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EMAGE:4010

Marcks myristoylated alanine rich protein kinase C substrate ( MGI:96907)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4010 EMAGE:4010
Fig3H. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2003.11.024] Dev Biol 268: 24-38, Powles N; Babbs C; Ficker M; Schimmang T; Maconochie M, Identification and analysis of genes from the mouse otic vesicle and their association with developmental subprocesses through in situ hybridization. Copyright 2004 Fig6. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2003.11.024] Dev Biol 268: 24-38, Powles N; Babbs C; Ficker M; Schimmang T; Maconochie M, Identification and analysis of genes from the mouse otic vesicle and their association with developmental subprocesses through in situ hybridization. Copyright 2004

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
In Fig3H, Cy indicates "cytoskeleton associated". Fig6 shows a cartoon summary of expression of genes with restricted patterns indentified in this screen: Marcks/Macs is listed as having expression in neuroblast/VIII ganglion.
Expression Pattern Description
Spatial Annotation:
EMAGE:4010Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4010_wholemount_strong_3D_1.wlz
4010_wholemount_moderate_3D_1.wlz
4010_wholemount_possible_3D_1.wlz
4010_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4010_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
otocyst
detected detected
regionalExpression in anterior and ventral otic vesicle.
endolymphatic appendage
detected detected
Expression in endolymphatic duct (on the dorsal side of the otic vesicle).
acoustic viii ganglion
detected detected
Expression in the anterior/ventral otocyst in the neuroblast/VIII ganglion region (See Fig6).
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3510004
Entity Detected:Marcks, myristoylated alanine rich protein kinase C substrate ( MGI:96907)
Notes:The Marcks (Macs) probe used in this study by Powles et al., 2004 [PMID:15031102] was transcribed from clone np46 which was identified in a subtractive hybridisation screen. The exact probe sequence is not reported by the authors.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6 x CBA
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Powles N; Babbs C; Ficker M; Schimmang T; Maconochie M, 2004 [PMID:15031102] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1016/j.ydbio.2003.11.024] [ PMID:15031102] Powles N, Babbs C, Ficker M, Schimmang T, Maconochie M 2004 Identification and analysis of genes from the mouse otic vesicle and their association with developmental subprocesses through in situ hybridization. Dev Biol (268):24-38
Links:MGI:3510340 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI