EMAGE mouse gene expression spatial database

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EMAGE:7164

0610010F05Rik RIKEN cDNA 0610010F05 gene ( MGI:1918925)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002579. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002579_01	euxassay_002579_02	euxassay_002579_03	euxassay_002579_04

euxassay_002579_05	euxassay_002579_06	euxassay_002579_07	euxassay_002579_08	euxassay_002579_09

euxassay_002579_10	euxassay_002579_11	euxassay_002579_12	euxassay_002579_13	euxassay_002579_14

euxassay_002579_15	euxassay_002579_16	euxassay_002579_17	euxassay_002579_18	euxassay_002579_19

euxassay_002579_20	euxassay_002579_21	euxassay_002579_22	euxassay_002579_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7164_wholemount_strong.wlz

7164_wholemount_moderate.wlz

7164_wholemount_weak.wlz

7164_wholemount_possible.wlz

7164_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7164_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 19 20 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 19 20

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 17 18 19 20 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 18

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 18 19 20

spinal cord

strong

homogeneous

strong expression: see section 11 12 13 14 15 16 17 18 19

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 16 17

cervical ganglion

moderate

regional

moderate expression: see section 09 17 18

thoracic ganglion

moderate

regional

moderate expression: see section 14 15 16

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 12 13 14 17 18 19 20

not examined

regional

not examined expression: see section 01 02 21 22 23

neural retina

strong

regional

strong expression: see section 01 02 21 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 15 weak expression: see section 14 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2068
Entity Detected:	0610010F05Rik, RIKEN cDNA 0610010F05 gene ( MGI:1918925)
Sequence:	sense strand is shown >T2068 TGGCCTCGAGGCCAGATTCGGCACGAGGCTGNGGCAAGGCTTGTGCCTGGATTAACGCCCAAAGAGTGTG TGAAAAGGTTTGATGAGCTAAAGAGCTGTGGAAGTTCACCTGTTGACAACCAGTATAACCCTTTAATGGC TACTGGAGAGGGTCCTGTTGAAACTTTAGCCACTTACATTAAATCTTCACTTCTGGACACACAAGGAGAT TTTCAAGAGACTCCTGTTGATCAAGATACAGTTTCTAAAGCAGGAAGACATAGTATAGCTACCACAAGGA ATTGCTCTTCAGAAAGTGAAAACTGTACTGCTCGTAATGCTGGAGAAGAGACTGGAGAATCTGAAGGGCC AAACATGGTGATCCATGTATGTGATGAAGCAAAAAGCTTGAAAGAAGATTTTATTTGCCCACGAGATCTT TTGATATCAGAAATGAAGTACTTTGCTGAATATTTATCCATGGATGCGCAGCGCTGGGAAGAAGTGGACA TTTCAGTTCATTGCGATGTTCACATTTTCAACTGGCTGATAAAATATGTTAAACGGAA
Notes:	The probe template was PCR amplified from IMAGE:821031 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:821031 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7163 same embryo
	EMAGE:7165 same embryo
	EMAGE:7166 same embryo
	EurExpress:euxassay_002579 same experiment
	MGI:4822580 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source