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EMAGE:4099

Chrd chordin ( MGI:1313268)
TS14 (9.0 dpc)
in situ hybridisation

Data Images
EMAGE:4099
Fig2B. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2001.0479] Dev Biol 240: 457-73, Stottmann RW; Anderson RM; Klingensmith J, The BMP antagonists Chordin and Noggin have essential but redundant roles in mouse mandibular outgrowth. Copyright 2001

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: ov - optic vesicle; p - pharynx; BA1 - first branchial arch.
Expression Pattern Description
Spatial Annotation:
EMAGE:4099Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4099_wholemount_strong_3D_1.wlz
4099_wholemount_moderate_3D_1.wlz
4099_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4099_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
detected detected
regionalExpression is in the pharyngeal endoderm and ectoderm.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2156796
Entity Detected:Chrd, chordin ( MGI:1313268)
Sequence:sense strand is shown

>MGI:2156796
GCAGGCTGCTCCTTCGGCGGGAAGGTCTATGCCTTGGACGAGACGTGGCACCCGGACCTGGGGGAGCCTT
TTGGGGTGATGCGCTGCGTGCTGTGCGCCTGTGAAGCGCCTCAGTGGGCTCGCCGTGGGAGGGGTCCTGG
CAGGGTCAGCTGCAAGAACATCAAACCTCAGTGCCCCACCCTGGCCTGCAGGCAGCCGCGCCAGCTGCCA
GGACACTGCTGCCAGACCTGCCCGCAGGAGCGCAGCAATCTAGACCCACAGCCCGCTGGCCTGGTCTTCG
AGTATCCAAGGGATCCAGAGCATCGCAGTTACAGTGATCGAGGGGAACCCGGCGTTGGGGAGCGGACCCG
TGCTGATGGCCACACAGACTTTGTGGCGCTGCTGACAGGACCGAGGTCGCAGGCGGTAGCTCGTGCTCGA
GTCTCTTTGCTGCGCTCAAGTTTACGCTTCTCTGTCTCCTACCAGCGGCTGGACCGTCCCAGCAGGGTTC
GTTTCACAGATCCCACAGGCAACATCCTGTTTGAACACCCTGCAACCCCCACCCAGGATGGCCTGGTCTG
TGGGGTGTGGCGGGCAGTGCCTCGGCTGTCTGTGAGGCTCCTGAGGGCAGAGCAGCTTCGGGTAGCCCTT
GTGACATCCACTCACCCTTCAGGAGAAGTCTGGGGGCCTCTCATTTGGCAGGGTGCTCTCGCTGCAGAGA
CCTTCAGTGCCATCCTGACCCTGGAAGACCCACTGCAGCGGGGTGTGGGGGGCATAGCCCTGCTCACCCT
CAGTGACACAGAAGATTCCTTGCATTTTTTGCTGCTCTTCCGGGGTCTGCTGGGAGGACTAGCTCAGGCT
CCCTTGAAGCTTCAGATTCTCCACCAGGGACAGCTACTTCGGGAGCTCCAGGCCAACACCTCCGCTCAGG
AGCCAGGTTTTGCTGAGGTGCTGCCCAGCCTTACAGACCAAGAGATGGACTGGTTGGAGCTGGGGGAGCT
GCAGATGGTCCTAGAGAAGGCGGGTGGACCAGAGCTACGCATCAGTGGATACATCACCACCAGGCAGAGC
TGTGATGTCCTTCAAAGTGTCCTTTGTGGGGCGGATGCCCTGATCCCAGTCCAGACGGGTGCTGCTGGGT
CAGCCAGCTTCATATTGCTAGGAAATGGCTCCCTTATCTATCAGGTGCAAGTGGTAGGTACAGGTAGCGA
GGTGGTGGCCATGACACTGGAGACCAAGCCTCAGCGGAAGAACCAGCGCACTGTCCTGTGCCACATGGCT
GGACTCCAGCCGGGAGGACACATGGCTGTGGGTATGTGCTCTGGGCTGGGTGCCCGAGGGGCTCATATGC
TGCTACAGAACGAGCTGTTCCTGAATGTTGGTACCAAGGACTTCCCAGATGGAGAGCTTCGGGGGCATGT
GACTGCCCTATGCTACAGTGGGCACAGTGCCCGCTATGACAGATTGCCTGTGCCTCTGGCAGGGGCACTA
GTGCTGCCCCCTGTGCGGAGTCAGGCAGCAGGGCACGCCTGGCTCTCCTTGGATACACATTGTCACTTAC
ACTATGAAGTTCTATTGGCTGGGCTTGGTGGCTCAGAGCAAGGAACTGTCACTGCCCACCTCCTTGGGCC
TCCTGGGATGCCAGGGCCCCAGCGGCTGCTGAAGGGATTCTATGGCTCAGAGGCTCAAGGCGTGGTAAAA
GATCTGGAGCCTGTGCTGCTGCGGCACCTGGCACAGGGAACTGCCTCCCTGCTCATCACCACCAAGAGTA
GCCCCAGAGGAGAACTACGTGGGCAGGTGCACATTGCCAGTCAGTGTGAGGCGGGAGGCCTGCGTCTGGC
CTCAGAAGGAGTGCAGATGCCCTTGGCTCCAAATGGAGAGGCGGCTACGTCA
nt 145 - nt 2016 of AF096276.1
Notes:The Chrd probe used in this study by Stottmann et al., 2001 [PMID:11784076] is indicated as that previously used by Klingensmith et al., 1999 [PMID:10642791] , who describe it as the same as used by "Bachiller et al., submitted for publication". Editors note: Bachiller et al, 2000 [PMID:10688202] define the probe as "a subclone spanning nucleotides 145-2016 of the full-length mouse chordin complementary DNA (accession number AF096276), linearized with NotI and transcribed with T3 RNA polymerase".
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:ICR
Age:9.0 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Stottmann RW; Anderson RM; Klingensmith J, 2001 [PMID:11784076] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2001.0479] [ PMID:11784076] Stottmann RW, Anderson RM, Klingensmith J 2001 The BMP antagonists Chordin and Noggin have essential but redundant roles in mouse mandibular outgrowth. Dev Biol (240):457-73
 [ doi:10.1006/dbio.1999.9525] [ PMID:10642791] Klingensmith J, Ang SL, Bachiller D, Rossant J 1999 Neural induction and patterning in the mouse in the absence of the node and its derivatives. Dev Biol (216):535-49
 [ doi:10.1038/35001072] [ PMID:10688202] Bachiller D, Klingensmith J, Kemp C, Belo JA, Anderson RM, May SR, McMahon JA, McMahon AP, Harland RM, Rossant J, De Robertis EM 2000 The organizer factors Chordin and Noggin are required for mouse forebrain development. Nature (403):658-61
Links:MGI:2157407 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI