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EMAGE:7815

Klhl35 kelch-like 35 (Drosophila) ( MGI:1919434)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7815 EMAGE:7815 EMAGE:7815 EMAGE:7815 EMAGE:7815
"Pseudo-wholemount" of euxassay_007584. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007584_01 euxassay_007584_02 euxassay_007584_03 euxassay_007584_04
EMAGE:7815 EMAGE:7815 EMAGE:7815 EMAGE:7815 EMAGE:7815
euxassay_007584_05 euxassay_007584_06 euxassay_007584_07 euxassay_007584_08 euxassay_007584_09
EMAGE:7815 EMAGE:7815 EMAGE:7815 EMAGE:7815 EMAGE:7815
euxassay_007584_10 euxassay_007584_11 euxassay_007584_12 euxassay_007584_13 euxassay_007584_14
EMAGE:7815 EMAGE:7815 EMAGE:7815 EMAGE:7815 EMAGE:7815
euxassay_007584_15 euxassay_007584_16 euxassay_007584_17 euxassay_007584_18 euxassay_007584_19
EMAGE:7815 EMAGE:7815 EMAGE:7815
euxassay_007584_20 euxassay_007584_21 euxassay_007584_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7815Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7815_wholemount_strong.wlz
7815_wholemount_moderate.wlz
7815_wholemount_weak.wlz
7815_wholemount_possible.wlz
7815_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7815_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
diencephalon lateral wall ventricular layer
moderate moderate
regionalmoderate expression: see section 11 12 13
rest of cerebellum ventricular layer
moderate moderate
regionalmoderate expression: see section 05 06 10 11 12 13 14 15 16 17 18 19 20 21 weak expression: see section 07 08 09
pons ventricular layer
moderate moderate
regionalmoderate expression: see section 05 06 10 17 20 weak expression: see section 07 08 09
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19
spinal cord ventricular layer
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35390
Entity Detected:Klhl35, kelch-like 35 (Drosophila) ( MGI:1919434)
Sequence:sense strand is shown

>T35390
CTACTTCCTGGAGAAGGTGGAGGCGGACGAGCTGCTGCAGGCGTGCGGCGACTGCCGCCCTCTGCTGCTC
GAAGCCCGCGCCTGCTTCATCCTGGGCCGGGAAGCGGGCGCGCTGCGGGCCAGGCCGCGGAGGTTCATGG
ACTTAGCTGAAGTGATCGTGGTCATTGGCGGGTGTGACCGAAAGGGGCTCCTGAAGCTGCCTTTTGCGGA
TGCTTACCACCCAGAAAGTCAGCGCTGGACCCCACTGCCTAGCCTGCCTGGTTATACACGCTCTGAATTT
GCCTCCTGTGCACTTCGAAATGACATTTACGTTTCTGGAGGTCACATCAACAGCCGTGACGTCTGGATGT
TTAGCTCCCATCTGAACACCTGGATCAAGGTTGCCTCAATGCACAAGGGCAGGTGGAGGCACAAGATGGT
GGCCCTGCAGGGACAGCTCTTTGCAGTAGGTGGCTTCGATGGCCTGAGACGCCTGCGCAGCGTGGAGCGC
TATGACCCGTTCTCCAACACCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 80598. Forward Primer - name:080598_F_cDNA_2810406K13Rik, sequence:CTACTTCCTGGAGAAGGTGGAG; Reverse Primer - name:080598_N_SP6_cDNA_2810406K13Rik, sequence:AGGTGTTGGAGAACGGGTCAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7816 same embryo
 EMAGE:7817 same embryo
 EMAGE:7819 same embryo
 EMAGE:7818 same embryo
 EMAGE:7820 same embryo
 EurExpress:euxassay_007584 same experiment
 MGI:4825803 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS