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EMAGE:6486

Fbxo4 F-box protein 4 ( MGI:2146220)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6486 EMAGE:6486 EMAGE:6486 EMAGE:6486 EMAGE:6486
"Pseudo-wholemount" of euxassay_016326. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016326_01 euxassay_016326_02 euxassay_016326_03 euxassay_016326_04
EMAGE:6486 EMAGE:6486 EMAGE:6486 EMAGE:6486 EMAGE:6486
euxassay_016326_05 euxassay_016326_06 euxassay_016326_07 euxassay_016326_08 euxassay_016326_09
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euxassay_016326_10 euxassay_016326_11 euxassay_016326_12 euxassay_016326_13 euxassay_016326_14
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euxassay_016326_15 euxassay_016326_16 euxassay_016326_17 euxassay_016326_18 euxassay_016326_19
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euxassay_016326_20 euxassay_016326_21 euxassay_016326_22 euxassay_016326_23 euxassay_016326_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6486Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6486_wholemount_strong.wlz
6486_wholemount_moderate.wlz
6486_wholemount_weak.wlz
6486_wholemount_possible.wlz
6486_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6486_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
esophagus
weak weak
regionalweak expression: see section 11 12
mandible
weak weak
regionalweak expression: see section 05 06 07 08 09 10 11 14 15 16 17 18 19 20
meckel's cartilage
weak weak
regionalweak expression: see section 05 06 07 08 09 10 13 14 17 18 19
maxilla
weak weak
regionalweak expression: see section 05 08 09 17 18 19 20
urethra of male
weak weak
regionalweak expression: see section 13 14 15
lung
moderate moderate
regionalmoderate expression: see section 06 07 09 10 11 12 13 14 15 16 weak expression: see section 03 04 05 08 17 18 19 20
orbito-sphenoid
weak weak
regionalweak expression: see section 01 02 06 07 08 18 19 20
viscerocranium
weak weak
regionalExpression in the turbinate bone.
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2983
Entity Detected:Fbxo4, F-box protein 4 ( MGI:2146220)
Sequence:sense strand is shown

>T2983
TCGANGCCAGAATTCGGACGAGGCTTCTGAGGACCTTTGCCCAACTGCTGGTTTACCTCACAGACAGATT
GATGGTATTGGATCTGGAGTCAACTTCCAGTTGAATAACCAGCAAAAATTCAACATCCTGATATTATACT
CGACTACCAGAAAAGAAAGAGACAGAGCAAGGGAGGAGCACACCAGCACCGTTAACAAGATGTTCAGCCT
ACAGAGTGAGGGGGACGAGCAGCAGGGCAGCCGCTACAGTGTGATCCCGCAGATTCAGAAAGTGTGTGAA
GTCGTAGACGGGTTCATCTACGTGGCAAACGCTGAAGCTCACCGACGTCATGAATGGCAAGATGAATTTT
CTCGGATTATGGCCATGACAGACCCAGCTTTTGGATCTTCAGGAAGACCCATGCTGGTTTTATCTTGTAT
TTCTCAAGCAGATGTAAAGAGAATGCCTTGTTTTTATTTAGCTCATGAGCTGCACCTCAGTCTTCTAAAC
CACCCATGGATGGTCCAGGATACAGAGGCTGAAACTCTGACTGGTTTTTTGAATGGCATTGAGTGGAT
Notes:The probe template was PCR amplified from IMAGE:1532996 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1532996 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6485 same embryo
 EMAGE:6488 same embryo
 EMAGE:6482 same embryo
 EMAGE:6484 same embryo
 EMAGE:6487 same embryo
 EMAGE:6483 same embryo
 EurExpress:euxassay_016326 same experiment
 MGI:4824808 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS