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EMAGE:3406

Six3 sine oculis-related homeobox 3 homolog (Drosophila) ( MGI:102764)
TS15
in situ hybridisation

Data Images
EMAGE:3406
Figure 4C (inset) of Bernier et al., 2001 [PMID:11641222] . Copyright: This image is from Bernier et al., Development 128:3987-3994 (2001), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:3406EMAGE:3406Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3406_voxel_strong_3D_1.wlz
3406_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3406_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
optic vesicle
detected detected
regionalExpression in the retinal plate.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2152921
Entity Detected:Six3, sine oculis-related homeobox 3 homolog (Drosophila) ( MGI:102764)
Notes:The Six3 probe used in this study by Bernier et al., 2001 [PMID:11641222] is described as a "1.4 kb Six3 cDNA (Oliver et al., 1995 [PMID:8575305] )". Editors Note: Oliver et al. do not describe any 1.4kb clones of Six3, yet they did use various "RNA probes generated from various Bluescript KS II+ subclones containing different coding regions of Six3" for in situ hybridisation. However, the sequence/regions covered of each of these clones are not discussed.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Bernier G; Vukovich W; Neidhardt L; Herrmann BG; Gruss P, 2001 [PMID:11641222] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11641222] Bernier G, Vukovich W, Neidhardt L, Herrmann BG, Gruss P 2001 Isolation and characterization of a downstream target of Pax6 in the mammalian retinal primordium. Development (128):3987-94
 [ PMID:8575305] Oliver G, Mailhos A, Wehr R, Copeland NG, Jenkins NA, Gruss P 1995 Six3, a murine homologue of the sine oculis gene, demarcates the most anterior border of the developing neural plate and is expressed during eye development. Development (121):4045-55
Links:MGI:2153753 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI