EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7408

Strn4 striatin, calmodulin binding protein 4 ( MGI:2142346)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004734. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004734_01	euxassay_004734_02	euxassay_004734_03	euxassay_004734_04

euxassay_004734_05	euxassay_004734_06	euxassay_004734_07	euxassay_004734_08	euxassay_004734_09

euxassay_004734_10	euxassay_004734_11	euxassay_004734_12	euxassay_004734_13	euxassay_004734_14

euxassay_004734_15	euxassay_004734_16	euxassay_004734_17	euxassay_004734_18	euxassay_004734_19

euxassay_004734_20	euxassay_004734_21	euxassay_004734_22	euxassay_004734_23	euxassay_004734_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7408_wholemount_strong.wlz

7408_wholemount_moderate.wlz

7408_wholemount_weak.wlz

7408_wholemount_possible.wlz

7408_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7408_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

body cavity or lining

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

limb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 17 18 19 20 21 22 23 24

gland

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

alimentary system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

nervous system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

integumental system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

sensory organ system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

cardiovascular system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

urinary system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

reproductive system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

respiratory system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

skeleton

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tail

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

extraembryonic component

moderate

regional

moderate expression: see section 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8692
Entity Detected:	Strn4, striatin, calmodulin binding protein 4 ( MGI:2142346)
Sequence:	sense strand is shown >T8692 GCGGGCGCGCGGGCCGGCCCCGGGGCCTCCATGATGGAGGAGCGAGCGGCCGCCCCGGTCGCATCGGCCG CCTCCTCCTGCCGCCCTCTGGGCTCGGGCACGGCTCCCAACCCGACGGCGGCGGCCCCGGCCTCCAGCCC TGCTCCTGGGCCCGGCCCGGTAGGAAAAGGAGGTGGCGGCGGAGGCAGCCCGGGCCCTACGGCGGGCCCG GAGCCCCTCAGCCTGCCGGGGATCCTGCACTTTATCCAACACGAGTGGGCACGCTTCGAAGCCGAGAAGG CCCGCTGGGAGGCCGAGCGCGCCGAGCTGCAGGCTCAGGTGGCTTTCCTCCAGGGGGAGAGGAAAGGCCA AGAGAATCTAAAGACGGACCTGGTGCGGCGGATCAAGATGCTGGAGTATGCACTAAAGCAGGAGAGGGCC AAATATCATAAACTGAAGTTTGGTACAGACCTGAATCAGGGGGAGAAGAAGACAGATCTGTCAGAACAAG TCTCCAATGGCCCTGTAGAGTCGGTCACACTGGAGAACAGCCCATTGGTGTGGAAGGAGGGTCGACAGCT TCTGCGACAGTACCTGGAAGAGGTGGGCTACACAGATACCATCTTGGACATGCGGTCCAAGCGTGTGCGT TCCCTTCTGGGCCGTTCACTGGAACTCAATGGGGCCGGTGAGCCCGTTGAGGGGGCCCCCAGGGCTTCAC CAGGCCCTGGGGGACTTAGCGGTGGCGAGTCTCTACTGGTAAAACAGATCGAGGAACAGATCAAGAGGAA TGCAGCTGGCAAAGATGGCAAGGAGCGCCTGGGTGGCTCGGTGCTGGAGCAGATACCCTTCCTGCAGAAC TGCGAGGATGAAGACAGCGATGAGGATGATGAACTGGACAGTGTGCAGCATAAGAAGCAACGTGTGAGGC TCCCATCCAAGGCCCTAGTGCCTGAAATGGAGGACGAGGATGAAGAGGATGACTCAGAAGATGCCATCAA TGAGTTTGATTTCCTGGGCTCAGGAGAGGATGGAGAGGGGTCTCCAGATCCTCGACGTTGTACTTCAGAG GGGAACCCCCATGAGCTGGAAAGCCGTCGGGTCAAACTCCAGGGAATTCTTGCTGACCTCCGGGATGTGG ATGGGCTGCCCCCGAAAGTGACTGTCCCACCTCCTGGCACACCCCAGCCCCGGCCTCATGAAGGTTCCTT TGGCTTCTCCTCAGACGTCTTCATCATGGACACTATCGGGGGCGGGGAGGTGAGCCTGGGGGACTTGGCA GATCTCACCGTCACCAATGACAACGACCTCAGCTGTGATCTGTCTGACAGCAAAGACGCTTTCAAGAAGA CATGGAACCCCAAGTTTACCCTCCGCTCACACTACGATGGCATCCGCTCCCTGGCCTTCCACCACAGCCA GTCAGCACTGCTTACTGCCTCTGAGGATGGCACACTCAAACTGTGGAACCTGCAGAAGGCAGTTACGGCC AAGAAAAATGCTGCACTGGATGTGGAGCCGATTCATGCTTTCCGAGCTCACAGGGGCCCGGTGCTGGCAG TCACCATGGGCAGCAACAGCGAGTACTGTTACAGTGGTGGGGCTGATGCCAGGATCCACAGCTGGAAGAT TCCTGACCTCAACATGGACCCATATGATGGCTATGACCCAAGTGTGCTGAGCCATGTCCTGGAAGGCCAT GGGGATGCTGTGTGGGGTCTGGCCTTCAGTCCCACCTCCCAGCGCCTAGCATCCTGCTCTGCCGATGGCA CCGTCCGCATCTGGGATCCCAGCAGCAGTGGTCCAAGCTGCCTCTGTACCTTCCCCATGGATGGAGAACA TGGAATTCCCACCTCAGTGGCCTTCACCAGCACTGAACCTGCCCATGTCGTGGCCTCCTTTCGTTCTGGT GATACCGTTCTTTATGACCTGGAAGCTGGCAGCGCCCTTCTCACATTGGAGTCTCGAGGGAGCAGTGGCC CAGCACAGATCAACCAGGTGGTGAGTCACCCAAGCCAGCCTCTCACCATCACTGCACACGACGACAGGGG CATCCGGTTCCTGGACAATCGAACAGGTAAATCTGTGCATTCCATGGTGGCCCACCTGGATGCAGTCACC TGCCTAGCTGTGGACCCAAATGGCGTGTTCTTGATGTCAGGAAGCCACGACTGTTCTCTGCGTTTATGGA GCCTAGACAACAAGACATGTGTGCAGGAGATCACGGCCCACCGCAAGAAGCATGAGGAGGCCATCCATGC AGTGGCCTGCCATCCCAGCAAGGCACTTATTGCCAGTGCTGGTGCTGATGCCCTAGCCAAGGTCTTCGTA TGATCCCACCTGGCCTCGCCCTGGCTGCACAGTGTCAGGGAGCGGGGCCAGGCAGTGGGGCTGAGGCAGC CCCCAGCCTCACCTTCAGAGGCTCTGAGTCTTGGCCTCTTTCATCTTCCTGGAGCCTGGTGGTGCTAAAG GCCCTTTCCCCAGGAGTCACCCCTCAAGGATGGCCTCTGGGGTTTCTGCTCATGCCCCAACCCCAACTCC TGACATAGCTGTCGTCCTGAGTTTGACACAGCCCAGGCTGGGTGTCCTTTGGGATCTGATTTTGACCAAG ATGAAAGAAGATGCCAGGCTCCCTCCTAGAGTGCCACCCTCCCTCATTCATCAGATGAAGGGCTGTGCAG AGAGGGGTCTCCTGGGGCCCTCCTATGTGGGCCCCAGGTTGTCCTTTTGGACCTCAGTCCATTCCTCTTG CTTCCCAACACTAACCAAGCCTCTGGGGCCAGGCCTTCCTGGGAAGGGCCTCCACTCCTCTGGGGCATTG TCCTAGCCACAGGCCCTCCTTGAGATGTAGAGAGAGGGTGGAGTGGGCCCTGTGCCCCTAGTTCTCTGTA TATCTGTATAAAGGAATGGGATTTTAATGGCACCCACCCCACGTTATCACTGTGTGATAGTCTGTCAGTC TCCTCCTTGCTCCACACTTTGGCCTCTATTTATTTCACTCTTTATTTGGGTCCCACCTTATGCCCTCGGT TCCCCTTTCAGGTTCCTGTTCATAAGCCCCTTCCTTTTTGCCCCAACTTGTATGTGAAAAAAATTGTCTC AATAAACCCTTTGGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6401782 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6401782 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7411 same embryo
	EMAGE:7407 same embryo
	EMAGE:7406 same embryo
	EMAGE:7410 same embryo
	EMAGE:7409 same embryo
	EurExpress:euxassay_004734 same experiment
	MGI:4828519 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source