EMAGE mouse gene expression spatial database

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EMAGE:6737

Gpr153 G protein-coupled receptor 153 ( MGI:1916157)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007373. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007373_01	euxassay_007373_02	euxassay_007373_03	euxassay_007373_04

euxassay_007373_05	euxassay_007373_06	euxassay_007373_07	euxassay_007373_08	euxassay_007373_09

euxassay_007373_10	euxassay_007373_11	euxassay_007373_12	euxassay_007373_13	euxassay_007373_14

euxassay_007373_15	euxassay_007373_16	euxassay_007373_17	euxassay_007373_18	euxassay_007373_19

euxassay_007373_20	euxassay_007373_21	euxassay_007373_22	euxassay_007373_23	euxassay_007373_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6737_wholemount_strong.wlz

6737_wholemount_moderate.wlz

6737_wholemount_weak.wlz

6737_wholemount_possible.wlz

6737_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6737_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 03 04 05 06 07 08 09 20 21 22 23 24

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

submandibular gland primordium mesenchyme

strong

regional

strong expression: see section 06 07 08 09 10 11 18 19 20 21 22

neural retina

strong

regional

strong expression: see section 04 moderate expression: see section 02 03 24

heart valve

strong

regional

strong expression: see section 12 moderate expression: see section 13 15

esophagus

strong

regional

strong expression: see section 13 14 15

mandible

strong

regional

strong expression: see section 05 06 07 08 09 10 11 19 20 21 22 23 24 moderate expression: see section 12 13 14 16 17 18

maxilla

strong

regional

strong expression: see section 06 07 08 09 10 11 19 20 21 22 23 moderate expression: see section 12 13 16 17 18

bladder

strong

regional

strong expression: see section 14 15 16 17

temporal bone petrous part

moderate

regional

moderate expression: see section 02

vault of skull

moderate

regional

moderate expression: see section 01 02 03

orbito-sphenoid

strong

regional

strong expression: see section 03 04 05 06 22 23 24 moderate expression: see section 01 02

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3333
Entity Detected:	Gpr153, G protein-coupled receptor 153 ( MGI:1916157)
Sequence:	sense strand is shown >T3333 TGGCCTCGAGCCAGATTCGGACGAGGCAAAATTCCACCATATTGCCTGTCTCCCACTATGTCGGCCGCTT CCGTGGTCCTGCTGTGAAGGCTGCTGGTGCCTATCGCAGAGAACCAAAGCTCGTGGAAGTGGACTTTCGT GTCTCAGTGCCCATCTCCAGTTTTGCCTCCCACAGGCAAAGTCCTTGGAAAGGGTGTCCTGTACCCCACT TCCCCTGCAAGAAGGGCTTTGCTCTCTCCTGGGTCTGGGCTGTGCCAGGGACATGGGTATTCCCAGGAGA GTCTGGGAGCTGGGAGCAGAGGGGGAGAGCTGTCACCAGACAGCGGACCCTCTGTGTTGGGACCTGGAGA TAGTCTTAAGGCCAGCACAGAACCAGCCTGTGTCTCCTTGTTCTTGAGACGTGGTGGACAGGCCTGTCTC CTCTCCCTCTGCAGACAGATTTCCAACCACAGCTGGGGATGAGGGAGGAGGGCTGGCCTACCCTAGGCCT CAGAGGTCTCTGTTATCCCTGGTTAAGTAAAAGATCAGCCACCACAGAAGAGTAGAGCAGGG
Notes:	The probe template was PCR amplified from IMAGE:314745 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:314745 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6740 same embryo
	EMAGE:6738 same embryo
	EMAGE:6739 same embryo
	EurExpress:euxassay_007373 same experiment
	MGI:4825177 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source