EMAGE mouse gene expression spatial database

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EMAGE:7904

Ms4a3 membrane-spanning 4-domains, subfamily A, member 3 ( MGI:2158468)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012966. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012966_01	euxassay_012966_02	euxassay_012966_03	euxassay_012966_04

euxassay_012966_05	euxassay_012966_06	euxassay_012966_07	euxassay_012966_08	euxassay_012966_09

euxassay_012966_10	euxassay_012966_11	euxassay_012966_12	euxassay_012966_13	euxassay_012966_14

euxassay_012966_15	euxassay_012966_16	euxassay_012966_17	euxassay_012966_18	euxassay_012966_19

euxassay_012966_20	euxassay_012966_21	euxassay_012966_22	euxassay_012966_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7904_wholemount_strong.wlz

7904_wholemount_moderate.wlz

7904_wholemount_weak.wlz

7904_wholemount_possible.wlz

7904_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7904_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39081
Entity Detected:	Ms4a3, membrane-spanning 4-domains, subfamily A, member 3 ( MGI:2158468)
Sequence:	sense strand is shown >T39081 GAGGAAGAGACTGCTGGTTTTGGGGGACAGACTCTGGTGGTCATTACTGTCTCCTCTTCTGTAATGAGTT GGACTTGCAGGGGAAGGACTTGTCAAATCCTGAATTCTTCAAGGACTGGGGATCTCGGCAGGTGGTACCT CATCAGGCAGACACATGAAGCCAGAGGAGACTGGTGGTTCTGTTTATCAGCCCTTGGATGAGTCACGCCA TGTTCAACGAGGTGTACTGCAAGCCCTCGGGGCCATCCAGATCCTGAATGGAATACTGATTCTAGCTCTC GGAATTTTTCTGGTTTGTTTACAACACGTGTCCCACCACTTCAGGCATTTCTTCTTCTTCACCTTCTACA CAGGCTACCCACTGTGGGGTGCTGTGTTTTTTATCAGCTCAGGATCCTTGACTGTTGCCGCAGGGAGAAA CCCCACACGAATGCTGATGCAAAACAGTTTTGGGATAAACATTGCCAGTACTACAATTGCATTTGTTGGG ACTGTTTTCCTTTCTGTGCATTTGGCATTCAATACCCAGGCTTTCAAGGGTTGCCAATCTTCACCGTCAC CTGATGTCTGCATTTCCCTGGGTTCCTCATCAGATGGCCTGGTGTCTTTAATGCTGATTCTCACCCTGCT GGAGCTGTCCGTGACCATTTCTATCTCTGCCATGTGGTGCTTGGGAAATGTTTGTGGTTTAAGAGAGGCA ATTACTTCACCTCCTAATTCTGTGGAGTCAGGAATACTTCCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 95575. Forward Primer - name:095575_F_cDNA_Ms4a3, sequence:GAGGAAGAGACTGCTGGTTTTG; Reverse Primer - name:095575_N_SP6_cDNA_Ms4a3, sequence:AGGAAGTATTCCTGACTCCACA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7905 same embryo
	EMAGE:7903 same embryo
	EMAGE:7906 same embryo
	EurExpress:euxassay_012966 same experiment
	MGI:4826462 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source