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EMAGE:7823

Scn10a sodium channel, voltage-gated, type X, alpha ( MGI:108029)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7823 EMAGE:7823 EMAGE:7823 EMAGE:7823 EMAGE:7823
"Pseudo-wholemount" of euxassay_007406. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007406_01 euxassay_007406_02 euxassay_007406_03 euxassay_007406_04
EMAGE:7823 EMAGE:7823 EMAGE:7823 EMAGE:7823 EMAGE:7823
euxassay_007406_05 euxassay_007406_06 euxassay_007406_07 euxassay_007406_08 euxassay_007406_09
EMAGE:7823 EMAGE:7823 EMAGE:7823 EMAGE:7823 EMAGE:7823
euxassay_007406_10 euxassay_007406_11 euxassay_007406_12 euxassay_007406_13 euxassay_007406_14
EMAGE:7823 EMAGE:7823 EMAGE:7823 EMAGE:7823 EMAGE:7823
euxassay_007406_15 euxassay_007406_16 euxassay_007406_17 euxassay_007406_18 euxassay_007406_19
EMAGE:7823 EMAGE:7823 EMAGE:7823
euxassay_007406_20 euxassay_007406_21 euxassay_007406_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7823Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7823_wholemount_strong.wlz
7823_wholemount_moderate.wlz
7823_wholemount_weak.wlz
7823_wholemount_possible.wlz
7823_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7823_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
corpus striatum
weak weak
regionalweak expression: see section 03 05 17 18 19
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 05 06 14 15 16
trigeminal v ganglion
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 14 15 16 17 18 19 20
dorsal root ganglion
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 12 13 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35292
Entity Detected:Scn10a, sodium channel, voltage-gated, type X, alpha ( MGI:108029)
Sequence:sense strand is shown

>T35292
AATCTGGGGAGTTGGATTCTCTGAAGACTAATATGGAAGAGAAGTTTATGGCAACTAATCTTTCCAAAGC
ATCCTATGAACCAATAGCAACCACCCTCCGGTGCAAGCAGGAAGACATCTCAGCCACCATTATTCAAAAG
GCCTATCGGAACTACATGTTGCAACGCTCCTTGATGCTCTCCAACACCCTGCATGTGCCCAGGGCTGAGG
AAGATGGCGTGTCACTCCCCAAGGGAGGCTATGTTACATTCATGGCAAATGACAACGGTGGGCTCCCAGA
CAAATCAGAAACTGCTTCTGCTACGTCTTTCCCACCATCCTATGAAAGCGTCACCAGGGGCCTGAGTGAC
AGGGCCAACATTAACACATCTAGCTCAATGCAAAATGAAGATGAAGTCACTGCTAAGGAAGGGAATAGCC
CTGGACCTCAGTGAAGACACTCCAGCATGCACGGGGCGGGTTCTAATGTCTTTTTCTGGTGTGCTGACCT
TTTCCTTCTAGAGATGGCACCAACCTACAGCCTCCACCGATGCATGTCACTGGTCATGGTGTCAGAGCTG
AATAGGGACATCCTTGAGAAAGCCCCCACCCCAATAGGAATCAAAAGCCAAGAATACTCCCCCACTTTGA
CATCTCTTCTGAACTTTCAGAAGATGACATCGCTTTGTGTTGTTTGTGACCAGAGATGTGATTTACCAAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 98539. Forward Primer - name:098539_F_cDNA_Scn10a, sequence:AATCTGGGGAGTTGGATTCTCT; Reverse Primer - name:098539_N_SP6_cDNA_Scn10a, sequence:GTTGGTAAATCACATCTCTGGTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7822 same embryo
 EMAGE:7824 same embryo
 EMAGE:7826 same embryo
 EMAGE:7821 same embryo
 EMAGE:7825 same embryo
 EurExpress:euxassay_007406 same experiment
 MGI:4827900 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS