EMAGE mouse gene expression spatial database

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EMAGE:6626

Stam2 signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 ( MGI:1929100)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016355. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016355_01	euxassay_016355_02	euxassay_016355_03	euxassay_016355_04

euxassay_016355_05	euxassay_016355_06	euxassay_016355_07	euxassay_016355_08	euxassay_016355_09

euxassay_016355_10	euxassay_016355_11	euxassay_016355_12	euxassay_016355_13	euxassay_016355_14

euxassay_016355_15	euxassay_016355_16	euxassay_016355_17	euxassay_016355_18	euxassay_016355_19

euxassay_016355_20	euxassay_016355_21	euxassay_016355_22	euxassay_016355_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6626_wholemount_strong.wlz

6626_wholemount_moderate.wlz

6626_wholemount_weak.wlz

6626_wholemount_possible.wlz

6626_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6626_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

weak

regional

weak expression: see section 02 03 17

cornea

weak

regional

weak expression: see section 02

lower jaw incisor

moderate

regional

moderate expression: see section 10 weak expression: see section 13

lower jaw molar

weak

regional

weak expression: see section 07

upper jaw incisor

weak

regional

weak expression: see section 13

upper jaw molar

weak

regional

weak expression: see section 06

urethra of male

weak

regional

weak expression: see section 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2036
Entity Detected:	Stam2, signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 ( MGI:1929100)
Sequence:	sense strand is shown >T2036 GGCCTCGAGCCAGATTCGGCACGAGGGTCAGTGTAAATACTGCGTTCTCATTAAGTGGGGACCAGCCATT GAAGCTTAGCCGTTTTGGAATACTAAAATATGCCACATGACCTGTATATAAATAGTGTATATTAGTGTCT GTGAAGTTTAATATATGTAGAAATTTCTTCCTCCTACATTTTATTTTTTTCCATTCATAAATTTGTGTTG CAGAGTTATTGTTAGCCTAAAATATTAAATCTGTATTGATCTGATTTCAGTATTAATACGATATGTAAAG ATATATATTTTCCCATGATCCAGTTGAACTGTACTTATTTTAAGATGTTTGTAGCTATTTATTTTCAGCC AAAGGAATAGTTTACCTTCAGATTTCTACATTGGGTTCATATTTAATGTTTTCTAACAAAAATTTTTGAA TTTCATTTTTCATATAAATCTTGATATATTAGAAAATTCTTAAAAATATATAGAATTTTATAGTATAAAA CATTTCCTTGGGTGTTAAGTGGAGTATGTTTGAAAAGTCAACAGTCAGCCAATGTTGTCTTTTTATTTGG TAATTTGCTTACAAAATTACCTACACA
Notes:	The probe template was PCR amplified from IMAGE:751808 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:751808 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6625 same embryo
	EMAGE:6627 same embryo
	EMAGE:6624 same embryo
	EurExpress:euxassay_016355 same experiment
	MGI:4828478 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source