EMAGE mouse gene expression spatial database

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EMAGE:6683

Lxn latexin ( MGI:107633)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003277. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003277_01	euxassay_003277_02	euxassay_003277_03	euxassay_003277_04

euxassay_003277_05	euxassay_003277_06	euxassay_003277_07	euxassay_003277_08	euxassay_003277_09

euxassay_003277_10	euxassay_003277_11	euxassay_003277_12	euxassay_003277_13	euxassay_003277_14

euxassay_003277_15	euxassay_003277_16	euxassay_003277_17	euxassay_003277_18	euxassay_003277_19

euxassay_003277_20	euxassay_003277_21	euxassay_003277_22	euxassay_003277_23	euxassay_003277_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6683_wholemount_strong.wlz

6683_wholemount_moderate.wlz

6683_wholemount_weak.wlz

6683_wholemount_possible.wlz

6683_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6683_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

strong

regional

strong expression: see section 13 14 15 moderate expression: see section 16

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 13 14 15 moderate expression: see section 16

telencephalon ventricular layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 19 20 21 22 23 24

medulla oblongata basal plate

moderate

regional

moderate expression: see section 10 11 17 18

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 11 12 13 14 15 16 17

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 moderate expression: see section 05 19 20 21 22 23 24

pons ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 moderate expression: see section 18 19 20

midbrain ventricular layer

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18

facial vii ganglion

strong

regional

strong expression: see section 05

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 21 22 moderate expression: see section 08 09 10 18 19 20 23

ventral grey horn

strong

regional

strong expression: see section 13 15 16 moderate expression: see section 12

spinal cord ventricular layer

strong

regional

strong expression: see section 13 14 15 16

dorsal root ganglion

strong

regional

strong expression: see section 10 17 18 moderate expression: see section 11 12 13 19 20 weak expression: see section 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3313
Entity Detected:	Lxn, latexin ( MGI:107633)
Sequence:	sense strand is shown >T3313 GATTATCCAAAACAAGTTACAGTGAACTGCACAGCTGAAGTTCTTTACCCCCAAATGGGACAAGGCTCTG CACCAGAAGTCAACTTCACATTCGAAGGAGAAATCGGCAAGAACCCAGATGAGGAAGATAACACATTTTA TCAAAGCCTCATGTCTCTGAAGAGACCGCTGGAAGCACAGGACATCCCAGACAATTTTGGAAATGTGTCT CCACAAATGAAGCCAGTCCAGCACTTAGCCTGGGTCGCCTGCGGTTATGTAATGTGGCAGAATTCGACTG AAGACACATGGTATAAAATGTTAAAAATTCAGACGGTCAAGCAAGTGCAAAGAAACGATGACTTCATTGA GTTAGATTACACCATTCTACTCCACGACATTGCATCGCAGGAGATTATTCCCTGGCAAATGCAAGTCCTG TGGCATCCACAGTACGGCACAAAAGTAAAACACAACAGCCGCCTCCCAAAGGAAGGGCAGGCGGAGTGAA CAAGACGCTAACCCGTAGACAACCGTCA
Notes:	The probe template was PCR amplified from IMAGE:2780939 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2780939 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6684 same embryo
	EMAGE:6686 same embryo
	EMAGE:6688 same embryo
	EMAGE:6687 same embryo
	EMAGE:6685 same embryo
	EurExpress:euxassay_003277 same experiment
	MGI:4826026 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source