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EMAGE:6661

Pex12 peroxisomal biogenesis factor 12 ( MGI:2144177)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6661 EMAGE:6661 EMAGE:6661 EMAGE:6661 EMAGE:6661
"Pseudo-wholemount" of euxassay_007264. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007264_01 euxassay_007264_02 euxassay_007264_03 euxassay_007264_04
EMAGE:6661 EMAGE:6661 EMAGE:6661 EMAGE:6661 EMAGE:6661
euxassay_007264_05 euxassay_007264_06 euxassay_007264_07 euxassay_007264_08 euxassay_007264_09
EMAGE:6661 EMAGE:6661 EMAGE:6661 EMAGE:6661 EMAGE:6661
euxassay_007264_10 euxassay_007264_11 euxassay_007264_12 euxassay_007264_13 euxassay_007264_14
EMAGE:6661 EMAGE:6661 EMAGE:6661 EMAGE:6661 EMAGE:6661
euxassay_007264_15 euxassay_007264_16 euxassay_007264_17 euxassay_007264_18 euxassay_007264_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6661Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6661_wholemount_strong.wlz
6661_wholemount_moderate.wlz
6661_wholemount_weak.wlz
6661_wholemount_possible.wlz
6661_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6661_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
esophagus
moderate moderate
regionalmoderate expression: see section 11
mandible
weak weak
spottedweak expression: see section 04 05 06 07 08 09 14 15 16
maxilla
weak weak
spottedweak expression: see section 05 06 07 15 16
lung
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1979
Entity Detected:Pex12, peroxisomal biogenesis factor 12 ( MGI:2144177)
Sequence:sense strand is shown

>T1979
GGCCTCGAGCCAGATTCGGCACGAGGGTGTGTTTAATTATGTGAGGAGTCACCAAGCCTGTCCCATTACA
GGGTATCCAACAGAAGTCCAGCATCTAATTAAACTCTACTCTCCAGAGAACTGAAAGAGCTTAGTGCATT
ATCTCACCACTCAAGTTCTATACTCTAAGGCCATGAGACGGTTTTAGTATGGCAGACAACAGTGATACTT
CCCTGATTCATAACTACATTAACTTTAAGTAGTTACATGGGTTTCTTTAAAAGGATATATCCATTCGATC
CTAACCTTTTGGTTTGCTCTCTACCAAGCTGGTTAAGCAGGAGAATCAGGATTATCGGTGGTGTGAGAGC
CAATGTGGAGGTGAGTTATAAAAGACCACCTCACTTAAAGAGTAAGGTTTTAAGTTGGATGTGGTAGTAT
AGTATCAACACTCTGGAGATGGAGGCAGGATTTTGAGTTCAAGGCCACCATAGGGAGACTGTCAAAAGTA
AAATGAAAGTGAGAGACTGATTAAGAGGGATAAAATATCACAGGAAACCTCAGGTTAGACCTTATAAAGC
AAGTGGT
Notes:The probe template was PCR amplified from IMAGE:644210 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:644210 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6662 same embryo
 EMAGE:6660 same embryo
 EMAGE:6663 same embryo
 EMAGE:6664 same embryo
 EurExpress:euxassay_007264 same experiment
 MGI:4827159 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS