EMAGE mouse gene expression spatial database

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EMAGE:7159

Atp11a ATPase, class VI, type 11A ( MGI:1354735)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004483. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004483_01	euxassay_004483_02	euxassay_004483_03	euxassay_004483_04

euxassay_004483_05	euxassay_004483_06	euxassay_004483_07	euxassay_004483_08	euxassay_004483_09

euxassay_004483_10	euxassay_004483_11	euxassay_004483_12	euxassay_004483_13	euxassay_004483_14

euxassay_004483_15	euxassay_004483_16	euxassay_004483_17	euxassay_004483_18	euxassay_004483_19

euxassay_004483_20	euxassay_004483_21	euxassay_004483_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7159_wholemount_strong.wlz

7159_wholemount_moderate.wlz

7159_wholemount_weak.wlz

7159_wholemount_possible.wlz

7159_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7159_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thyroid gland

strong

regional

strong expression: see section 12 15 16 17 moderate expression: see section 11 13 14

telencephalon meninges

moderate

regional

moderate expression: see section 04 18 22

hindbrain meninges

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22

facial vii ganglion

moderate

spotted

moderate expression: see section 04 05 22

glossopharyngeal ix ganglion

moderate

spotted

moderate expression: see section 07 20

trigeminal v ganglion

moderate

spotted

moderate expression: see section 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

moderate

spotted

moderate expression: see section 08 09 19

vestibulocochlear viii ganglion

moderate

spotted

moderate expression: see section 04 05 22

dorsal root ganglion

weak

regional

weak expression: see section 09 10 11 12 15 16 17 18 19 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 14 15 16 17 18

midgut

strong

regional

strong expression: see section 07 08 09 moderate expression: see section 06 10 11 12 13 14 15 16

urinary system

moderate

spotted

moderate expression: see section 05 06 07 20 21

bladder

strong

regional

strong expression: see section 12 13 14

kidney calyx

moderate

regional

moderate expression: see section 07 08 09 18 19 20 weak expression: see section 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6377
Entity Detected:	Atp11a, ATPase, class VI, type 11A ( MGI:1354735)
Sequence:	sense strand is shown >T6377 GATGTCCAGCTCTGCTGAGGGCTGTCTGTGCCTCCACACACCCTCATGCCCATTCACCCACCTGTGTCGC CATGTGTCAGAGGATTGGAGCCCCAATCCACACTGGCCCTTAATTAGTAGTGTGTGCATTTTTACAGCTC CTTTTTAAGAACCCAGACAGGCACAAATATAATACCTGTGGGGAGGGGCAGATTCTCTCTGTGTTTAGTT AACAGATTATTTGTAATGTATTTTTTTAGGGATCTTAAAATTACCTTTGCACTAAAGTATTTTCATAGCT GTTTCTATATCTTACTCATTTGTTGATGCACCTTCTGATTTTTAAATATGTTTTTAAAGCTTTCACTTTT TAAGTTTAAGAATTTTGGCCACTTAAATGCTGGTGAGCGTTTCGGCTGAATGTCTCAGTGTCTGGCAGCG ACCTCTCTGATTCTCCTCTGTGCCTCTCTCTTTCTTGATGTTTCCAGAGACTGACCAGCTGAGATGTGCT GAGGGCACACCATCTCCTCTGCTTGCTTTGTCACAGTCCTCTGTCACGTCACGGTTTCTATTTTGATTTT GTTTTTTATTTCCATCCTTCAGCCTTAAACACCTAAACAACATCCTTCCTGCAGGGCCTCGATTCTGCA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:ATAAACACCACACCAAAGATG; Reverse Primer - name:unspecified, sequence:TGGAACATACAGGAGATGAAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7157 same embryo
	EMAGE:7161 same embryo
	EMAGE:7158 same embryo
	EMAGE:7162 same embryo
	EMAGE:7160 same embryo
	EurExpress:euxassay_004483 same experiment
	MGI:4823300 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source