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EMAGE:3432

Nkx2-1 NK2 homeobox 1 ( MGI:108067)
TS15 (23 Somite no.)
immunohistochemistry

Data Images
EMAGE:3432 EMAGE:3432
Figure 1A of Corbin et al., 2003 [PMID:12930780] . Copyright: This image is from Corbin et al., Development 130, 4895-4906 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 1C of Corbin et al., 2003 [PMID:12930780] . Copyright: This image is from Corbin et al., Development 130, 4895-4906 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Note: Fig 1A shows PAX6 in green and NKX2.1(TITF1) in red. Arrow, boundary of PAX6 and NKX2.1(TITF1) expression in the ventral telencephalon. 1C also shows SHH expression innblue.
Expression Pattern Description
Spatial Annotation:
EMAGE:3432EMAGE:3432Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3432_voxel_strong_3D_1.wlz
3432_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3432_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
telencephalon
detected detected
regionalExpression of Pax6 and Nkx2.1(Titf1) forms a boundary in the ventral telencephalon.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:2676172
Entity Detected:Nkx2-1, NK2 homeobox 1 ( MGI:108067)
Notes:The anti-Titf1(Nkx2.1) antibody used in this study by Corbin et al., 2003 [PMID:12930780] was either a "mouse anti-Nkx2.1 (1:2000, DAKO, CA, USA)" or a "rabbit anti-Nkx2.1 (1:150, Biopat, Italy)". It is unspecified which of these were used in this actual experiment.
Antibody Type:monoclonal
Specimen
Organism:mouse
Age:23 Somite no.
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
General Information
Authors:Corbin JG; Rutlin M; Gaiano N; Fishell G, 2003 [PMID:12930780] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.00717] [ PMID:12930780] Corbin JG, Rutlin M, Gaiano N, Fishell G 2003 Combinatorial function of the homeodomain proteins Nkx2.1 and Gsh2 in ventral telencephalic patterning. Development (130):4895-906
Links:MGI:2676233 same experiment
 EMAGE:3377 same section & embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI