EMAGE mouse gene expression spatial database

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EMAGE:7151

Arpp21 cyclic AMP-regulated phosphoprotein, 21 ( MGI:107562)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002589. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002589_01	euxassay_002589_02	euxassay_002589_03	euxassay_002589_04

euxassay_002589_05	euxassay_002589_06	euxassay_002589_07	euxassay_002589_08	euxassay_002589_09

euxassay_002589_10	euxassay_002589_11	euxassay_002589_12	euxassay_002589_13	euxassay_002589_14

euxassay_002589_15	euxassay_002589_16	euxassay_002589_17	euxassay_002589_18	euxassay_002589_19

euxassay_002589_20	euxassay_002589_21	euxassay_002589_22	euxassay_002589_23	euxassay_002589_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7151_wholemount_strong.wlz

7151_wholemount_moderate.wlz

7151_wholemount_weak.wlz

7151_wholemount_possible.wlz

7151_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7151_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 15 16 21 22 23 24 weak expression: see section 17 18 19 20

hand mesenchyme

moderate

regional

moderate expression: see section 01 02 23 24

foot mesenchyme

moderate

regional

moderate expression: see section 06 07 17 18

lower leg mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 19 20 21 22 23 24

upper leg mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 19 20 21 22 23 24

head mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 23 24

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 23 24

thymus primordium

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

hypothalamus mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 14 15 16 17 18

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 24

telencephalon marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 24

olfactory cortex marginal layer

strong

regional

strong expression: see section 09 10 11 12 15 16 17 18

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 07 08 09 moderate expression: see section 10 11 12 15 16 17 18 19

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 07 08 09 moderate expression: see section 10 11 12 13 14 15 16 17 18 19

rest of cerebellum mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 moderate expression: see section 10 11 12 15 16 17 18 19 20 21 22

pons mantle layer

strong

regional

strong expression: see section 06 07 08 09 moderate expression: see section 10 11 12 13 14 15 16 17 18 19 20

midbrain mantle layer

strong

regional

strong expression: see section 06 07 08 09 moderate expression: see section 10 11 12 13 14 15 16 17 18 19 20 21

dorsal grey horn

strong

regional

strong expression: see section 10 11 12 13 14 15 16

tongue

moderate

regional

moderate expression: see section 10

tongue muscle

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

lower lip

moderate

regional

moderate expression: see section 05 06 07 08 09 10 18 19 20 21 22

upper lip

moderate

regional

moderate expression: see section 05 06 07 08 09 10 18 19 20 21 22

tail

strong

regional

strong expression: see section 10

tail mesenchyme

strong

regional

strong expression: see section 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5251
Entity Detected:	Arpp21, cyclic AMP-regulated phosphoprotein, 21 ( MGI:107562)
Sequence:	sense strand is shown >T5251 GGCAGCTTGGGACAGGTGGAGCTGGAGCAAGCTGGGAGCGAGATTTGCTGGAAGCTGGTTGTCGACCATG TGTCAAGCTGTGTAAGGAAACTGCCTCGAGATGGACACTCTGGACTGCTAATGGGGACATGGGACGGCAG TTACCTCTGATCACTTGCGTGGATTCTCCCGGAACAGAACGACAGAAGCCGCCAGTCATTCGCCCAGATC TACAGCTGGAATTCTGTGCTGAAGGGTCTAAAAACTCTTTTTAATTTGGATCTGGGAGAATGTCTGAGCA AGGAGGACTGACTCCGACCATACTGGAAGAAGGGCAGACGGAGCCAGAGTCTGCCCCAGAAAATGGCATC CTCAAGTCAGAAAGTCTGGATGAGGAGGAGAAGCTGGAACTGCAGCGGCGACTGGCGGCTCAGAACCAAG AGAGGAGAAAATCCAAGTCAGGAGCAGGCAAAGGGAAGCTGACCAGAAGTCTTGCTGTCTGTGAAGAGTC TTCAGCTAGATCTGGAGGGGAAAGTCACCAGGATCAGACTCTCTGAGAACTGCAAATGGAAAGGAACTCA AAAGAATTTAGATTAAAACTTAACTTAAAATAAAATAAAATAAAATAAAAATCCAGTAAGCACGGTAGTG CTGGATATCCACCAAGGCTCTCTCTTGATAAGGCTGAACCAAATAGAACCCAAGCATCCTGTCTTTCTGG AGACAGCTTACCTCTCAGCTCCCAAATGAACTTGCCTCTAAGTAAAACACCACTGTTGGCTCCTAGGGGA TGTAAGAAGTAGTGTCTAAGCACCCTGGACTGTGGTAAAGTGCCGGAATGGACGTCAGGAGCTTTTTATT TTTATTTTATTTTTTTGACTACTGCAAAAACGGTCCGGAAAAGCATCTGACCTGGTGGCAAATTTGATAT TAGCCAGCTGCTACATATCTCCACCCTTATCTTTGATTCCCAAAACTTAAAATTCCAAGAACCTTCACAC CCTCTTGTTACTCCTTTTTTTTCCTTTTTAACTATTATATTTATCTGCGTATGGAAAACATAGTCTTGAT GCTGAAATTTTGCTTATTTTTCTCAGAGGTCAAAGGCAGCCAAGACAAAAGGCAGATGATCTGTCTGAGC CCAGAGGAAGGGAAATTGATTACAAAATCATTCATTTTTGTTTGGCTTTCTTTGGCTTCTCTTTTATCTT CCCACCCCCCTTTCTGCTTTAATTGAAATCCTGTAATTCTCCTTATGGACACAGAGAACATGGAGAGTGC TTTCTTTAAAAAAGAACCAAAAGAAAAAAAAATCCTTATAGATGTTTGTCCTAGGAGTGTTTCCTTTATT AGTCTCATTTTCTTCACATGCCGTTCATGTGGAGAGGCACAGACAAGCTCTCGTTTGGTAGCCATTACTA TGTGTCATAGACAGCCCCCTACCCCCTTTTTTAAAACTTGGGCTCTGAGAAACGTGTGGCTTTCCTTCAG CATTTTATGTGTGCTTCTATTTTTAACGGAGATGGGAAAGGTGGAATGGTGTGAATCCCATGCCTTGTAC TGTCAATGTAGATGGGTGGCTTGTGATGTACAGCATCTGACTTGTGGGAAACCCTGTACAGTGCCAGGCT CCGGGAGAAGTAAGGACCCAGTCGTCCCTCACTCCTAATGCCAAGCAGGGGAGGCCAGCCTCTGTAGATT TCATTTGCTCTTTGTCTCTTTTGACAAGGCACAGGAGTCTTGGTAAGAGCCATCTGTGCCATAGCACCCA TTTCAGAGGCAGGTCTAGTGGTACCTCCACACAGTGGGTGAAAACGGTGCTTTGTTCCTGCTGGTTCTGA ACCCAGAATACAGGATTCATGTTGGAGCAGAGAATTAGGCCCCTCCAGCTTTCTGTGGACTAGCCAAAGA GTGGTGGGAATAGGGGGTTGGCAGAAAACCAACAGACACATATATAATCAACTCACCCGATCTTAAGAAT TTATACGCAATGCCTTCACTAGATAACACTGAACACTTAGAAAATGTGTGGACTGACTCCCAGAGAAGTA TACAGTACATGGAATGTATTGCTTCCCCCGTGTTTGTATATTCACAGTATTTGGGAAACTGCCTTCATTT TCCTGTGTGGGAAAAGCTTTCTTGTCCCCCATGTTACTTGATCTCAGACCCACACCTGATGGTTCAGTCT GTCTTCGAGTAAAGGAAGCGTGTTTTTTTTTCTAATGTTATAATATTTACTGACCAGTGTGTTACTGCAA CTTGAATCATTCTTTTACTGTTGTTGGGTATAAACTTATCCTGTACCAATGTATTTATTAACACTTGTAT TTTAATATTGAGCGTATCAATAAAAATATTTAAAAATAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5358309 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5358309 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7154 same embryo
	EMAGE:7153 same embryo
	EMAGE:7152 same embryo
	EMAGE:7155 same embryo
	EMAGE:7156 same embryo
	EurExpress:euxassay_002589 same experiment
	MGI:4823255 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source