 EMAGE:20603 EMAGE:20603
euxassay_013842_05 euxassay_013842_06 euxassay_013842_07 euxassay_013842_08 euxassay_013842_09
EMAGE:20603 EMAGE:20603 EMAGE:20603 EMAGE:20603 EMAGE:20603
euxassay_013842_10 euxassay_013842_11 euxassay_013842_12 euxassay_013842_13 euxassay_013842_14
EMAGE:20603 EMAGE:20603 EMAGE:20603 EMAGE:20603 EMAGE:20603
euxassay_013842_15 euxassay_013842_16 euxassay_013842_17 euxassay_013842_18 euxassay_013842_19
EMAGE:20603 EMAGE:20603 EMAGE:20603 EMAGE:20603 EMAGE:20603
euxassay_013842_20 euxassay_013842_21 euxassay_013842_22 euxassay_013842_23 euxassay_013842_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20603Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20603_wholemount_strong.wlz
20603_wholemount_moderate.wlz
20603_wholemount_weak.wlz
20603_wholemount_possible.wlz
20603_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20603_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
diencephalon lateral wall mantle layer
weak weak
regionalweak expression: see section 10 11 14 15 16 17
olfactory cortex marginal layer
moderate moderate
regionalmoderate expression: see section 11 12 16 17 weak expression: see section 10 15 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38802
Entity Detected:Sorcs3, sortilin-related VPS10 domain containing receptor 3 ( MGI:1913923)
Sequence:sense strand is shown

>T38802
CTCCCATGCTCCACACTACATACACATACAACACAGACCATTCCCCAGTTCAATTGCAGGACGTAGGTTG
AGGGGGAGTGGACGTAGCTGCCAAAGGCGCGCACACCTTTCCGAAAGAGGCCCTGCCTCATGCATGTCGA
AACTGAGGCTAAAGATGGTGTATTGTATATAACTACAATGTAAATAGCTTTTTATTTCCTAAGAAATAAT
TTAATGTTTAGTAAAAAAGAAAACAGAAAAAAAGAAAGATGTGTGTGTTGGCTTCTGCACTCACCTTCAG
AGCTGACCGCCCCCCAGCCTGCCTGATGTGTTGGATTCTGGATGCTCTCCAGCTGTCTTTCACACTCAAC
TCTTGCGTCTCTGTCCACACCATAGCCTGTTTCTGCTTATGTATATAAAGGGCATGGGGGAGGGCTTCTT
CAGGGCAATTGACAAAGGAAGGACTTCAGTGGCGTTCAGGAACATGGGGGTTGTTTTTCTTCCTAGGATA
ACATGGAAGGAGTCACAAAGACTAGTAGAGACTTCAAACTCTCATAAAGAAATAAAGAAAAAAAAGGTAT
TTATCAGCCTGAGAAAGCAGTCCTCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 236462. Forward Primer - name:165617_F_cDNA_Sorcs3, sequence:CTCCCATGCTCCACACTACATA; Reverse Primer - name:165617_N_SP6_cDNA_Sorcs3, sequence:AGAGGACTGCTTTCTCAGGCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20604 same embryo
 EMAGE:20602 same embryo
 EMAGE:20601 same embryo
 EMAGE:20605 same embryo
 EMAGE:20600 same embryo
 EurExpress:euxassay_013842 same experiment
 MGI:4828382 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS