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EMAGE:3224

Satb1 special AT-rich sequence binding protein 1 ( MGI:105084)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3224
0706d1.jpg This embryo was treated with ProteinaseK for either 3min or 30min.

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:3224Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3224_wholemount_strong_3D_1.wlz
3224_wholemount_moderate_3D_1.wlz
3224_wholemount_weak_3D_1.wlz
3224_wholemount_possible_3D_1.wlz
3224_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3224_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
central nervous system
possible possible
Authors stated that in some instances they could not determine expression levels with certainty.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3506384
Entity Detected:Satb1, special AT-rich sequence binding protein 1 ( MGI:105084)
Sequence:sense strand is shown

>MGI:3506384
ACACAGCTCTGCTGCCCAAGCCAAAGGGCTCATCCAGGTTGGGAAGTGGAATCCAGTTCCACTGTCGTAT
GTGACAGATGCCCCTGATGCCACGGTGGCAAACATGCTTCAAGATGTGTATCATGTGGTCACCCTCAAAA
TTCAGTTACACAGTTGCCCTAAACTAGAAGACTTGCCTCCTGAACAATGGTCGCACACCACAGTAAGGAA
TGCTCTGAAGGACTTACTGAAAGATATGAACCAGAGTTCGTTGGCCAAGGAGTGCCCCCTTTCACAGAGC
ATGATCTCCTCCATTGTGAACAGCACGTACTATGCAAATGTCTCAGCAGCAAAATGTCAAGAATTTGGAA
GGTGGTACAAACATTTCAAGAAGACAAAGGATATGATGGTTGAGATGGATAGTCTGTCTGAACTATCCCA
GCAAGGTGCCAACCACGTCAATTTTGGCCAGCAGCCTGTCCCAGGAAACACAGCTGAGCAGCCTCCATCC
CCTGCGCAGCTCTCTCACGGCAGTCAGCCCTCTGTCCGGACCCCTCTTCCGAACCTGCACCCTGGGCTTG
TGTCAACACCGATCAGTCCTCAGCTGGTCA
nt 561 - nt 1150 of U05252.1
Notes:The template DNA sequence was PCR amplified from E13.5 or P0 mouse brain, embryonic kidney or testis cDNA using: 5' primer: ACACAGCTCTGCTGCCCAAGCC 3'primer: GACCAGCTGAGGACTGATCGG
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Yu J., Tenzen T., Gray P.A., Fu H., Luo P., Zhao Q., Ferrari A., Yuk D.I., Tsung E.F., Cai Z., Alberta J.A., Cheng L.P., Liu Y., Stenman J.M., Valerius M.T., Billings N., Kim H.A., Greenberg M.E., Rowitch D.H., Stiles C.D., Ma Q., McMahon A.P. Indexed by GXD, Spatially mapped by EMAGE.
Principal investigator:Andrew P. McMahon, Molecular and Cellular Biology, Harvard, Department of Molecular and Cellular Biology Harvard University 16 Divinity Ave Room 1059, Cambridge, U.S.A MA 02138
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1126/science.1104935] [ PMID:15618518] Gray PA, 2004 Mouse brain organization revealed through direct genome-scale TF expression analysis. Science (306):2255-7
Acknowledgments:This work was supported by the Charles Dana Foundation, the National Institute of Neurological Disorders and Stroke (QM, CDS, DHR & APM), the National Institute of Dental and Craniofacial Research (QM), the National Institute of Diabetes and Digestive and Kidney Diseases (APM), a Ford Foundation Postdoctoral Fellowship for Minorities (PAG), a Parker B. Francis Fellowship in Pulmonary Medicine (PAG) and the Pew Trust (QM).
Links:MGI:3509079 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE