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EMAGE:4380

Bcor BCL6 interacting corepressor ( MGI:1918708)
TS16 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4380 EMAGE:4380
Fig3D. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2007.01.006] Gene Expr Patterns 7: 550-7, Wamstad JA; Bardwell VJ, Characterization of Bcor expression in mouse development. Copyright 2007 Fig3F. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2007.01.006] Gene Expr Patterns 7: 550-7, Wamstad JA; Bardwell VJ, Characterization of Bcor expression in mouse development. Copyright 2007

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: TB - tail bud. D is a sense control.
Expression Pattern Description
Spatial Annotation:
EMAGE:4380Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4380_wholemount_strong_3D_1.wlz
4380_wholemount_possible_3D_1.wlz
4380_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4380_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
strong strong
limb
strong strong
embryo
detected detected
There is less robust expression throughout the rest of the embryo than in limb, branchical arch or tail bud.
tail
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3714011
Entity Detected:Bcor, BCL6 interacting corepressor ( MGI:1918708)
Sequence:sense strand is shown

>MGI:3714011
ATCGATGGCCTGGCAGCACTGAGCATGGACCGCACAGGCCTCATCCGGGAAGGGCTCCGGGTCCCTGGAA
ACATCGTTTATTCTGGCTTGTGTGGATTGGGCTCAGAAAAAGGTCGGGAGGCTACCCCAAGCTCTCTCAG
TGGCCTTGGGTTTTCTTCGGAAAGAAATCCAGAGATGCAATTCAAACCGAATACTCCCGAGACAGTGGAG
GCTTCTGCTGTCTCTGGAAAACCCCCAAATGGCTTCAGTGCTATATACAAAACACCGCCTGGGATACAAA
AGAGCGCTGTAGTTACAGCGGAAAGCTTGAGTTTGGATAGGCCTGCCAGTGACAAACAGAGCCCTCTCAA
CATCAATGGTGCTAGTTACCTGCGGCTGCCCTGGGTCAACCCTTACATGGAGGGGGCCACTCCAGCCATA
TACCCTTTCCTCGACTCGCCAAATAAGTATTCACTGAATATGTACAAGGCCTTGCTACCTCAGCAGTCCT
ACGGCTTGGCCCAGCCACTGTATTCTCCTGTCTGCACCAGTGGGGAACGCTTCCTCTACCTGCCACCCCC
ACACTATGTCAACCCTCACATCCCCTCCTCCTTGGCTTCACCCATGAGGCTCTCCACACCTTCAGCCTCT
GCGGCCATTCCACCTCTCGTCCACTGCTCAGACAAAAGCCTCCCATGGAAGATGGGCGTCAACCCTGGGA
ACCCAGTAGATTCCCACAGTTACCCCCACATCCAAAACAGTAAACAGCCCCGGGTGACCTCTGCCAAGGC
AGTCAATAGTGGCCTGCCAGGGGACACAGCTCTCCTGTTGCCTCCTTCACCTCGGCCCTCAGCCCGGGTC
CACCTTCCAACTCAGCCCGCTGCAGAGACATACTCCGAGTTCCACAAGCACTATCCCAGGATCTCCACCT
CTCCCTCAGTCACCCTGACGAAGCCATACATGACAGCCAATAGCGAGTTCTCTACATCCAGGCTGTCCAA
T
nt 280 - nt 1260 of NM_029510.2
Notes:The Bcor probe template used in this study by Wamstad & Bardwell, 2007 [PMID:17344103] is described as follows: An amplicon containing nucleotides 280-1294 of the Bcor open reading frame was generated by PCR from a bacterial artificial chromosome containing the mouse Bcor gene using the following primers, forward: 5'-TACGGGTACCATCGATGGCCTGGCAGCACT-3' and reverse: 5'-CATGTCTAGATTGGACAGCCTGGATGTAGAG-3'. The PCR amplicon was gel purified and directly cloned into the Kpn and Xba restriction sites of a pBluescript KS+ vector. Editors note: although the authors state that the probe contains nucleotides 280-1294 of the Bcor ORF, when these primer sequences are BLASTed against the 3 Bcor mRNA RefSeq sequences: NM_029510.2 (transcript variant a); NM_175044.2 (transcript variant b) and NM_175045.2 (transcript variant c), the resulting start and end points for the PCR amplified fragment are nt280-1260, nt280-1260 and nt466-1446 respectively, the intervening sequence of which is identical for all three.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:9.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Wamstad JA; Bardwell VJ, 2007 [PMID:17344103] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.modgep.2007.01.006] [ PMID:17344103] Wamstad JA, Bardwell VJ 2007 Characterization of Bcor expression in mouse development. Gene Expr Patterns (7):550-7
Links:MGI:3714289 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI