EMAGE mouse gene expression spatial database

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EMAGE:3507

En2 engrailed 2 ( MGI:95390)
TS16 (10.0-10.5 dpc)
in situ hybridisation

Data Images


Fig1B.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(01)00618-9] Mech Dev 111: 125-36, Degenhardt K; Rentschler S; Fishman G; Sassoon DA, Cellular and cis-regulation of En-2 expression in the mandibular arch. Copyright 2002.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Note: Presence of engrailed in the midbrain/hindbrain (blue arrow) region as well as the first arch (red arrow) is indicated.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

3507_wholemount_strong_3D_1.wlz

3507_wholemount_moderate_3D_1.wlz

3507_wholemount_weak_3D_1.wlz

3507_wholemount_possible_3D_1.wlz

3507_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3507_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

future midbrain

detected

regional

Expression at the midbrain-hindbrain junction.

future hindbrain

detected

regional

Expression at the midbrain-hindbrain junction.

1st branchial arch mandibular component

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:2450115
Entity Detected:	En2, engrailed 2 ( MGI:95390)
Sequence:	sense strand is shown >MGI:2450115 GATCTTTCTTTCATTTTTCTTTTCTCCCTTTTTTTAATCCCTTAAATTCCATTATGGGACATTGGACACT TCTTCCCCCGAAAGAAAAAAAATTTAAAACAACTTGCTGAAGTCCAAAGATTTTTATTGCTGCATTTCAC AGGACTGTGAACCGAATAAATAGCTCCTATTTGGTCTGTGGGCTCTGCCGCTTGCTTTGTGCTGGCTCGG ACAGCACTGTAGGAAGCGCCAGCCAGAGTGGTGATGGAGAGCTCTGGTGGGGAGAGAGGGCGCAGTTCTT TGCTGAGGTCTGAGAAAGTCCTTGCAGGCCAGCAGCTCTCCCGGAACCTCTCCGCCTGCTTCCTACGCAG CCTCTCTTCTCAACCATCTCAGAGGCCCGTGCTGGCAGCCACCGTGTGTCTGCGTCTGTGTCGATGACCC CTGTCCGCCACAGCCCCGTAGCGACCTGCCAGCGCCAACTCTCCCGCCACCGTGGCCAGCGTTGGGGCCT GGGGAGCAGTGGGAGTGTGTCCTGAAGGCTTGAAGGCTTTGCTGCCGGGACAGCTAGCCCTGACCCAAAA GCCCTCACCGAGATAGGCCTTGGGGCCTGGCAGAGCGAGTTGGAATCATCTTCCTTTCCTTTTTCTTCCC TCTTTTTTCTTTTTCATTTATTTTTGAATGGTAAATACATGTATGTGTACATACTTGTGTATGCATGTGC ACGCACACCCCAGGCCTGTGTGAAGCTGGGGTTCTGGAGCTCACAGCTACCTTGACTTTCAAAGGAACTC GAATCCTCCCGAAT
	nt 2106 - nt 2889 of NM_010134.2
Notes:	The En2 probe used in this study by Degenhardt et al., 2002 [PMID:11804784] was "a 0.8 kb BgIII-XbaI fragment from the 3' UTR of En-2". Editors Note: Digestion of the En2 mouse RefSeq mRNA sequence NM_010134.2 with BglII and XbaI identifies a 784nt 3'-UTR fragment (co-ordinates 2106-2889 as shown above).
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD-1
Age:	10.0-10.5 dpc
Theiler Stage:	TS16
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:	4% paraformaldehyde
Staining procedure:	alkaline phosphatase + BMpurple

General Information

Authors:	Degenhardt K; Rentschler S; Fishman G; Sassoon DA, 2002 [PMID:11804784] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/S0925-4773(01)00618-9] [ PMID:11804784] Degenhardt K, Rentschler S, Fishman G, Sassoon DA 2002 Cellular and cis-regulation of En-2 expression in the mandibular arch. Mech Dev (111):125-36
Links:	MGI:2450116 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source