EMAGE mouse gene expression spatial database

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EMAGE:6810

Bach2 BTB and CNC homology 2 ( MGI:894679)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002436. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002436_01	euxassay_002436_02	euxassay_002436_03	euxassay_002436_04

euxassay_002436_05	euxassay_002436_06	euxassay_002436_07	euxassay_002436_08	euxassay_002436_09

euxassay_002436_10	euxassay_002436_11	euxassay_002436_12	euxassay_002436_13	euxassay_002436_14

euxassay_002436_15	euxassay_002436_16	euxassay_002436_17	euxassay_002436_18	euxassay_002436_19

euxassay_002436_20	euxassay_002436_21	euxassay_002436_22	euxassay_002436_23	euxassay_002436_24

euxassay_002436_25	euxassay_002436_26	euxassay_002436_27

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6810_wholemount_strong.wlz

6810_wholemount_moderate.wlz

6810_wholemount_weak.wlz

6810_wholemount_possible.wlz

6810_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6810_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

strong

regional

strong expression: see section 06 07 09 18 19 weak expression: see section 20 21

cerebral cortex marginal layer

strong

regional

strong expression: see section 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 moderate expression: see section 06 07 08 09 10 11

telencephalon mantle layer

strong

regional

strong expression: see section 21 22 23 24 25 moderate expression: see section 05 06 07

telencephalon marginal layer

moderate

regional

moderate expression: see section 08

olfactory cortex marginal layer

strong

regional

strong expression: see section 15 moderate expression: see section 11 12 weak expression: see section 16 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 06 weak expression: see section 07 08 09 10 19 21 22 23 24

dorsal root ganglion

moderate

regional

moderate expression: see section 11 12 weak expression: see section 13 14 15 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1953
Entity Detected:	Bach2, BTB and CNC homology 2 ( MGI:894679)
Sequence:	sense strand is shown >T1953 GGCCTCNAGGCCAGATTCGGCACGAGGGTTTTTTTTTTCTCAGTGGCCCATGTCACAAACCCTATCTCAG GAATTTCTTCTGAATGTTCAATTTTTTTCATTGAAGACAGCTTCTATACACATCAAAGTTTTATAGCTAT ACTGTGTACATAGTATATATAATATATATGAAATATATATCCATATGCAAAAAGTCCTGCATGCCTCAAT TTTCTCATCCCTGAAACTGGAAGCTTCACTTATCATTTACAAACAGGTTCCAACATTCCTCTTTTTGTGT CTGGTGCCAGAACTGGTTTGGAAGCTGTTAACATGGCTGTTTTGCTTGCTGCACAATTCGGTTTCCATCT GTGCTTATTTACAGACAAAATTCAATGTTGGGAGATGCTTCTCAAGGTTCAATCTCAGACCTTTTACTTT CGTTGGTTTGGTTTTGGTGCCGCCGAACGGTGTCAGGTGAGCACCGTGAGTCCGCTCTTCTCCCCTTGTG TTTTCCCCTCGTCTCTGCGGATACTGTACAGCAATGGTCAACTTTGCCACTTGCACTGAGTTTTGAGTCA AACCTATTT
Notes:	The probe template was PCR amplified from IMAGE:640200 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:640200 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6809 same embryo
	EMAGE:6808 same embryo
	EurExpress:euxassay_002436 same experiment
	MGI:4823382 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source