EMAGE mouse gene expression spatial database

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EMAGE:7810

Lyzl4 lysozyme-like 4 ( MGI:1916282)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005328. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005328_01	euxassay_005328_02	euxassay_005328_03	euxassay_005328_04

euxassay_005328_05	euxassay_005328_06	euxassay_005328_07	euxassay_005328_08	euxassay_005328_09

euxassay_005328_10	euxassay_005328_11	euxassay_005328_12	euxassay_005328_13	euxassay_005328_14

euxassay_005328_15	euxassay_005328_16	euxassay_005328_17	euxassay_005328_18	euxassay_005328_19

euxassay_005328_20	euxassay_005328_21	euxassay_005328_22	euxassay_005328_23	euxassay_005328_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7810_wholemount_strong.wlz

7810_wholemount_moderate.wlz

7810_wholemount_weak.wlz

7810_wholemount_possible.wlz

7810_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7810_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10 17

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 17 18 19 20 21 22 23

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 16 17 18 19

stomach

strong

regional

strong expression: see section 04 moderate expression: see section 05 06 07 08

mandible

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 15 16 17 18 19 20 21

maxilla

moderate

regional

moderate expression: see section 05 06 07 08 09 16 17 19

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6953
Entity Detected:	Lyzl4, lysozyme-like 4 ( MGI:1916282)
Sequence:	sense strand is shown >T6953 ACTTCCCTTGTTCTCTCTCCCCCTGAGCCTGAGCCTGAGCCTGAGCCAGGCCTCTACTACGCACCAGGGC ATAGGAGAACATTCTGGCTCACAGATGTCATAGAGAAGGTTCTAGAAGAGTTTCTCATGGAGCAGGATCG ATGGTGTAGAAACTCTTGGGTGAAGACAAATGGGTTATTAAGAAAGTGGCATCTGAGAAAGTGCAGGGGA CCCAGTGCCGGCAGAGTACCCAGCCTGCAAGCAACGTGGACTCCCTGCCGGGCTGTGCCTCTCCTTTCCC TGGAAAGTCCAGTGTCCCTATGACCTTGGAAGGAGCTGCCTGAGACTCTCTGTTCAGCCGGCATCCACAG CTTCGAGCCACCAGAGCTTGGCGACGATGCAGCTGTACCTGGTGCTTCTCCTCATCAGCTACTTGCTGAC TCCGATCGGTGCTTCCATCTTGGGGCGCTGCACAGTGGCTAAGATGCTCTATGATGGGGGCTTGAATTAT TTTGAGGGCTACAGCCTTGAAAACTGGGTGTGCCTGGCTTATTTTGAGAGCAAGTTCAACCCCTCAGCTG TCTATGAGGACCCACAAGATGGCTCCACCGGTTTCGGCCTCTTTCAGATTCGTGACAATGAATGGTGCGG CCACGGCAAGAACCTCTGCAGTGTGTCCTGCACTGCTTTACTGAATCCAAACTTAAAGGATACGATCCAG TGTGCCAAGAAAATCGTGAAAGGAAAACATGGGATGGGAGCGTGGCCCATCTGGTCCAAGAACTGCCAGC TGTCTGACGTTCTGGACCGGTGGCTGGACGGCTGTGATCTGTAGGCACCCGAGTGGCCCTGCCTCACTTG CCAGCCTCGTCTTGGGACTGAGGATGCTCTCCTGTTTGCTGCTTCAATCCACCCAGTTACCTCACCACTG GCCAGCTCCAGGTGGAAAAGGACGGCAGTCCTCACTTCATCCAGGGGTGCAAGGTGCAGAATAAACCAGC TAATTACTCAGCCTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3985345 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3985345 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7812 same embryo
	EMAGE:7807 same embryo
	EMAGE:7808 same embryo
	EMAGE:7809 same embryo
	EMAGE:7811 same embryo
	EurExpress:euxassay_005328 same experiment
	MGI:4826047 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source