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EMAGE:4040

S100a10 S100 calcium binding protein A10 (calpactin) ( MGI:1339468)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4040
Figure 2M. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2005.04.009] Gene Expr Patterns 6: 94-101, Machka C; Kersten M; Zobawa M; Harder A; Horsch M; Halder T; Lottspeich F; Hrabe de Angelis M; Beckers J, Identification of Dll1 (Delta1) target genes during mouse embryogenesis using differential expression profiling. Copyright 2005.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: Lateral view. Arrows indicate segmented expression and cranial ganglia are indicated V, VII/VIII, IX, and X.
Expression Pattern Description
Spatial Annotation:
EMAGE:4040Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4040_wholemount_strong_3D_1.wlz
4040_wholemount_moderate_3D_1.wlz
4040_wholemount_weak_3D_1.wlz
4040_wholemount_possible_3D_1.wlz
4040_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4040_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
head mesenchyme derived from neural crest
detected detected
trunk mesenchyme derived from neural crest
detected detected
facial vii ganglion
detected detected
glossopharyngeal ix ganglion
detected detected
trigeminal v ganglion
detected detected
vagus x ganglion
detected detected
tail mesenchyme derived from neural crest
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3617181
Entity Detected:S100a10, S100 calcium binding protein A10 (calpactin) ( MGI:1339468)
Sequence:sense strand is shown

>MGI:3617181
TCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGGGGCCCAGGTTTCGACAGACTCTTCAAAAT
GCCATCCCAAATGGAGCACGCCATGGAAACCATGATGCTTACGTTTCACAGGTTTGCAGGCGACAAAGAC
CACTTGACAAAGGAGGACCTGAGAGTGCTCATGGAACGGGAGTTCCCTGGGTTTTTGGAAAATCAAAAGG
ATCCTCTGGCTGTG
nt 1 - nt 224 of NM_009112.1
Notes:The S100a10 probe template used in this study by Horsch et al., 2005 [PMID:15979417] is 224bp long and was amplified from E10.5 embryonic total RNA by reverse transcription-PCR and cloned using the TopoTA Cloning Kit (Invitrogen). Primersused: 5' TCGTGGTGTGCCCAGCTCTT 3' (forward) and 5' CACAGCCAGAGGATCCTTTTGA 3' (reverse).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Machka C; Kersten M; Zobawa M; Harder A; Horsch M; Halder T; Lottspeich F; Hrabe de Angelis M; Beckers J, 2005 [PMID:15979417] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.modgep.2005.04.009] [ PMID:15979417] Machka C, Kersten M, Zobawa M, Harder A, Horsch M, Halder T, Lottspeich F, Hrabe de Angelis M, Beckers J 2005 Identification of Dll1 (Delta1) target genes during mouse embryogenesis using differential expression profiling. Gene Expr Patterns (6):94-101
Links:MGI:3617195 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI