Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7550

Usp18 ubiquitin specific peptidase 18 ( MGI:1344364)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7550 EMAGE:7550 EMAGE:7550 EMAGE:7550 EMAGE:7550
"Pseudo-wholemount" of euxassay_005234. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005234_01 euxassay_005234_02 euxassay_005234_03 euxassay_005234_04
EMAGE:7550 EMAGE:7550 EMAGE:7550 EMAGE:7550 EMAGE:7550
euxassay_005234_05 euxassay_005234_06 euxassay_005234_07 euxassay_005234_08 euxassay_005234_09
EMAGE:7550 EMAGE:7550 EMAGE:7550 EMAGE:7550 EMAGE:7550
euxassay_005234_10 euxassay_005234_11 euxassay_005234_12 euxassay_005234_13 euxassay_005234_14
EMAGE:7550 EMAGE:7550 EMAGE:7550 EMAGE:7550 EMAGE:7550
euxassay_005234_15 euxassay_005234_16 euxassay_005234_17 euxassay_005234_18 euxassay_005234_19
EMAGE:7550 EMAGE:7550 EMAGE:7550
euxassay_005234_20 euxassay_005234_21 euxassay_005234_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7550Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7550_wholemount_strong.wlz
7550_wholemount_moderate.wlz
7550_wholemount_weak.wlz
7550_wholemount_possible.wlz
7550_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7550_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
mandible
weak weak
regionalweak expression: see section 01 06 07 08 15 16 17 18
lung
weak weak
regionalweak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
axial skeleton
weak weak
regionalweak expression: see section 07 08 09 10 11 12 13 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2035
Entity Detected:Usp18, ubiquitin specific peptidase 18 ( MGI:1344364)
Sequence:sense strand is shown

>T2035
GGCCTCGAGCCAGATTCGGCACGAGGGTCCAGCACGATGCTGCTCAACTCTACCTTACAATCTGGAACCT
GACTAAGGACCAGATCACGGACACAGACTTGACAGAGCGGCTGCAGGGTCTGTTCACCATCTGGACGCAG
GAGTCCCTGATTTGCGTGGGCTGTACAGCAGAGAGCAGCAGGAGGAGCAAACTGCTCACACTCTCTCTTC
CTCTTTTTGATAAGGACGCAAAGCCTCTGAAAACCCTGGAGGATGCCTTGAGATGTTTCGTCCAGCCCAA
AGAGTTAGCAAGCTCCGACATGTGCTGCGAGAGCTGTGGGGAGAAGACGCCTTGGAAACAGGTTCTGAAG
CTGACTCATCTGCCCCAAACCTTGACCATTCACCTCATGAGATTCTCTGCCAGGAACTCGAGGACGGAAA
AGATCTGTCACTCAGTTAATTTCCCTCAGAGCTTGGATTTCAGTCAGGTTCTGCCAACCGAGGAAGACCT
CGGTGATACCAAGGAACAGTCTGAAATACACTATGAACTCTTTGCCGTGATTGCCCACGTTGGGATGG
Notes:The probe template was PCR amplified from IMAGE:751642 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:751642 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7547 same embryo
 EMAGE:7548 same embryo
 EMAGE:7551 same embryo
 EMAGE:7549 same embryo
 EurExpress:euxassay_005234 same experiment
 MGI:4829129 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS