EMAGE mouse gene expression spatial database

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EMAGE:7446

Mt2 metallothionein 2 ( MGI:97172)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004529. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004529_01	euxassay_004529_02	euxassay_004529_03	euxassay_004529_04

euxassay_004529_05	euxassay_004529_06	euxassay_004529_07	euxassay_004529_08	euxassay_004529_09

euxassay_004529_10	euxassay_004529_11	euxassay_004529_12	euxassay_004529_13	euxassay_004529_14

euxassay_004529_15	euxassay_004529_16	euxassay_004529_17	euxassay_004529_18	euxassay_004529_19

euxassay_004529_20	euxassay_004529_21	euxassay_004529_22	euxassay_004529_23	euxassay_004529_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7446_wholemount_strong.wlz

7446_wholemount_moderate.wlz

7446_wholemount_weak.wlz

7446_wholemount_possible.wlz

7446_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7446_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 weak expression: see section 16 17

pancreas

moderate

regional

moderate expression: see section 08 09 10 11 12

thyroid gland

strong

regional

strong expression: see section 09 10 14 15

vibrissa

strong

regional

strong expression: see section 04 05 11 12 13 18 19 20

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 23 24 weak expression: see section 01 02 03 22

esophagus

moderate

regional

moderate expression: see section 12

stomach

moderate

regional

moderate expression: see section 02 03 04 05 06 weak expression: see section 07 08 09 10

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11

liver right lobe

strong

regional

strong expression: see section 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5767
Entity Detected:	Mt2, metallothionein 2 ( MGI:97172)
Sequence:	sense strand is shown >T5767 CTTCAAACCGATCTCTCGTCGATCTTCAACCGCCGCCTCCACTCGCCATGGACCCCAACTGCTCCTGTGC CTCCGATGGATCCTGCTCCTGTGCTGGCGCCTGCAAATGCAAACAATGCAAATGTACTTCCTGCAAGAAA AGCTGCTGCTCCTGCTGCCCCGTGGGCTGTGCGAAGTGCTCCCAGGGCTGCATCTGCAAAGAGGCTTCCG ACAAGTGCAGCTGCTGTGCCTGAAGGGGGGCGGAGGGGTCCCCACATCTGTGTAAATAGACCATGTAGAA GCCTAGCCTTTTTTGTACAACCCTGACCCGTTCTCCACAACTTTTTCTATAAAGCATGTAACTGACAATA AAAGCCGTTGACTTAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2651471 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2651471 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7447 same embryo
	EMAGE:7443 same embryo
	EMAGE:7445 same embryo
	EMAGE:7444 same embryo
	EurExpress:euxassay_004529 same experiment
	MGI:4826478 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source