EMAGE mouse gene expression spatial database

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EMAGE:7516

Adck4 aarF domain containing kinase 4 ( MGI:1924139)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004577. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004577_01	euxassay_004577_02	euxassay_004577_03	euxassay_004577_04

euxassay_004577_05	euxassay_004577_06	euxassay_004577_07	euxassay_004577_08	euxassay_004577_09

euxassay_004577_10	euxassay_004577_11	euxassay_004577_12	euxassay_004577_13	euxassay_004577_14

euxassay_004577_15	euxassay_004577_16	euxassay_004577_17	euxassay_004577_18	euxassay_004577_19

euxassay_004577_20	euxassay_004577_21	euxassay_004577_22	euxassay_004577_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7516_wholemount_strong.wlz

7516_wholemount_moderate.wlz

7516_wholemount_weak.wlz

7516_wholemount_possible.wlz

7516_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7516_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adenohypophysis

moderate

regional

moderate expression: see section 10 11 12 13 14 15

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 12 13 14

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 13 14 15

lateral ventricle choroid plexus

strong

regional

strong expression: see section 08 09 10 moderate expression: see section 11 13 14 15 16 17 18 19 20

4th ventricle lateral recess

strong

regional

strong expression: see section 04 05 06 07 08 moderate expression: see section 10 11 12 13 14 15 16 17 18 19 20 21

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 11 12 13 14 15

pons ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18

spinal cord ventricular layer

moderate

regional

moderate expression: see section 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7593
Entity Detected:	Adck4, aarF domain containing kinase 4 ( MGI:1924139)
Sequence:	sense strand is shown >T7593 CGCTTGGCCAGCTTTGGGGGGCTGGCTGTGGGCTTGGGCCTGGGAGCGCTGGCTGAGGTGACCAAGAAGT CCTTGCCAGGTGGAAGTCTGCAGCATGAGGGCGTCTCTGGATTGGGCTCCAGTCCTTTCCTCTCCGAGGC CAATGCTGAGAGGATCGTGCAGACCTTATGTACAGTCCGGGGGGCTGCCCTCAAGATCGGCCAAATGCTC AGTATCCAGGACAACAGCTTTATCAGCCCCCAGCTTCAGCGAATCTTTGAGAGGGTCCGACAGAGTGCCG ACTTTATGCCCCGCTGGCAAATGATGAGAGTTCTAGAAGAGGAGCTAGGCAAGGACTGGCAAGACAAGGT GGCCTCCCTCGAGGAAGTGCCCTTTGCTGCTGCCTCCATTGGGCAGGTACACCAAGGCTTGCTAAAGGAT GGGACTGAGGTGGCTGTGAAGATCCAGTACCCAGGTGTTGCCCAGAGCATCCAGAGTGACGTGGAGAACC TGCTGGCCCTGCTCAAGATGAGTGTGGGCCTGCCAGAGGGCCTGTTTGCTGAGCAGAGCCTGCAGACCTT GCAGCAGGAGCTGGCTTGGGAATGTGACTACCGCAGAGAAGCGGCCTGTGCCCAGACCTTCAGGAAGCTC TTAGCTGACGACCCCTTCTTCCGAGTGCCAGCTGTGGTGCAGGAGCTATGCACTACACGGGTGCTGGGCA TGGAGCTGGCTGGGGGAATCCCTCTAGACCAGTGCCAGGGCCTGAGCCAAGACATCCGGAATCAGATCTG CTTTCAGCTCCTGAGGTTGTGTCTGCGGGAACTGTTTGAGTTCAGATTCATGCAGACTGATCCCAACTGG GCCAACTTCCTGTATGATGCCTCCAGCCACCAGGTGACTCTACTGGATTTTGGTGCAAGCCGGGCGTTTG GGACAGAGTTCACGGACCATTACATTGAGGTGGTGAAGGCTGCAGCAGATGGAGACAGAGATCGAGTTCT ACAGAAATCCCAGGACCTCAAGTTCCTTACAGGCTTTGAAACCAAGGCATTCTCTGACGCCCACGTCGAG GCAGTGATGATCCTGGGGGAGCCCTTTGCTGCCTCAGGCCCCTACGACTTTGGGGCTGGAGAGACTGCTC GCCGAATACAGGGCCTCATCCCGGTATTGCTTCGGCACCGGCTGCGCCCACCGCCTGAGGAGACCTATGC TCTACACCGCAAGCTAGCTGGGGCTTTCTTAGCCTGTGCCCGTCTCCACGCCCACATCGCCTGCCGGGAC CTCTTCCAAGATACCTACCACCGCTACTGGGCCAGTCGCCAGACACTGCCGCTGCCAGCAGCCTCCTGAC TAGGGACCTCCCTGCCGACCCCACCATGACAGATTCCATCCAGGGATACCAGCTCCACAGCAGGCAGTCC CCTCCTCCTCTTTCTTGGTCTCCAGAACCTAGTTTCTTGCCTTGGATCTTTGGGCCCCTGGAGTTAGCTC CCTGATCCTAAGTGAGCAACCTTGAATTCTGAGGCTAGGAGCCAGCAGAATCCTGCCACCATCTAAGTGA GCCTTGGGTCCACTCTCATGTGAAGGAGACGCCCCTCGGCCCCAGGTCTTCCAGGATCTGCAGGCGAAAG GGGAGATGGTGGCTGTTGGGGGCGGTCCTCACCTTTGCCTCCCTTGCTCCTCTACCAGCTGCCTTCTTCC CGGGTTCCAGCCTGTGTATTGGAGGGTGGGGGTGGGTGCTGATCCTTTCCTCCGAATAAAGGCACCCTTT CCCTGCAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4242474 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4242474 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7514 same embryo
	EMAGE:7515 same embryo
	EMAGE:7512 same embryo
	EMAGE:7513 same embryo
	EMAGE:7511 same embryo
	EurExpress:euxassay_004577 same experiment
	MGI:4822997 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source