Type: | in situ hybridisation probe |
Identifier: | MGI:9001 |
Entity Detected: | Bmp6, bone morphogenetic protein 6 ( MGI:88182) |
Sequence: | sense strand is shown
>MGI:9001
GACGGCCCGCGAGGAGCCCCCTCCAGGGCGGCTGAAGTCCGCTCCACTCTTCATGCTGGATCTCTACAAC
GCCCTGTCCAATGACGACGAAGAGGATGGGGCATCGGAGGGTGTGGGGCAAGAGCCTGGGTCCCACGGAG
GGGCCAGCTCGTCCCAGCTCAGGCAGCCGTCTCCCGGCGCTGCACACTCCTTGAACCGCAAGAGTCTCCT
GGCCCCGGGACCCGGTGGCGGTGCGTCCCCACTGACTAGCGCGCAGGACAGCGCTTTCCTCAACGACGCG
GACATGGTCATGAGCTTTGTGAACCTGGTGGAGTACGACAAGGAGTTCTCCCCACATCAACGACACCACA
AAGAGTTCAAGTTCAACCTATCCCAGATTCCTGAGGGTGAGGCGGTGACGGCTGCTGAGTTCCGCGTCTA
CAAGGACTGTGTGGTGGGGAGTTTTAAAAACCAAACCTTTCTTATCAGCATTTACCAAGTCTTGCAGGAG
CATCAGCACAGAGACTCTGACCTATTTTTGTTGGACACCCGGGTGGTGTGGGCCTCAGAAGAAGGTTGGC
TGGAATTTGACATCACAGCAACTAGCAATCTGTGGGTGGTGACACCGCAGCACAACATGGGGCTCCAGCT
GAGTGTGGTGACTCGGGATGGACTCCACGTCAACCCCCGTGCGGCGGGCCTGGTGGGCAGAGACGGCCCT
TACGACAAGCAGCCCTTCATGGTGGCCTTCTTCAAGGTGAGCGAGGTCCACGTGCGCACCACCAGGTCAG
CCTCCAGTCGGCGGCGGCAGCAGAGTCGCAACCGGTCCACCCAGTCGCAGGACGTGTCCCGGGGCTCCGG
TTCTTCAGACTACAACGGCAGTGAGTTAAAAACAGCTTGCAAGAAGCATGAGCT
|
| nt 564 - nt 1457 of NM_007556.1 |
Notes: | The Bmp6 (Vgr1) probe used in this study by Furuta et al., 1997 [PMID:12930780] was "a 900 bp fragment of Bmp6 (Jones et al., 1991 [PMID:1893873] )". Jones et al. in turn refer to Lyons et al., 1989 [PMID:2481605] who describe "an 893-bp SacI-EcoRI fragment from the 5' region of the Vgr-1 cDNA (Lyons et al., 1989 [PMID:2734307] )." As restriction analysis of Bmp6/Vgr1 clones in the public databases (eg NM_007556.1) finds an internal SacI cut site, but no 5' EcoRI site, this 5' EcoRI site is presumably linker derived. The fragment shown above extends 5' from the SacI site at nt1457 for 893nt. |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |