EMAGE mouse gene expression spatial database

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EMAGE:6968

Prlr prolactin receptor ( MGI:97763)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002432. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002432_01	euxassay_002432_02	euxassay_002432_03	euxassay_002432_04

euxassay_002432_05	euxassay_002432_06	euxassay_002432_07	euxassay_002432_08	euxassay_002432_09

euxassay_002432_10	euxassay_002432_11	euxassay_002432_12	euxassay_002432_13	euxassay_002432_14

euxassay_002432_15	euxassay_002432_16	euxassay_002432_17	euxassay_002432_18	euxassay_002432_19

euxassay_002432_20	euxassay_002432_21	euxassay_002432_22	euxassay_002432_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6968_wholemount_strong.wlz

6968_wholemount_moderate.wlz

6968_wholemount_weak.wlz

6968_wholemount_possible.wlz

6968_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6968_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

strong

regional

strong expression: see section 09 10 moderate expression: see section 11 17 18 19

thyroid gland

strong

regional

strong expression: see section 10 11 12 15 16

testis

moderate

regional

moderate expression: see section 06 07 08 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5119
Entity Detected:	Prlr, prolactin receptor ( MGI:97763)
Sequence:	sense strand is shown >T5119 AATAATCAAAACCAATCCCTTGCATCTCAGTGAGGTTTTCAGGCAATTATAAAAATGCAGAGCCACCCAC ATTCCTGTGCTCATCCTGTTTCACTCTTTAAAGTCTCAGCACCCATTTGTCTCTACATACTGAACATGTA TTTCATATTATATGGACACCATGTAAGTCAGACTCTAGGATCATATGGTGATATGGGTGCTGAGGTAAGA TATAGCGCTAAGAAAGACACAGGAGATGAGACACAAGTCTTTAGCCATGCTCATTCCTACATGGAGCCAA CAGCCTCAAACTTCTGTTCTTGTATTTGGTTGTTGCCTGTTTTCTCATCATCCTGTTTAATGAATAACCA TTGCCCATATAGATTAAAGAATCTTTTGCGCTGAATCATTTCAAAATGTATAGTTTTCTAAGCAACTCTC TGAAAAATTTCAAGTTATGAAGTACATGGAAACTAAATATATTCATTTGGATGGCTGATGGAACTTTAGG ACACAAATGGCAGAATGTTCTCTGATAAGTTTTAACAAGTACATAGAAGGAACTTCATATGAGCACAACT TACCATCAGCAATGCCACTGACTTTTAATAGATGATACATTCTCCTAAGTGCCTGAGACTAATCATGGTG GTCAGTTATAGGTCAATGATTCACTGTAAACCAGTTTGGTGGAAAACAGTCAAGCCCATGTTGCATGTGT AAAATTAAAGCCGGGGGACTCTTTACTTGATCCAAGGTGAATCCTCAGTCAATGAACAACCAAAGGACCT GAACTCCAGGTTAAAGTTTGTCCTGATGGAAAACTAGCTCCTCCATTTTCATTCTGCTTTAGCTGCTCAT CATTTGTAAAATGACCCAGCCTGCCCTTAGCAACTTCAATTTAGCATAGACAAGGGAGAAAGAGATCTGT ATATGTTTTACAAAGTGCCTTTCTTTGCTTCTAAAAGGTTTCATTTTGGAAGAAAAGTGCATTAATTGGA AAGCAAATTTTATCTTAAATGGATTTCTTTTAAATAAATGGAGAGATGACCAAAAAAAGGAGGGGAGATG ACAAAATGTTCTAAAGACTAACGAACAAAATGTGCAGGGATTTTTGTGTATAGTTTAACTGAATCATCAG AGAACTGAAGTGAATAGTATCAATCATTTTGCCTGAGTCTGGGCGTGATTGGAGATGAACCCAGGAGCCT GTAATTCAAGACTTTCCATTCTGAAAACAATGTTGATTCATTCTTGAATGACACTGAATTGTATGCCTGG CCCCGAAGAGGCCAATGTGTGCACTTTGAAGCTCTTCACCCAGATGGAAAAGTAGTAATGACCTTGAATA TCTATCCTGTGACTTGTTATGGATGAAAGCTTTATATATACATCATCAAACAAGGTTTCTTTTGTGACTT TTTTTGGCTCAAGCACAATACTGATTCTGGTAGGAGAGTCTCTGAGCCCTCAGTGTCCAGCTCTGGGCAT GTTACTGCTACTAAATAGGCTGCAAGACCTTGATTTTTTTTTTTAATTCTGACCACTTGTTTCCCCTCTT GTAGGTTAACCCTCCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5337438 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5337438 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6964 same embryo
	EMAGE:6966 same embryo
	EMAGE:6965 same embryo
	EMAGE:6963 same embryo
	EMAGE:6967 same embryo
	EurExpress:euxassay_002432 same experiment
	MGI:4827429 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source